Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication

Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternati...

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Autores principales:	Soler-Arango, J., Xaubet, M., Giuliani, L., Grondona, D., Brelles-Mariño, G.
Formato:	JOUR
Materias:	Air-based plasma Biofilm eradication Biofilms Non-thermal plasmas Pseudomonas aeruginosa Pseudomonas biofilms Sterilization Airports Bacteria Dielectric devices Dielectric materials Flow control Military bases Plasma applications Plasma sources Plasma theory Sterilization (cleaning) Surface treatment Continuous culture Decimal reduction Dielectric barrier discharge plasmas Gas-discharge plasmas Nonthermal plasma Nosocomial infection Planktonic bacteria Electric discharges carbon cell DNA deoxyribonuclease glass polycarbonate stainless steel air airflow ambient air Article bacterial viability biofilm carbon source cell density cell survival chemical composition colony forming unit continuous culture controlled study culture medium electric potential electrode epifluorescence microscopy hydrophilicity hydrophobicity nonhuman pH physical chemistry priority journal reduction (chemistry) static electricity surface property surface tension survival rate temperature sensitivity waveform
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19475764_v7_n1_p43_SolerArango
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_19475764_v7_n1_p43_SolerArango
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spelling	todo:paper_19475764_v7_n1_p43_SolerArango2023-10-03T16:37:08Z Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication Soler-Arango, J. Xaubet, M. Giuliani, L. Grondona, D. Brelles-Mariño, G. Air-based plasma Biofilm eradication Biofilms Non-thermal plasmas Pseudomonas aeruginosa Pseudomonas biofilms Sterilization Airports Bacteria Biofilms Dielectric devices Dielectric materials Flow control Military bases Plasma applications Plasma sources Plasma theory Sterilization (cleaning) Surface treatment Continuous culture Decimal reduction Dielectric barrier discharge plasmas Gas-discharge plasmas Nonthermal plasma Nosocomial infection Planktonic bacteria Pseudomonas aeruginosa Electric discharges carbon cell DNA deoxyribonuclease glass polycarbonate stainless steel air airflow ambient air Article bacterial viability biofilm carbon source cell density cell survival chemical composition colony forming unit continuous culture controlled study culture medium electric potential electrode epifluorescence microscopy hydrophilicity hydrophobicity nonhuman pH physical chemistry priority journal Pseudomonas aeruginosa reduction (chemistry) static electricity surface property surface tension survival rate temperature sensitivity waveform Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternative because plasmas contain a mixture of reactive agents that are well known for bacterial decontamination. This study assesses culture medium-abiotic surface combinations leading to robust biofilms and tests an air-based coaxial dielectric barrier discharge (DBD) plasma source on Pseudomonas aeruginosa biofilms grown in continuous culture under those selected conditions. Biofilms were eradicated after a 15-min plasma treatment, resulting in a CFU/mL decrease of 5.6 log10 units. CFU/mL decreases of 1.6 and 2.7 log10 units were achieved after a 3-min plasma exposure to ambient and moistened air plasma, respectively, although viability assays showed that some cells were alive. Moistened-air plasma resulted in a faster biofilm inactivation, with decimal reduction times of 1.14 and 4.36 min. The coaxial DBD air-based plasma source presented here is effective for Pseudomonas biofilm inactivation, affordable because it does not rely on expensive gases, and easy to handle for indirect surface treatment. To the best of our knowledge, the search for the best combination medium surface leading to robust biofilms before plasma treatment has not been previously assessed. © 2017 by Begell House, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19475764_v7_n1_p43_SolerArango
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Air-based plasma Biofilm eradication Biofilms Non-thermal plasmas Pseudomonas aeruginosa Pseudomonas biofilms Sterilization Airports Bacteria Biofilms Dielectric devices Dielectric materials Flow control Military bases Plasma applications Plasma sources Plasma theory Sterilization (cleaning) Surface treatment Continuous culture Decimal reduction Dielectric barrier discharge plasmas Gas-discharge plasmas Nonthermal plasma Nosocomial infection Planktonic bacteria Pseudomonas aeruginosa Electric discharges carbon cell DNA deoxyribonuclease glass polycarbonate stainless steel air airflow ambient air Article bacterial viability biofilm carbon source cell density cell survival chemical composition colony forming unit continuous culture controlled study culture medium electric potential electrode epifluorescence microscopy hydrophilicity hydrophobicity nonhuman pH physical chemistry priority journal Pseudomonas aeruginosa reduction (chemistry) static electricity surface property surface tension survival rate temperature sensitivity waveform
