Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri

A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10...

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Autores principales: Fazio, A.T., Adler, M.T., Maier, M.S.
Formato: JOUR
Materias:
Axenic cultures
Lichen metabolites
Ramalina celastri
Triacylglycerides
Usnic acid
Benzofurans
Lichens
Symbiosis
Triglycerides
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1934578X_v9_n2_p213_Fazio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_1934578X_v9_n2_p213_Fazio2023-10-03T16:36:15Z Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri Fazio, A.T. Adler, M.T. Maier, M.S. Axenic cultures Lichen metabolites Ramalina celastri Triacylglycerides Usnic acid Benzofurans Lichens Symbiosis Triglycerides A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory. Fil:Fazio, A.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Adler, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maier, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1934578X_v9_n2_p213_Fazio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Axenic cultures
Lichen metabolites
Ramalina celastri
Triacylglycerides
Usnic acid
Benzofurans
Lichens
Symbiosis
Triglycerides
spellingShingle Axenic cultures
Lichen metabolites
Ramalina celastri
Triacylglycerides
Usnic acid
Benzofurans
Lichens
Symbiosis
Triglycerides
Fazio, A.T.
Adler, M.T.
Maier, M.S.
Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
topic_facet Axenic cultures
Lichen metabolites
Ramalina celastri
Triacylglycerides
Usnic acid
Benzofurans
Lichens
Symbiosis
Triglycerides
description A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.
format JOUR
author Fazio, A.T.
Adler, M.T.
Maier, M.S.
author_facet Fazio, A.T.
Adler, M.T.
Maier, M.S.
author_sort Fazio, A.T.
title Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
title_short Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
title_full Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
title_fullStr Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
title_full_unstemmed Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
title_sort usnic acid and triacylglycerides production by the cultured lichen mycobiont of ramalina celastri
url http://hdl.handle.net/20.500.12110/paper_1934578X_v9_n2_p213_Fazio
work_keys_str_mv AT fazioat usnicacidandtriacylglyceridesproductionbytheculturedlichenmycobiontoframalinacelastri
AT adlermt usnicacidandtriacylglyceridesproductionbytheculturedlichenmycobiontoframalinacelastri
AT maierms usnicacidandtriacylglyceridesproductionbytheculturedlichenmycobiontoframalinacelastri
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