Survey of argentinean human plasma for ochratoxin a
The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires provinc...
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todo:paper_19440049_v25_n5_p635_Pacin2023-10-03T16:36:58Z Survey of argentinean human plasma for ochratoxin a Pacin, A.M. Ciancio Bovier, E.V. Motta, E. Resnik, S.L. Villa, D. Olsen, M. Exposure Human plasma Mycotoxin Ochratoxin ochratoxin ester derivative ochratoxin adult antibody affinity Argentina article blood donor blood sampling controlled study female food contamination health survey high performance liquid chromatography human male priority journal solid phase extraction South and Central America blood analysis immunoaffinity chromatography major clinical study Adult Agriculture Argentina Chromatography, Liquid Female Food Contamination Humans Male Ochratoxins Statistics as Topic The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires province. OTA was determined by high-performance liquid chromatography (HPLC) in 199 plasma samples from blood donors in Mar del Plata and 236 from General Rodríguez. Solid-phase extraction with Bakerbond® C-18 cartridge and a final purification with Ochraprep® immunoaffinity columns was employed. The limit of quantification of ochratoxin A was 0.019ngml−1 and the confirmation of OTA was by formation of ochratoxin A methyl ester. The results showed that 63.8% of human plasma samples from Mar del Plata and 62.3% from General Rodríguez were positive for OTA, with Winsorized means of 0.15 and 0.43ngml−1, respectively. It is important to continue the research to detect the foods responsible of the presence of OTA in plasma. © 2008 Taylor & Francis Group, LLC. Fil:Motta, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnik, S.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19440049_v25_n5_p635_Pacin |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Exposure Human plasma Mycotoxin Ochratoxin ochratoxin ester derivative ochratoxin adult antibody affinity Argentina article blood donor blood sampling controlled study female food contamination health survey high performance liquid chromatography human male priority journal solid phase extraction South and Central America blood analysis immunoaffinity chromatography major clinical study Adult Agriculture Argentina Chromatography, Liquid Female Food Contamination Humans Male Ochratoxins Statistics as Topic |
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Exposure Human plasma Mycotoxin Ochratoxin ochratoxin ester derivative ochratoxin adult antibody affinity Argentina article blood donor blood sampling controlled study female food contamination health survey high performance liquid chromatography human male priority journal solid phase extraction South and Central America blood analysis immunoaffinity chromatography major clinical study Adult Agriculture Argentina Chromatography, Liquid Female Food Contamination Humans Male Ochratoxins Statistics as Topic Pacin, A.M. Ciancio Bovier, E.V. Motta, E. Resnik, S.L. Villa, D. Olsen, M. Survey of argentinean human plasma for ochratoxin a |
| topic_facet |
Exposure Human plasma Mycotoxin Ochratoxin ochratoxin ester derivative ochratoxin adult antibody affinity Argentina article blood donor blood sampling controlled study female food contamination health survey high performance liquid chromatography human male priority journal solid phase extraction South and Central America blood analysis immunoaffinity chromatography major clinical study Adult Agriculture Argentina Chromatography, Liquid Female Food Contamination Humans Male Ochratoxins Statistics as Topic |
| description |
The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires province. OTA was determined by high-performance liquid chromatography (HPLC) in 199 plasma samples from blood donors in Mar del Plata and 236 from General Rodríguez. Solid-phase extraction with Bakerbond® C-18 cartridge and a final purification with Ochraprep® immunoaffinity columns was employed. The limit of quantification of ochratoxin A was 0.019ngml−1 and the confirmation of OTA was by formation of ochratoxin A methyl ester. The results showed that 63.8% of human plasma samples from Mar del Plata and 62.3% from General Rodríguez were positive for OTA, with Winsorized means of 0.15 and 0.43ngml−1, respectively. It is important to continue the research to detect the foods responsible of the presence of OTA in plasma. © 2008 Taylor & Francis Group, LLC. |
| format |
JOUR |
| author |
Pacin, A.M. Ciancio Bovier, E.V. Motta, E. Resnik, S.L. Villa, D. Olsen, M. |
| author_facet |
Pacin, A.M. Ciancio Bovier, E.V. Motta, E. Resnik, S.L. Villa, D. Olsen, M. |
| author_sort |
Pacin, A.M. |
| title |
Survey of argentinean human plasma for ochratoxin a |
| title_short |
Survey of argentinean human plasma for ochratoxin a |
| title_full |
Survey of argentinean human plasma for ochratoxin a |
| title_fullStr |
Survey of argentinean human plasma for ochratoxin a |
| title_full_unstemmed |
Survey of argentinean human plasma for ochratoxin a |
| title_sort |
survey of argentinean human plasma for ochratoxin a |
| url |
http://hdl.handle.net/20.500.12110/paper_19440049_v25_n5_p635_Pacin |
| work_keys_str_mv |
AT pacinam surveyofargentineanhumanplasmaforochratoxina AT cianciobovierev surveyofargentineanhumanplasmaforochratoxina AT mottae surveyofargentineanhumanplasmaforochratoxina AT resniksl surveyofargentineanhumanplasmaforochratoxina AT villad surveyofargentineanhumanplasmaforochratoxina AT olsenm surveyofargentineanhumanplasmaforochratoxina |
| _version_ |
1807314915189325824 |