Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species
Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and musc...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_19343639_v2016_n_p_MestreCitrinovitz |
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todo:paper_19343639_v2016_n_p_MestreCitrinovitz2023-10-03T16:36:13Z Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species Mestre-Citrinovitz, A.C. Sestelo, A.J. Ceballos, M.B. Barañao, J.L. Saragüeta, P. Biobank Cell culture Panthera onca animal cell animal tissue Article biobank biome blood storage Brazil cell culture cell culture technique cell isolation cryopreservation ear cartilage endangered species fibroblast genetic resource hair cell jaguar muscle biopsy muscle tissue nonhuman ovary tissue priority journal skin fibroblast somatic cell South American sperm preservation testis tissue wildlife conservation animal cartilage cell separation cytology fibroblast metabolism muscle Panthera procedures skin Animals Cartilage Cell Culture Techniques Cell Separation Cells, Cultured Cryopreservation Endangered Species Fibroblasts Muscles Panthera Skin Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19343639_v2016_n_p_MestreCitrinovitz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biobank Cell culture Panthera onca animal cell animal tissue Article biobank biome blood storage Brazil cell culture cell culture technique cell isolation cryopreservation ear cartilage endangered species fibroblast genetic resource hair cell jaguar muscle biopsy muscle tissue nonhuman ovary tissue priority journal skin fibroblast somatic cell South American sperm preservation testis tissue wildlife conservation animal cartilage cell separation cytology fibroblast metabolism muscle Panthera procedures skin Animals Cartilage Cell Culture Techniques Cell Separation Cells, Cultured Cryopreservation Endangered Species Fibroblasts Muscles Panthera Skin |
| spellingShingle |
Biobank Cell culture Panthera onca animal cell animal tissue Article biobank biome blood storage Brazil cell culture cell culture technique cell isolation cryopreservation ear cartilage endangered species fibroblast genetic resource hair cell jaguar muscle biopsy muscle tissue nonhuman ovary tissue priority journal skin fibroblast somatic cell South American sperm preservation testis tissue wildlife conservation animal cartilage cell separation cytology fibroblast metabolism muscle Panthera procedures skin Animals Cartilage Cell Culture Techniques Cell Separation Cells, Cultured Cryopreservation Endangered Species Fibroblasts Muscles Panthera Skin Mestre-Citrinovitz, A.C. Sestelo, A.J. Ceballos, M.B. Barañao, J.L. Saragüeta, P. Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| topic_facet |
Biobank Cell culture Panthera onca animal cell animal tissue Article biobank biome blood storage Brazil cell culture cell culture technique cell isolation cryopreservation ear cartilage endangered species fibroblast genetic resource hair cell jaguar muscle biopsy muscle tissue nonhuman ovary tissue priority journal skin fibroblast somatic cell South American sperm preservation testis tissue wildlife conservation animal cartilage cell separation cytology fibroblast metabolism muscle Panthera procedures skin Animals Cartilage Cell Culture Techniques Cell Separation Cells, Cultured Cryopreservation Endangered Species Fibroblasts Muscles Panthera Skin |
| description |
Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc. |
| format |
JOUR |
| author |
Mestre-Citrinovitz, A.C. Sestelo, A.J. Ceballos, M.B. Barañao, J.L. Saragüeta, P. |
| author_facet |
Mestre-Citrinovitz, A.C. Sestelo, A.J. Ceballos, M.B. Barañao, J.L. Saragüeta, P. |
| author_sort |
Mestre-Citrinovitz, A.C. |
| title |
Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| title_short |
Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| title_full |
Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| title_fullStr |
Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| title_full_unstemmed |
Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species |
| title_sort |
isolation of primary fibroblast culture from wildlife: the panthera onca case to preserve a south american endangered species |
| url |
http://hdl.handle.net/20.500.12110/paper_19343639_v2016_n_p_MestreCitrinovitz |
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