Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection
The endocytic uptake and intracellular trafficking for penetration of DENV-3 strain H-87 into Vero cells was analyzed by using several biochemical inhibitors and dominant negative mutants of cellular proteins. The results presented show that the infective entry of DENV-3 into Vero cells occurs throu...
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todo:paper_19326203_v10_n10_p_Piccini2023-10-03T16:34:32Z Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection Piccini, L.E. Castilla, V. Damonte, E.B. ammonium chloride clathrin dynamin Rab7 protein clathrin animal cell Article cell transport controlled study cytosol dengue Dengue virus 3 endocytosis endosome human human cell mammal cell nonhuman pH virus cell interaction virus entry virus nucleocapsid Aedes animal cell culture Chlorocebus aethiops dengue Dengue virus endocytosis Hep-G2 cell line metabolism microbial sensitivity test pathology physiology prognosis U-937 cell line Vero cell line virology Aedes Animals Cells, Cultured Cercopithecus aethiops Clathrin Dengue Dengue Virus Endocytosis Hep G2 Cells Humans Microbial Sensitivity Tests Prognosis U937 Cells Vero Cells Virus Internalization The endocytic uptake and intracellular trafficking for penetration of DENV-3 strain H-87 into Vero cells was analyzed by using several biochemical inhibitors and dominant negative mutants of cellular proteins. The results presented show that the infective entry of DENV-3 into Vero cells occurs through a non-classical endocytosis pathway dependent on low pH and dynamin, but non-mediated by clathrin. After uptake, DENV-3 transits through early endosomes to reach Rab 7-regulated late endosomes, and according with the half-time for ammonium chloride resistance viral nucleocapsid is released into the cytosol approximately at 12 min post-infection. Furthermore, the influence of the clathrin pathway in DENV-3 infective entry in other mammalian cell lines of human origin, such as A549, HepG2 and U937 cells, was evaluated demonstrating that variable entry pathways are employed depending on the host cell. Results show for the first time the simultaneous coexistence of infective and non -infective routes for DENV entry into the host cell, depending on the usage of clathrin-mediated endocytosis. © 2015 Piccini et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19326203_v10_n10_p_Piccini |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ammonium chloride clathrin dynamin Rab7 protein clathrin animal cell Article cell transport controlled study cytosol dengue Dengue virus 3 endocytosis endosome human human cell mammal cell nonhuman pH virus cell interaction virus entry virus nucleocapsid Aedes animal cell culture Chlorocebus aethiops dengue Dengue virus endocytosis Hep-G2 cell line metabolism microbial sensitivity test pathology physiology prognosis U-937 cell line Vero cell line virology Aedes Animals Cells, Cultured Cercopithecus aethiops Clathrin Dengue Dengue Virus Endocytosis Hep G2 Cells Humans Microbial Sensitivity Tests Prognosis U937 Cells Vero Cells Virus Internalization |
| spellingShingle |
ammonium chloride clathrin dynamin Rab7 protein clathrin animal cell Article cell transport controlled study cytosol dengue Dengue virus 3 endocytosis endosome human human cell mammal cell nonhuman pH virus cell interaction virus entry virus nucleocapsid Aedes animal cell culture Chlorocebus aethiops dengue Dengue virus endocytosis Hep-G2 cell line metabolism microbial sensitivity test pathology physiology prognosis U-937 cell line Vero cell line virology Aedes Animals Cells, Cultured Cercopithecus aethiops Clathrin Dengue Dengue Virus Endocytosis Hep G2 Cells Humans Microbial Sensitivity Tests Prognosis U937 Cells Vero Cells Virus Internalization Piccini, L.E. Castilla, V. Damonte, E.B. Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| topic_facet |
ammonium chloride clathrin dynamin Rab7 protein clathrin animal cell Article cell transport controlled study cytosol dengue Dengue virus 3 endocytosis endosome human human cell mammal cell nonhuman pH virus cell interaction virus entry virus nucleocapsid Aedes animal cell culture Chlorocebus aethiops dengue Dengue virus endocytosis Hep-G2 cell line metabolism microbial sensitivity test pathology physiology prognosis U-937 cell line Vero cell line virology Aedes Animals Cells, Cultured Cercopithecus aethiops Clathrin Dengue Dengue Virus Endocytosis Hep G2 Cells Humans Microbial Sensitivity Tests Prognosis U937 Cells Vero Cells Virus Internalization |
| description |
The endocytic uptake and intracellular trafficking for penetration of DENV-3 strain H-87 into Vero cells was analyzed by using several biochemical inhibitors and dominant negative mutants of cellular proteins. The results presented show that the infective entry of DENV-3 into Vero cells occurs through a non-classical endocytosis pathway dependent on low pH and dynamin, but non-mediated by clathrin. After uptake, DENV-3 transits through early endosomes to reach Rab 7-regulated late endosomes, and according with the half-time for ammonium chloride resistance viral nucleocapsid is released into the cytosol approximately at 12 min post-infection. Furthermore, the influence of the clathrin pathway in DENV-3 infective entry in other mammalian cell lines of human origin, such as A549, HepG2 and U937 cells, was evaluated demonstrating that variable entry pathways are employed depending on the host cell. Results show for the first time the simultaneous coexistence of infective and non -infective routes for DENV entry into the host cell, depending on the usage of clathrin-mediated endocytosis. © 2015 Piccini et al This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| format |
JOUR |
| author |
Piccini, L.E. Castilla, V. Damonte, E.B. |
| author_facet |
Piccini, L.E. Castilla, V. Damonte, E.B. |
| author_sort |
Piccini, L.E. |
| title |
Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| title_short |
Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| title_full |
Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| title_fullStr |
Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| title_full_unstemmed |
Dengue-3 virus entry into vero cells: Role of clathrin-mediated endocytosis in the outcome of infection |
| title_sort |
dengue-3 virus entry into vero cells: role of clathrin-mediated endocytosis in the outcome of infection |
| url |
http://hdl.handle.net/20.500.12110/paper_19326203_v10_n10_p_Piccini |
| work_keys_str_mv |
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