The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence
The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and...
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todo:paper_19326203_v8_n11_p_RuizRanwez2023-10-03T16:35:29Z The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence Ruiz-Ranwez, V. Posadas, D.M. Estein, S.M. Abdian, P.L. Martin, F.A. Zorreguieta, A. bacterial protein protein BtaF unclassified drug abiotic stress animal cell animal experiment animal model antibiotic resistance antibody response article bacterial virulence Brucella suis brucellosis cell adhesion cell division controlled study extracellular matrix female gene locus human human cell life cycle mouse nonhuman polymerase chain reaction population dynamics protein aggregation protein analysis protein expression protein function protein localization signal transduction Adhesins, Bacterial Animals Bacterial Adhesion Brucella suis Brucellosis Cell Line Extracellular Matrix Humans Male Mice Multigene Family Protein Multimerization Protein Transport Swine Virulence The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle. © 2013 Ruiz-Ranwez et al. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abdian, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martin, F.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_RuizRanwez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial protein protein BtaF unclassified drug abiotic stress animal cell animal experiment animal model antibiotic resistance antibody response article bacterial virulence Brucella suis brucellosis cell adhesion cell division controlled study extracellular matrix female gene locus human human cell life cycle mouse nonhuman polymerase chain reaction population dynamics protein aggregation protein analysis protein expression protein function protein localization signal transduction Adhesins, Bacterial Animals Bacterial Adhesion Brucella suis Brucellosis Cell Line Extracellular Matrix Humans Male Mice Multigene Family Protein Multimerization Protein Transport Swine Virulence |
| spellingShingle |
bacterial protein protein BtaF unclassified drug abiotic stress animal cell animal experiment animal model antibiotic resistance antibody response article bacterial virulence Brucella suis brucellosis cell adhesion cell division controlled study extracellular matrix female gene locus human human cell life cycle mouse nonhuman polymerase chain reaction population dynamics protein aggregation protein analysis protein expression protein function protein localization signal transduction Adhesins, Bacterial Animals Bacterial Adhesion Brucella suis Brucellosis Cell Line Extracellular Matrix Humans Male Mice Multigene Family Protein Multimerization Protein Transport Swine Virulence Ruiz-Ranwez, V. Posadas, D.M. Estein, S.M. Abdian, P.L. Martin, F.A. Zorreguieta, A. The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| topic_facet |
bacterial protein protein BtaF unclassified drug abiotic stress animal cell animal experiment animal model antibiotic resistance antibody response article bacterial virulence Brucella suis brucellosis cell adhesion cell division controlled study extracellular matrix female gene locus human human cell life cycle mouse nonhuman polymerase chain reaction population dynamics protein aggregation protein analysis protein expression protein function protein localization signal transduction Adhesins, Bacterial Animals Bacterial Adhesion Brucella suis Brucellosis Cell Line Extracellular Matrix Humans Male Mice Multigene Family Protein Multimerization Protein Transport Swine Virulence |
| description |
The adhesion of bacterial pathogens to host cells is an event that determines infection, and ultimately invasion and intracellular multiplication. Several evidences have recently shown that this rule is also truth for the intracellular pathogen Brucella. Brucella suis displays the unipolar BmaC and BtaE adhesins, which belong to the monomeric and trimeric autotransporter (TA) families, respectively. It was previously shown that these adhesins are involved in bacterial adhesion to host cells and components of the extracellular matrix (ECM). In this work we describe the role of a new member of the TA family of B. suis (named BtaF) in the adhesive properties of the bacterial surface. BtaF conferred the bacteria that carried it a promiscuous adhesiveness to various ECM components and the ability to attach to an abiotic surface. Furthermore, BtaF was found to participate in bacterial adhesion to epithelial cells and was required for full virulence in mice. Similar to BmaC and BtaE, the BtaF adhesin was expressed in a small subpopulation of bacteria, and in all cases, it was detected at the new pole generated after cell division. Interestingly, BtaF was also implicated in the resistance of B. suis to porcine serum. Our findings emphasize the impact of TAs in the Brucella lifecycle. © 2013 Ruiz-Ranwez et al. |
| format |
JOUR |
| author |
Ruiz-Ranwez, V. Posadas, D.M. Estein, S.M. Abdian, P.L. Martin, F.A. Zorreguieta, A. |
| author_facet |
Ruiz-Ranwez, V. Posadas, D.M. Estein, S.M. Abdian, P.L. Martin, F.A. Zorreguieta, A. |
| author_sort |
Ruiz-Ranwez, V. |
| title |
The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| title_short |
The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| title_full |
The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| title_fullStr |
The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| title_full_unstemmed |
The BtaF trimeric autotransporter of Brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| title_sort |
btaf trimeric autotransporter of brucella suis is involved in attachment to various surfaces, resistance to serum and virulence |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_RuizRanwez |
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