EDA-containing fibronectin increases proliferation of embryonic stem cells
Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC's proliferation rate. The mitogenic prope...
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todo:paper_19326203_v8_n11_p_Losino2023-10-03T16:35:29Z EDA-containing fibronectin increases proliferation of embryonic stem cells Losino, N. Waisman, A. Solari, C. Luzzani, C. Espinosa, D.F. Sassone, A. Muro, A.F. Miriuka, S. Sevlever, G. Barañao, L. Guberman, A. culture medium extra domain A fibronectin, human fibronectin animal cell culture cell proliferation culture medium cytology drug effects embryonic stem cell fibroblast genetics human metabolism mouse mutant mouse strain pharmacology Animals Cell Proliferation Cells, Cultured Culture Media, Conditioned Embryonic Stem Cells Fibroblasts Fibronectins Humans Mice Mice, Mutant Strains Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC's proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA +). Here, we investigated if the FN EDA+ isoform increased proliferation of mouse and human ES cells. We analyzed cell proliferation using conditioned media produced by different mouse embryonic fibroblast (MEF) lines genetically engineered to express FN constitutively including or excluding the EDA domain (FN EDA-), and in media supplemented with recombinant peptides containing or not the EDA. We found that the presence of EDA in the medium increased mouse and human ESC's proliferation rate. Here we showed for the first time that this FN isoform enhances ESC's proliferation. These findings suggest a possible conserved behavior for regulation of ES cells proliferation by this FN isoform and could contribute to improve their culturing conditions both for research and cell therapy. © 2013 Losino et al. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sassone, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_Losino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
culture medium extra domain A fibronectin, human fibronectin animal cell culture cell proliferation culture medium cytology drug effects embryonic stem cell fibroblast genetics human metabolism mouse mutant mouse strain pharmacology Animals Cell Proliferation Cells, Cultured Culture Media, Conditioned Embryonic Stem Cells Fibroblasts Fibronectins Humans Mice Mice, Mutant Strains |
| spellingShingle |
culture medium extra domain A fibronectin, human fibronectin animal cell culture cell proliferation culture medium cytology drug effects embryonic stem cell fibroblast genetics human metabolism mouse mutant mouse strain pharmacology Animals Cell Proliferation Cells, Cultured Culture Media, Conditioned Embryonic Stem Cells Fibroblasts Fibronectins Humans Mice Mice, Mutant Strains Losino, N. Waisman, A. Solari, C. Luzzani, C. Espinosa, D.F. Sassone, A. Muro, A.F. Miriuka, S. Sevlever, G. Barañao, L. Guberman, A. EDA-containing fibronectin increases proliferation of embryonic stem cells |
| topic_facet |
culture medium extra domain A fibronectin, human fibronectin animal cell culture cell proliferation culture medium cytology drug effects embryonic stem cell fibroblast genetics human metabolism mouse mutant mouse strain pharmacology Animals Cell Proliferation Cells, Cultured Culture Media, Conditioned Embryonic Stem Cells Fibroblasts Fibronectins Humans Mice Mice, Mutant Strains |
| description |
Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC's proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA +). Here, we investigated if the FN EDA+ isoform increased proliferation of mouse and human ES cells. We analyzed cell proliferation using conditioned media produced by different mouse embryonic fibroblast (MEF) lines genetically engineered to express FN constitutively including or excluding the EDA domain (FN EDA-), and in media supplemented with recombinant peptides containing or not the EDA. We found that the presence of EDA in the medium increased mouse and human ESC's proliferation rate. Here we showed for the first time that this FN isoform enhances ESC's proliferation. These findings suggest a possible conserved behavior for regulation of ES cells proliferation by this FN isoform and could contribute to improve their culturing conditions both for research and cell therapy. © 2013 Losino et al. |
| format |
JOUR |
| author |
Losino, N. Waisman, A. Solari, C. Luzzani, C. Espinosa, D.F. Sassone, A. Muro, A.F. Miriuka, S. Sevlever, G. Barañao, L. Guberman, A. |
| author_facet |
Losino, N. Waisman, A. Solari, C. Luzzani, C. Espinosa, D.F. Sassone, A. Muro, A.F. Miriuka, S. Sevlever, G. Barañao, L. Guberman, A. |
| author_sort |
Losino, N. |
| title |
EDA-containing fibronectin increases proliferation of embryonic stem cells |
| title_short |
EDA-containing fibronectin increases proliferation of embryonic stem cells |
| title_full |
EDA-containing fibronectin increases proliferation of embryonic stem cells |
| title_fullStr |
EDA-containing fibronectin increases proliferation of embryonic stem cells |
| title_full_unstemmed |
EDA-containing fibronectin increases proliferation of embryonic stem cells |
| title_sort |
eda-containing fibronectin increases proliferation of embryonic stem cells |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n11_p_Losino |
| work_keys_str_mv |
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1807322899115147264 |