Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape

The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid rece...

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Autores principales: Stortz, M., Presman, D.M., Bruno, L., Annibale, P., Dansey, M.V., Burton, G., Gratton, E., Pecci, A., Levi, V.
Formato: JOUR
Materias:
glucocorticoid receptor
NCOA2 protein, human
nuclear receptor coactivator 2
protein binding
binding site
cell culture
cell nucleus
chromatin
gene expression regulation
genetic transcription
genetics
human
metabolism
promoter region
transcription initiation
Binding Sites
Cell Nucleus
Cells, Cultured
Chromatin
Gene Expression Regulation
Humans
Nuclear Receptor Coactivator 2
Promoter Regions, Genetic
Protein Binding
Receptors, Glucocorticoid
Transcription, Genetic
Transcriptional Activation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Stortz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_20452322_v7_n1_p_Stortz
record_format dspace
spelling todo:paper_20452322_v7_n1_p_Stortz2023-10-03T16:38:21Z Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape Stortz, M. Presman, D.M. Bruno, L. Annibale, P. Dansey, M.V. Burton, G. Gratton, E. Pecci, A. Levi, V. glucocorticoid receptor NCOA2 protein, human nuclear receptor coactivator 2 protein binding binding site cell culture cell nucleus chromatin gene expression regulation genetic transcription genetics human metabolism promoter region transcription initiation Binding Sites Cell Nucleus Cells, Cultured Chromatin Gene Expression Regulation Humans Nuclear Receptor Coactivator 2 Promoter Regions, Genetic Protein Binding Receptors, Glucocorticoid Transcription, Genetic Transcriptional Activation The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid receptor (GR) in the interphase nucleus of living cells. We found that this ligand-activated transcription factor diffuses within the nucleus and dynamically interacts with bodies enriched in the coregulator NCoA-2, DNA-dependent foci and chromatin targets. The distribution of the receptor among the nuclear compartments depends on NCoA-2 and the conformation of the receptor as assessed with synthetic ligands and GR mutants with impaired transcriptional abilities. Our results suggest that the partition of the receptor in different nuclear reservoirs ultimately regulates the concentration of receptor available for the interaction with specific targets, and thus has an impact on transcription regulation. © 2017 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Stortz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic glucocorticoid receptor
NCOA2 protein, human
nuclear receptor coactivator 2
protein binding
binding site
cell culture
cell nucleus
chromatin
gene expression regulation
genetic transcription
genetics
human
metabolism
promoter region
transcription initiation
Binding Sites
Cell Nucleus
Cells, Cultured
Chromatin
Gene Expression Regulation
Humans
Nuclear Receptor Coactivator 2
Promoter Regions, Genetic
Protein Binding
Receptors, Glucocorticoid
Transcription, Genetic
Transcriptional Activation
spellingShingle glucocorticoid receptor
NCOA2 protein, human
nuclear receptor coactivator 2
protein binding
binding site
cell culture
cell nucleus
chromatin
gene expression regulation
genetic transcription
genetics
human
metabolism
promoter region
transcription initiation
Binding Sites
Cell Nucleus
Cells, Cultured
Chromatin
Gene Expression Regulation
Humans
Nuclear Receptor Coactivator 2
Promoter Regions, Genetic
Protein Binding
Receptors, Glucocorticoid
Transcription, Genetic
Transcriptional Activation
Stortz, M.
Presman, D.M.
Bruno, L.
Annibale, P.
Dansey, M.V.
Burton, G.
Gratton, E.
Pecci, A.
Levi, V.
Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
topic_facet glucocorticoid receptor
NCOA2 protein, human
nuclear receptor coactivator 2
protein binding
binding site
cell culture
cell nucleus
chromatin
gene expression regulation
genetic transcription
genetics
human
metabolism
promoter region
transcription initiation
Binding Sites
Cell Nucleus
Cells, Cultured
Chromatin
Gene Expression Regulation
Humans
Nuclear Receptor Coactivator 2
Promoter Regions, Genetic
Protein Binding
Receptors, Glucocorticoid
Transcription, Genetic
Transcriptional Activation
description The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid receptor (GR) in the interphase nucleus of living cells. We found that this ligand-activated transcription factor diffuses within the nucleus and dynamically interacts with bodies enriched in the coregulator NCoA-2, DNA-dependent foci and chromatin targets. The distribution of the receptor among the nuclear compartments depends on NCoA-2 and the conformation of the receptor as assessed with synthetic ligands and GR mutants with impaired transcriptional abilities. Our results suggest that the partition of the receptor in different nuclear reservoirs ultimately regulates the concentration of receptor available for the interaction with specific targets, and thus has an impact on transcription regulation. © 2017 The Author(s).
format JOUR
author Stortz, M.
Presman, D.M.
Bruno, L.
Annibale, P.
Dansey, M.V.
Burton, G.
Gratton, E.
Pecci, A.
Levi, V.
author_facet Stortz, M.
Presman, D.M.
Bruno, L.
Annibale, P.
Dansey, M.V.
Burton, G.
Gratton, E.
Pecci, A.
Levi, V.
author_sort Stortz, M.
title Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_short Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_full Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_fullStr Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_full_unstemmed Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_sort mapping the dynamics of the glucocorticoid receptor within the nuclear landscape
url http://hdl.handle.net/20.500.12110/paper_20452322_v7_n1_p_Stortz
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