ESIPT and FRET probes for monitoring nanoparticle polymer coating stability

Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensivel...

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Autores principales: Azcárate, J.C., Díaz, S.A., Fauerbach, J.A., Gillanders, F., Rubert, A.A., Jares-Erijman, E.A., Jovin, T.M., Fonticelli, M.H.
Formato: JOUR
Materias:
Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_20403364_v9_n25_p8647_Azcarate
record_format dspace
spelling todo:paper_20403364_v9_n25_p8647_Azcarate2023-10-03T16:37:47Z ESIPT and FRET probes for monitoring nanoparticle polymer coating stability Azcárate, J.C. Díaz, S.A. Fauerbach, J.A. Gillanders, F. Rubert, A.A. Jares-Erijman, E.A. Jovin, T.M. Fonticelli, M.H. Biocompatibility Coatings Energy transfer Excited states Hydrophobicity Ionic strength Nanoparticles Polymers Stability System stability Adjustable parameters Amphiphilic polymers Coating instability Complex environments Excited-state intramolecular proton transfer Hydrophobic interactions Inorganic nanoparticle Resonance energy transfer Plastic coatings Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensively used to transfer NPs to aqueous media. For applications of coated NPs as actuators (sensors, markers, or for drug delivery) in a complex environment, such as biological systems, it is important to achieve a deep understanding of the factors affecting coating stability and behavior. We have designed a system that tests the coating stability of amphiphilic polymers through a simple fluorescent readout using either polarity sensing ESIPT (excited state intramolecular proton transfer) dyes or NP FRET (Förster resonance energy transfer). The stability of the coating was determined in response to changes in polarity, pH and ionic strength in the medium. Using the ESIPT system we observed linear changes in signal up to ∼20-25% v/v of co-solvent addition, constituting a break point. Based on such data, we propose a model for coating instability and the important adjustable parameters, such as the electrical charge distribution. FRET data provided confirmatory evidence for the model. The ESIPT dyes and FRET based methods represent new, simple tools for testing NP coating stability in complex environments. © 2017 The Royal Society of Chemistry. Fil:Fauerbach, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
spellingShingle Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
Azcárate, J.C.
Díaz, S.A.
Fauerbach, J.A.
Gillanders, F.
Rubert, A.A.
Jares-Erijman, E.A.
Jovin, T.M.
Fonticelli, M.H.
ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
topic_facet Biocompatibility
Coatings
Energy transfer
Excited states
Hydrophobicity
Ionic strength
Nanoparticles
Polymers
Stability
System stability
Adjustable parameters
Amphiphilic polymers
Coating instability
Complex environments
Excited-state intramolecular proton transfer
Hydrophobic interactions
Inorganic nanoparticle
Resonance energy transfer
Plastic coatings
description Coating strategies of inorganic nanoparticles (NPs) can provide properties unavailable to the NP core alone, such as targeting, specific sensing, and increased biocompatibility. Non-covalent amphiphilic NP capping polymers function via hydrophobic interactions with surface ligands and are extensively used to transfer NPs to aqueous media. For applications of coated NPs as actuators (sensors, markers, or for drug delivery) in a complex environment, such as biological systems, it is important to achieve a deep understanding of the factors affecting coating stability and behavior. We have designed a system that tests the coating stability of amphiphilic polymers through a simple fluorescent readout using either polarity sensing ESIPT (excited state intramolecular proton transfer) dyes or NP FRET (Förster resonance energy transfer). The stability of the coating was determined in response to changes in polarity, pH and ionic strength in the medium. Using the ESIPT system we observed linear changes in signal up to ∼20-25% v/v of co-solvent addition, constituting a break point. Based on such data, we propose a model for coating instability and the important adjustable parameters, such as the electrical charge distribution. FRET data provided confirmatory evidence for the model. The ESIPT dyes and FRET based methods represent new, simple tools for testing NP coating stability in complex environments. © 2017 The Royal Society of Chemistry.
format JOUR
author Azcárate, J.C.
Díaz, S.A.
Fauerbach, J.A.
Gillanders, F.
Rubert, A.A.
Jares-Erijman, E.A.
Jovin, T.M.
Fonticelli, M.H.
author_facet Azcárate, J.C.
Díaz, S.A.
Fauerbach, J.A.
Gillanders, F.
Rubert, A.A.
Jares-Erijman, E.A.
Jovin, T.M.
Fonticelli, M.H.
author_sort Azcárate, J.C.
title ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_short ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_fullStr ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_full_unstemmed ESIPT and FRET probes for monitoring nanoparticle polymer coating stability
title_sort esipt and fret probes for monitoring nanoparticle polymer coating stability
url http://hdl.handle.net/20.500.12110/paper_20403364_v9_n25_p8647_Azcarate
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