Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors

Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional r...

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Autores principales:	Solari, C., Echegaray, C.V., Cosentino, M.S., Petrone, M.V., Waisman, A., Luzzani, C., Francia, M., Villodre, E., Lenz, G., Miriuka, S., Barañao, L., Guberman, A.
Formato:	JOUR
Materias:	manganese superoxide dismutase messenger RNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 homeodomain protein Nanog protein, mouse Pou5f1 protein, mouse small interfering RNA superoxide dismutase animal cell Article binding site cell differentiation cell renewal cellular parameters computer model controlled study down regulation embryo embryonic stem cell gene expression profiling gene expression regulation gene identification gene repression gene sequence molecular cloning molecular dynamics mouse Nanog gene nonhuman Oct4 gene oxidative stress pluripotency pluripotent stem cell promoter region Sod2 gene transactivation transcription initiation site transcription regulation animal cell line cytology genetics induced pluripotent stem cell metabolism physiology Animals Cell Differentiation Cell Line Embryonic Stem Cells Gene Expression Regulation Gene Expression Regulation, Enzymologic Homeodomain Proteins Induced Pluripotent Stem Cells Mice Nanog Homeobox Protein Octamer Transcription Factor-3 Promoter Regions, Genetic RNA, Small Interfering Superoxide Dismutase
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Solari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_19326203_v10_n12_p_Solari
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spelling	todo:paper_19326203_v10_n12_p_Solari2023-10-03T16:34:33Z Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors Solari, C. Echegaray, C.V. Cosentino, M.S. Petrone, M.V. Waisman, A. Luzzani, C. Francia, M. Villodre, E. Lenz, G. Miriuka, S. Barañao, L. Guberman, A. manganese superoxide dismutase messenger RNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 homeodomain protein manganese superoxide dismutase Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse small interfering RNA superoxide dismutase transcription factor NANOG animal cell Article binding site cell differentiation cell renewal cellular parameters computer model controlled study down regulation embryo embryonic stem cell gene expression profiling gene expression regulation gene identification gene repression gene sequence molecular cloning molecular dynamics mouse Nanog gene nonhuman Oct4 gene oxidative stress pluripotency pluripotent stem cell promoter region Sod2 gene transactivation transcription initiation site transcription regulation animal cell line cytology genetics induced pluripotent stem cell metabolism physiology Animals Cell Differentiation Cell Line Embryonic Stem Cells Gene Expression Regulation Gene Expression Regulation, Enzymologic Homeodomain Proteins Induced Pluripotent Stem Cells Mice Nanog Homeobox Protein Octamer Transcription Factor-3 Promoter Regions, Genetic RNA, Small Interfering Superoxide Dismutase Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional regulation of the involved genes. The reported modulation of some of these genes led us to hypothesize that some of them could be regulated by the transcription factors critical for self-renewal and pluripotency in embryonic stem cells (ESCs) and in induced pluripotent stem cells (iPSCs). In this work, we studied the expression profile of multiple genes involved in antioxidant defense systems in both ESCs and iPSCs. We found that Manganese superoxide dismutase gene (Mn-Sod/Sod2) was repressed during diverse differentiation protocols showing an expression pattern similar to Nanog gene. Moreover, Sod2 promoter activity was induced by Oct4 and Nanog when we performed a transactivation assay using two different reporter constructions. Finally, we studied Sod2 gene regulation by modulating the expression of Oct4 and Nanog in ESCs by shRNAs and found that downregulation of any of them reduced Sod2 expression. Our results indicate that pluripotency transcription factors positively modulate Sod2 gene transcription. © 2015 Solari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Cosentino, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Solari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	manganese superoxide dismutase messenger RNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 homeodomain protein manganese superoxide dismutase Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse small interfering RNA superoxide dismutase transcription factor NANOG animal cell Article binding site cell differentiation cell renewal cellular parameters computer model controlled study down regulation embryo embryonic stem cell gene expression profiling gene expression regulation gene identification gene repression gene sequence molecular cloning molecular dynamics mouse Nanog gene nonhuman Oct4 gene oxidative stress pluripotency pluripotent stem cell promoter region Sod2 gene transactivation transcription initiation site transcription regulation animal cell line cytology genetics induced pluripotent stem cell metabolism physiology Animals Cell Differentiation Cell Line Embryonic Stem Cells Gene Expression Regulation Gene Expression Regulation, Enzymologic Homeodomain Proteins Induced Pluripotent Stem Cells Mice Nanog Homeobox Protein Octamer Transcription Factor-3 Promoter Regions, Genetic RNA, Small Interfering Superoxide Dismutase
