Dynamics of T-junction solution switching aimed at patch clamp experiments
Solutions exchange systems are responsible for the timing of drug application on patch clamp experiments. There are two basic strategies for generating a solution exchange. When slow exchanges are bearable, it is easier to perform the exchange inside the tubing system upstream of the exit port. On t...
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todo:paper_19326203_v10_n7_p_Auzmendi2023-10-03T16:34:38Z Dynamics of T-junction solution switching aimed at patch clamp experiments Auzmendi, J.A. Smoler, M. Moffatt, L. sodium chloride solution and solubility Article controlled study equipment design experimental study fluid transport laboratory device mathematical model molecular dynamics molecular interaction patch clamp technique pipette T-junction solution exhanger tube devices pressure procedures solution and solubility time factor Patch-Clamp Techniques Pressure Solutions Time Factors Solutions exchange systems are responsible for the timing of drug application on patch clamp experiments. There are two basic strategies for generating a solution exchange. When slow exchanges are bearable, it is easier to perform the exchange inside the tubing system upstream of the exit port. On the other hand, fast, reproducible, exchanges are usually performed downstream of the exit port. As both strategies are combinable, increasing the performance of upstream exchanges is desirable. We designed a simple method for manufacturing T-junctions (300 μm I.D.) and we measured the time profile of exchange of two saline solutions using a patch pipette with an open tip. Three factors were found to determine the timing of the solution switching: pressure, travelled distance and off-center distance. A linear relationship between the time delay and the travelled distance was found for each tested pressure, showing its dependence to the fluid velocity, which increased with pressure. The exchange time was found to increase quadratically with the delay, although a sizeable variability remains unexplained by this relationship. The delay and exchange times increased as the recording pipette moved away from the center of the stream. Those increases became dramatic as the pipette was moved close to the stream borders. Mass transport along the travelled distance between the slow fluid at the border and the fast fluid at the center seems to contribute to the time course of the solution exchange. This effect would be present in all tubing based devices. Present results might be of fundamental importance for the adequate design of serial compound exchangers which would be instrumental in the discovery of drugs that modulate the action of the physiological agonists of ion channels with the purpose of fine tuning their physiology. Copyright: © 2015 Auzmendi et al. Fil:Auzmendi, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moffatt, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v10_n7_p_Auzmendi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
sodium chloride solution and solubility Article controlled study equipment design experimental study fluid transport laboratory device mathematical model molecular dynamics molecular interaction patch clamp technique pipette T-junction solution exhanger tube devices pressure procedures solution and solubility time factor Patch-Clamp Techniques Pressure Solutions Time Factors |
| spellingShingle |
sodium chloride solution and solubility Article controlled study equipment design experimental study fluid transport laboratory device mathematical model molecular dynamics molecular interaction patch clamp technique pipette T-junction solution exhanger tube devices pressure procedures solution and solubility time factor Patch-Clamp Techniques Pressure Solutions Time Factors Auzmendi, J.A. Smoler, M. Moffatt, L. Dynamics of T-junction solution switching aimed at patch clamp experiments |
| topic_facet |
sodium chloride solution and solubility Article controlled study equipment design experimental study fluid transport laboratory device mathematical model molecular dynamics molecular interaction patch clamp technique pipette T-junction solution exhanger tube devices pressure procedures solution and solubility time factor Patch-Clamp Techniques Pressure Solutions Time Factors |
| description |
Solutions exchange systems are responsible for the timing of drug application on patch clamp experiments. There are two basic strategies for generating a solution exchange. When slow exchanges are bearable, it is easier to perform the exchange inside the tubing system upstream of the exit port. On the other hand, fast, reproducible, exchanges are usually performed downstream of the exit port. As both strategies are combinable, increasing the performance of upstream exchanges is desirable. We designed a simple method for manufacturing T-junctions (300 μm I.D.) and we measured the time profile of exchange of two saline solutions using a patch pipette with an open tip. Three factors were found to determine the timing of the solution switching: pressure, travelled distance and off-center distance. A linear relationship between the time delay and the travelled distance was found for each tested pressure, showing its dependence to the fluid velocity, which increased with pressure. The exchange time was found to increase quadratically with the delay, although a sizeable variability remains unexplained by this relationship. The delay and exchange times increased as the recording pipette moved away from the center of the stream. Those increases became dramatic as the pipette was moved close to the stream borders. Mass transport along the travelled distance between the slow fluid at the border and the fast fluid at the center seems to contribute to the time course of the solution exchange. This effect would be present in all tubing based devices. Present results might be of fundamental importance for the adequate design of serial compound exchangers which would be instrumental in the discovery of drugs that modulate the action of the physiological agonists of ion channels with the purpose of fine tuning their physiology. Copyright: © 2015 Auzmendi et al. |
| format |
JOUR |
| author |
Auzmendi, J.A. Smoler, M. Moffatt, L. |
| author_facet |
Auzmendi, J.A. Smoler, M. Moffatt, L. |
| author_sort |
Auzmendi, J.A. |
| title |
Dynamics of T-junction solution switching aimed at patch clamp experiments |
| title_short |
Dynamics of T-junction solution switching aimed at patch clamp experiments |
| title_full |
Dynamics of T-junction solution switching aimed at patch clamp experiments |
| title_fullStr |
Dynamics of T-junction solution switching aimed at patch clamp experiments |
| title_full_unstemmed |
Dynamics of T-junction solution switching aimed at patch clamp experiments |
| title_sort |
dynamics of t-junction solution switching aimed at patch clamp experiments |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v10_n7_p_Auzmendi |
| work_keys_str_mv |
AT auzmendija dynamicsoftjunctionsolutionswitchingaimedatpatchclampexperiments AT smolerm dynamicsoftjunctionsolutionswitchingaimedatpatchclampexperiments AT moffattl dynamicsoftjunctionsolutionswitchingaimedatpatchclampexperiments |
| _version_ |
1782025137311186944 |