The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae

Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae he...

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Autores principales:	Barraza, C.E., Solari, C.A., Marcovich, I., Kershaw, C., Galello, F., Rossi, S., Ashe, M.P., Portela, P.
Formato:	JOUR
Materias:	cyclic AMP dependent protein kinase heat shock protein 30 initiation factor 4E initiation factor 4G Pab1 protein peptides and proteins unclassified drug cyclic AMP dependent protein kinase catalytic subunit protein aggregate protein subunit Saccharomyces cerevisiae protein TPK2 protein, S cerevisiae Tpk3 protein, S cerevisiae Article cell viability cellular distribution controlled study CYC1 gene ENO2 gene enzyme activity enzyme localization gene deletion gene expression heat stress heat tolerance HSP30 gene HSP42 gene in vitro study nonhuman protein aggregation protein expression protein RNA binding RNA translation Saccharomyces cerevisiae TPK2 gene TPK3 gene translation initiation translation regulation cell fractionation cell granule enzymology heat shock response metabolism physiological stress protein synthesis Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Cytoplasmic Granules Heat-Shock Response Protein Aggregates Protein Biosynthesis Protein Subunits Saccharomyces cerevisiae Proteins Stress, Physiological Subcellular Fractions
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v12_n10_p_Barraza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_19326203_v12_n10_p_Barraza
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spelling	todo:paper_19326203_v12_n10_p_Barraza2023-10-03T16:34:45Z The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae Barraza, C.E. Solari, C.A. Marcovich, I. Kershaw, C. Galello, F. Rossi, S. Ashe, M.P. Portela, P. cyclic AMP dependent protein kinase heat shock protein 30 initiation factor 4E initiation factor 4G Pab1 protein peptides and proteins unclassified drug cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase catalytic subunit protein aggregate protein subunit Saccharomyces cerevisiae protein TPK2 protein, S cerevisiae Tpk3 protein, S cerevisiae Article cell viability cellular distribution controlled study CYC1 gene ENO2 gene enzyme activity enzyme localization gene deletion gene expression heat stress heat tolerance HSP30 gene HSP42 gene in vitro study nonhuman protein aggregation protein expression protein RNA binding RNA translation Saccharomyces cerevisiae TPK2 gene TPK3 gene translation initiation translation regulation cell fractionation cell granule enzymology heat shock response metabolism physiological stress protein subunit protein synthesis Saccharomyces cerevisiae Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Cytoplasmic Granules Heat-Shock Response Protein Aggregates Protein Biosynthesis Protein Subunits Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Subcellular Fractions Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex. Deletion of TPK2 or TPK3 impacts upon the translational response to heat stress of several mRNAs including CYC1, HSP42, HSP30 and ENO2. TPK2 deletion leads to a robust translational arrest, an increase in SGs/PBs aggregation and translational hypersensitivity to heat stress, whereas TPK3 deletion represses SGs/PBs formation, translational arrest and response for the analyzed mRNAs. Therefore, this work provides evidence indicating that Tpk2 and Tpk3 have opposing roles in translational adaptation during heat stress, and highlight how the same signaling pathway can be regulated to generate strikingly distinct physiological outputs. © 2017 Barraza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v12_n10_p_Barraza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic AMP dependent protein kinase heat shock protein 30 initiation factor 4E initiation factor 4G Pab1 protein peptides and proteins unclassified drug cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase catalytic subunit protein aggregate protein subunit Saccharomyces cerevisiae protein TPK2 protein, S cerevisiae Tpk3 protein, S cerevisiae Article cell viability cellular distribution controlled study CYC1 gene ENO2 gene enzyme activity enzyme localization gene deletion gene expression heat stress heat tolerance HSP30 gene HSP42 gene in vitro study nonhuman protein aggregation protein expression protein RNA binding RNA translation Saccharomyces cerevisiae TPK2 gene TPK3 gene translation initiation translation regulation cell fractionation cell granule enzymology heat shock response metabolism physiological stress protein subunit protein synthesis Saccharomyces cerevisiae Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Cytoplasmic Granules Heat-Shock Response Protein Aggregates Protein Biosynthesis Protein Subunits Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Subcellular Fractions
