Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca 2+ -liberating secondmessenger inositol trisphosphate (IP 3 ) diffuses with a coefficient (∼280 mm2 s -1 ) similar to...
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19450877_v9_n453_p_Dickinson |
| Aporte de: |
| id |
todo:paper_19450877_v9_n453_p_Dickinson |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_19450877_v9_n453_p_Dickinson2023-10-03T16:37:06Z Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action Dickinson, G.D. Ellefsen, K.L. Dawson, S.P. Pearson, J.E. Parker, I. calcium ion inositol trisphosphate calcium inositol 1,4,5 trisphosphate Article binding site cell function controlled study COS 7 cell line cytoplasm cytosol degradation dendrite diffusion coefficient dissociation constant gap junction human human cell neuroblastoma cell oocyte photolysis photon priority journal calcium signaling cytoplasm metabolism neuroblastoma tumor cell line Calcium Calcium Signaling Cell Line, Tumor Cytoplasm Humans Inositol 1,4,5-Trisphosphate Neuroblastoma The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca 2+ -liberating secondmessenger inositol trisphosphate (IP 3 ) diffuses with a coefficient (∼280 mm2 s -1 ) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP 3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP 3 -evoked Ca 2+ puffs to monitor IP 3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm 2 s -1 ) about 30-fold slower than that previously reported. We propose that diffusion of IP 3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP 3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP 3 should better be considered as a local rather than a global cellular messenger. 2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19450877_v9_n453_p_Dickinson |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
calcium ion inositol trisphosphate calcium inositol 1,4,5 trisphosphate Article binding site cell function controlled study COS 7 cell line cytoplasm cytosol degradation dendrite diffusion coefficient dissociation constant gap junction human human cell neuroblastoma cell oocyte photolysis photon priority journal calcium signaling cytoplasm metabolism neuroblastoma tumor cell line Calcium Calcium Signaling Cell Line, Tumor Cytoplasm Humans Inositol 1,4,5-Trisphosphate Neuroblastoma |
| spellingShingle |
calcium ion inositol trisphosphate calcium inositol 1,4,5 trisphosphate Article binding site cell function controlled study COS 7 cell line cytoplasm cytosol degradation dendrite diffusion coefficient dissociation constant gap junction human human cell neuroblastoma cell oocyte photolysis photon priority journal calcium signaling cytoplasm metabolism neuroblastoma tumor cell line Calcium Calcium Signaling Cell Line, Tumor Cytoplasm Humans Inositol 1,4,5-Trisphosphate Neuroblastoma Dickinson, G.D. Ellefsen, K.L. Dawson, S.P. Pearson, J.E. Parker, I. Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| topic_facet |
calcium ion inositol trisphosphate calcium inositol 1,4,5 trisphosphate Article binding site cell function controlled study COS 7 cell line cytoplasm cytosol degradation dendrite diffusion coefficient dissociation constant gap junction human human cell neuroblastoma cell oocyte photolysis photon priority journal calcium signaling cytoplasm metabolism neuroblastoma tumor cell line Calcium Calcium Signaling Cell Line, Tumor Cytoplasm Humans Inositol 1,4,5-Trisphosphate Neuroblastoma |
| description |
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca 2+ -liberating secondmessenger inositol trisphosphate (IP 3 ) diffuses with a coefficient (∼280 mm2 s -1 ) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP 3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP 3 -evoked Ca 2+ puffs to monitor IP 3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm 2 s -1 ) about 30-fold slower than that previously reported. We propose that diffusion of IP 3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP 3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP 3 should better be considered as a local rather than a global cellular messenger. 2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. |
| format |
JOUR |
| author |
Dickinson, G.D. Ellefsen, K.L. Dawson, S.P. Pearson, J.E. Parker, I. |
| author_facet |
Dickinson, G.D. Ellefsen, K.L. Dawson, S.P. Pearson, J.E. Parker, I. |
| author_sort |
Dickinson, G.D. |
| title |
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| title_short |
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| title_full |
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| title_fullStr |
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| title_full_unstemmed |
Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| title_sort |
hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action |
| url |
http://hdl.handle.net/20.500.12110/paper_19450877_v9_n453_p_Dickinson |
| work_keys_str_mv |
AT dickinsongd hinderedcytoplasmicdiffusionofinositoltrisphosphaterestrictsitscellularrangeofaction AT ellefsenkl hinderedcytoplasmicdiffusionofinositoltrisphosphaterestrictsitscellularrangeofaction AT dawsonsp hinderedcytoplasmicdiffusionofinositoltrisphosphaterestrictsitscellularrangeofaction AT pearsonje hinderedcytoplasmicdiffusionofinositoltrisphosphaterestrictsitscellularrangeofaction AT parkeri hinderedcytoplasmicdiffusionofinositoltrisphosphaterestrictsitscellularrangeofaction |
| _version_ |
1807323256410079232 |