spellingShingle	manganese superoxide dismutase messenger RNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 homeodomain protein manganese superoxide dismutase Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse small interfering RNA superoxide dismutase transcription factor NANOG animal cell Article binding site cell differentiation cell renewal cellular parameters computer model controlled study down regulation embryo embryonic stem cell gene expression profiling gene expression regulation gene identification gene repression gene sequence molecular cloning molecular dynamics mouse Nanog gene nonhuman Oct4 gene oxidative stress pluripotency pluripotent stem cell promoter region Sod2 gene transactivation transcription initiation site transcription regulation animal cell line cytology genetics induced pluripotent stem cell metabolism physiology Animals Cell Differentiation Cell Line Embryonic Stem Cells Gene Expression Regulation Gene Expression Regulation, Enzymologic Homeodomain Proteins Induced Pluripotent Stem Cells Mice Nanog Homeobox Protein Octamer Transcription Factor-3 Promoter Regions, Genetic RNA, Small Interfering Superoxide Dismutase Solari, C. Echegaray, C.V. Cosentino, M.S. Petrone, M.V. Waisman, A. Luzzani, C. Francia, M. Villodre, E. Lenz, G. Miriuka, S. Barañao, L. Guberman, A. Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
topic_facet	manganese superoxide dismutase messenger RNA octamer transcription factor 4 short hairpin RNA transcription factor NANOG transcription factor Sox2 homeodomain protein manganese superoxide dismutase Nanog protein, mouse octamer transcription factor 4 Pou5f1 protein, mouse small interfering RNA superoxide dismutase transcription factor NANOG animal cell Article binding site cell differentiation cell renewal cellular parameters computer model controlled study down regulation embryo embryonic stem cell gene expression profiling gene expression regulation gene identification gene repression gene sequence molecular cloning molecular dynamics mouse Nanog gene nonhuman Oct4 gene oxidative stress pluripotency pluripotent stem cell promoter region Sod2 gene transactivation transcription initiation site transcription regulation animal cell line cytology genetics induced pluripotent stem cell metabolism physiology Animals Cell Differentiation Cell Line Embryonic Stem Cells Gene Expression Regulation Gene Expression Regulation, Enzymologic Homeodomain Proteins Induced Pluripotent Stem Cells Mice Nanog Homeobox Protein Octamer Transcription Factor-3 Promoter Regions, Genetic RNA, Small Interfering Superoxide Dismutase
description	Pluripotent stem cells possess complex systems that protect them from oxidative stress and ensure genomic stability, vital for their role in development. Even though it has been reported that antioxidant activity diminishes along stem cell differentiation, little is known about the transcriptional regulation of the involved genes. The reported modulation of some of these genes led us to hypothesize that some of them could be regulated by the transcription factors critical for self-renewal and pluripotency in embryonic stem cells (ESCs) and in induced pluripotent stem cells (iPSCs). In this work, we studied the expression profile of multiple genes involved in antioxidant defense systems in both ESCs and iPSCs. We found that Manganese superoxide dismutase gene (Mn-Sod/Sod2) was repressed during diverse differentiation protocols showing an expression pattern similar to Nanog gene. Moreover, Sod2 promoter activity was induced by Oct4 and Nanog when we performed a transactivation assay using two different reporter constructions. Finally, we studied Sod2 gene regulation by modulating the expression of Oct4 and Nanog in ESCs by shRNAs and found that downregulation of any of them reduced Sod2 expression. Our results indicate that pluripotency transcription factors positively modulate Sod2 gene transcription. © 2015 Solari et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	JOUR
author	Solari, C. Echegaray, C.V. Cosentino, M.S. Petrone, M.V. Waisman, A. Luzzani, C. Francia, M. Villodre, E. Lenz, G. Miriuka, S. Barañao, L. Guberman, A.
author_facet	Solari, C. Echegaray, C.V. Cosentino, M.S. Petrone, M.V. Waisman, A. Luzzani, C. Francia, M. Villodre, E. Lenz, G. Miriuka, S. Barañao, L. Guberman, A.
author_sort	Solari, C.
title	Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
title_short	Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
title_full	Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
title_fullStr	Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
title_full_unstemmed	Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors
title_sort	manganese superoxide dismutase gene expression is induced by nanog and oct4, essential pluripotent stem cells' transcription factors
url	http://hdl.handle.net/20.500.12110/paper_19326203_v10_n12_p_Solari
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Manganese superoxide dismutase gene expression is induced by Nanog and Oct4, essential pluripotent stem cells' transcription factors

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