spellingShingle	cyclic AMP dependent protein kinase heat shock protein 30 initiation factor 4E initiation factor 4G Pab1 protein peptides and proteins unclassified drug cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase catalytic subunit protein aggregate protein subunit Saccharomyces cerevisiae protein TPK2 protein, S cerevisiae Tpk3 protein, S cerevisiae Article cell viability cellular distribution controlled study CYC1 gene ENO2 gene enzyme activity enzyme localization gene deletion gene expression heat stress heat tolerance HSP30 gene HSP42 gene in vitro study nonhuman protein aggregation protein expression protein RNA binding RNA translation Saccharomyces cerevisiae TPK2 gene TPK3 gene translation initiation translation regulation cell fractionation cell granule enzymology heat shock response metabolism physiological stress protein subunit protein synthesis Saccharomyces cerevisiae Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Cytoplasmic Granules Heat-Shock Response Protein Aggregates Protein Biosynthesis Protein Subunits Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Subcellular Fractions Barraza, C.E. Solari, C.A. Marcovich, I. Kershaw, C. Galello, F. Rossi, S. Ashe, M.P. Portela, P. The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
topic_facet	cyclic AMP dependent protein kinase heat shock protein 30 initiation factor 4E initiation factor 4G Pab1 protein peptides and proteins unclassified drug cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase catalytic subunit protein aggregate protein subunit Saccharomyces cerevisiae protein TPK2 protein, S cerevisiae Tpk3 protein, S cerevisiae Article cell viability cellular distribution controlled study CYC1 gene ENO2 gene enzyme activity enzyme localization gene deletion gene expression heat stress heat tolerance HSP30 gene HSP42 gene in vitro study nonhuman protein aggregation protein expression protein RNA binding RNA translation Saccharomyces cerevisiae TPK2 gene TPK3 gene translation initiation translation regulation cell fractionation cell granule enzymology heat shock response metabolism physiological stress protein subunit protein synthesis Saccharomyces cerevisiae Cyclic AMP-Dependent Protein Kinase Catalytic Subunits Cyclic AMP-Dependent Protein Kinases Cytoplasmic Granules Heat-Shock Response Protein Aggregates Protein Biosynthesis Protein Subunits Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Subcellular Fractions
description	Cellular responses to stress stem from a variety of different mechanisms, including translation arrest and relocation of the translationally repressed mRNAs to ribonucleoprotein particles like stress granules (SGs) and processing bodies (PBs). Here, we examine the role of PKA in the S. cerevisiae heat shock response. Under mild heat stress Tpk3 aggregates and promotes aggregation of eIF4G, Pab1 and eIF4E, whereas severe heat stress leads to the formation of PBs and SGs that contain both Tpk2 and Tpk3 and a larger 48S translation initiation complex. Deletion of TPK2 or TPK3 impacts upon the translational response to heat stress of several mRNAs including CYC1, HSP42, HSP30 and ENO2. TPK2 deletion leads to a robust translational arrest, an increase in SGs/PBs aggregation and translational hypersensitivity to heat stress, whereas TPK3 deletion represses SGs/PBs formation, translational arrest and response for the analyzed mRNAs. Therefore, this work provides evidence indicating that Tpk2 and Tpk3 have opposing roles in translational adaptation during heat stress, and highlight how the same signaling pathway can be regulated to generate strikingly distinct physiological outputs. © 2017 Barraza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	JOUR
author	Barraza, C.E. Solari, C.A. Marcovich, I. Kershaw, C. Galello, F. Rossi, S. Ashe, M.P. Portela, P.
author_facet	Barraza, C.E. Solari, C.A. Marcovich, I. Kershaw, C. Galello, F. Rossi, S. Ashe, M.P. Portela, P.
author_sort	Barraza, C.E.
title	The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
title_short	The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
title_full	The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
title_fullStr	The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
title_full_unstemmed	The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae
title_sort	role of pka in the translational response to heat stress in saccharomyces cerevisiae
url	http://hdl.handle.net/20.500.12110/paper_19326203_v12_n10_p_Barraza
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The role of PKA in the translational response to heat stress in Saccharomyces cerevisiae

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