spellingShingle	Air-based plasma Biofilm eradication Biofilms Non-thermal plasmas Pseudomonas aeruginosa Pseudomonas biofilms Sterilization Airports Bacteria Biofilms Dielectric devices Dielectric materials Flow control Military bases Plasma applications Plasma sources Plasma theory Sterilization (cleaning) Surface treatment Continuous culture Decimal reduction Dielectric barrier discharge plasmas Gas-discharge plasmas Nonthermal plasma Nosocomial infection Planktonic bacteria Pseudomonas aeruginosa Electric discharges carbon cell DNA deoxyribonuclease glass polycarbonate stainless steel air airflow ambient air Article bacterial viability biofilm carbon source cell density cell survival chemical composition colony forming unit continuous culture controlled study culture medium electric potential electrode epifluorescence microscopy hydrophilicity hydrophobicity nonhuman pH physical chemistry priority journal Pseudomonas aeruginosa reduction (chemistry) static electricity surface property surface tension survival rate temperature sensitivity waveform Soler-Arango, J. Xaubet, M. Giuliani, L. Grondona, D. Brelles-Mariño, G. Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
topic_facet	Air-based plasma Biofilm eradication Biofilms Non-thermal plasmas Pseudomonas aeruginosa Pseudomonas biofilms Sterilization Airports Bacteria Biofilms Dielectric devices Dielectric materials Flow control Military bases Plasma applications Plasma sources Plasma theory Sterilization (cleaning) Surface treatment Continuous culture Decimal reduction Dielectric barrier discharge plasmas Gas-discharge plasmas Nonthermal plasma Nosocomial infection Planktonic bacteria Pseudomonas aeruginosa Electric discharges carbon cell DNA deoxyribonuclease glass polycarbonate stainless steel air airflow ambient air Article bacterial viability biofilm carbon source cell density cell survival chemical composition colony forming unit continuous culture controlled study culture medium electric potential electrode epifluorescence microscopy hydrophilicity hydrophobicity nonhuman pH physical chemistry priority journal Pseudomonas aeruginosa reduction (chemistry) static electricity surface property surface tension survival rate temperature sensitivity waveform
description	Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternative because plasmas contain a mixture of reactive agents that are well known for bacterial decontamination. This study assesses culture medium-abiotic surface combinations leading to robust biofilms and tests an air-based coaxial dielectric barrier discharge (DBD) plasma source on Pseudomonas aeruginosa biofilms grown in continuous culture under those selected conditions. Biofilms were eradicated after a 15-min plasma treatment, resulting in a CFU/mL decrease of 5.6 log10 units. CFU/mL decreases of 1.6 and 2.7 log10 units were achieved after a 3-min plasma exposure to ambient and moistened air plasma, respectively, although viability assays showed that some cells were alive. Moistened-air plasma resulted in a faster biofilm inactivation, with decimal reduction times of 1.14 and 4.36 min. The coaxial DBD air-based plasma source presented here is effective for Pseudomonas biofilm inactivation, affordable because it does not rely on expensive gases, and easy to handle for indirect surface treatment. To the best of our knowledge, the search for the best combination medium surface leading to robust biofilms before plasma treatment has not been previously assessed. © 2017 by Begell House, Inc.
format	JOUR
author	Soler-Arango, J. Xaubet, M. Giuliani, L. Grondona, D. Brelles-Mariño, G.
author_facet	Soler-Arango, J. Xaubet, M. Giuliani, L. Grondona, D. Brelles-Mariño, G.
author_sort	Soler-Arango, J.
title	Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
title_short	Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
title_full	Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
title_fullStr	Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
title_full_unstemmed	Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication
title_sort	air-based coaxial dielectric barrier discharge plasma source for pseudomonas aeruginosa biofilm eradication
url	http://hdl.handle.net/20.500.12110/paper_19475764_v7_n1_p43_SolerArango
work_keys_str_mv	AT solerarangoj airbasedcoaxialdielectricbarrierdischargeplasmasourceforpseudomonasaeruginosabiofilmeradication AT xaubetm airbasedcoaxialdielectricbarrierdischargeplasmasourceforpseudomonasaeruginosabiofilmeradication AT giulianil airbasedcoaxialdielectricbarrierdischargeplasmasourceforpseudomonasaeruginosabiofilmeradication AT grondonad airbasedcoaxialdielectricbarrierdischargeplasmasourceforpseudomonasaeruginosabiofilmeradication AT brellesmarinog airbasedcoaxialdielectricbarrierdischargeplasmasourceforpseudomonasaeruginosabiofilmeradication
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Air-based coaxial dielectric barrier discharge plasma source for Pseudomonas aeruginosa biofilm eradication

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