Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season

Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genoty...

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Detalles Bibliográficos
Autores principales: Yerkovich, N., Palazzini, J.M., Sulyok, M., Chulze, S.N.
Formato: JOUR
Materias:
Chemotype
Fusarium
Genotype
Mycotoxins
Triticum aestivum L
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19825676_v42_n3_p190_Yerkovich
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_19825676_v42_n3_p190_Yerkovich
record_format dspace
spelling todo:paper_19825676_v42_n3_p190_Yerkovich2023-10-03T16:37:25Z Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season Yerkovich, N. Palazzini, J.M. Sulyok, M. Chulze, S.N. Chemotype Fusarium Genotype Mycotoxins Triticum aestivum L Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina. © 2017, Sociedade Brasileira de Fitopatologia. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19825676_v42_n3_p190_Yerkovich
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Chemotype
Fusarium
Genotype
Mycotoxins
Triticum aestivum L
spellingShingle Chemotype
Fusarium
Genotype
Mycotoxins
Triticum aestivum L
Yerkovich, N.
Palazzini, J.M.
Sulyok, M.
Chulze, S.N.
Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
topic_facet Chemotype
Fusarium
Genotype
Mycotoxins
Triticum aestivum L
description Members of Fusarium graminearum species complex are the main pathogens associated with Fusarium head blight (FHB) in wheat in Argentina, which produce trichothecene mycotoxins that are found in wheat grain and by-products. The aim of this study was to determine the chemotype and trichothecene genotypes of Fusarium graminearum species complex strains isolated from wheat in Argentina during an epidemic and a non-epidemic harvest season. A total of 115 and 108 strains obtained from wheat during 2012/13 and 2014/15 harvest seasons, respectively, were identified as belonging to the Fusarium graminearum species complex. PCR assays were used to differentiate the 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON) and nivalenol (NIV) genotypes. The trichothecene type B chemotype, zearalenone, and NX-2 toxin profiles were determined based on chromatographic (LC-MS/MS) analysis. Differences in the genotype frequency were due to the target locus analyzed and year of isolation of the strain. The most common genotype and chemotype was 15-ADON in both seasons, but an increasing frequency of the 3-ADON chemotype was found in the non-epidemic season (2014/15). The strains were able to produce zearalenone and its masked derivatives, but not the type A trichothecene NX-2. This is the first report of the production of zearalenone and its modified mycotoxins by Fusarium graminearum species complex strains isolated from Argentina. © 2017, Sociedade Brasileira de Fitopatologia.
format JOUR
author Yerkovich, N.
Palazzini, J.M.
Sulyok, M.
Chulze, S.N.
author_facet Yerkovich, N.
Palazzini, J.M.
Sulyok, M.
Chulze, S.N.
author_sort Yerkovich, N.
title Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_short Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_full Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_fullStr Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_full_unstemmed Trichothecene genotypes, chemotypes and zearalenone production by Fusarium graminearum species complex strains causing Fusarium head blight in Argentina during an epidemic and non-epidemic season
title_sort trichothecene genotypes, chemotypes and zearalenone production by fusarium graminearum species complex strains causing fusarium head blight in argentina during an epidemic and non-epidemic season
url http://hdl.handle.net/20.500.12110/paper_19825676_v42_n3_p190_Yerkovich
work_keys_str_mv AT yerkovichn trichothecenegenotypeschemotypesandzearalenoneproductionbyfusariumgraminearumspeciescomplexstrainscausingfusariumheadblightinargentinaduringanepidemicandnonepidemicseason
AT palazzinijm trichothecenegenotypeschemotypesandzearalenoneproductionbyfusariumgraminearumspeciescomplexstrainscausingfusariumheadblightinargentinaduringanepidemicandnonepidemicseason
AT sulyokm trichothecenegenotypeschemotypesandzearalenoneproductionbyfusariumgraminearumspeciescomplexstrainscausingfusariumheadblightinargentinaduringanepidemicandnonepidemicseason
AT chulzesn trichothecenegenotypeschemotypesandzearalenoneproductionbyfusariumgraminearumspeciescomplexstrainscausingfusariumheadblightinargentinaduringanepidemicandnonepidemicseason
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