Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol

In bufonids, the Bidder's organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express se...

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Detalles Bibliográficos
Autores principales: Scaia, M.F., Regueira, E., Volonteri, M.C., Ceballos, N.R.
Formato: JOUR
Materias:
aromatase
cholesterol
estradiol
animal
article
blood
Bufonidae
female
male
metabolism
ovary follicle
season
testis
Animals
Aromatase
Cholesterol
Estradiol
Female
Male
Ovarian Follicle
Seasons
Testis
Amphibia
Animalia
Anura
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_19325223_v319_n6_p355_Scaia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_19325223_v319_n6_p355_Scaia2023-10-03T16:34:30Z Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol Scaia, M.F. Regueira, E. Volonteri, M.C. Ceballos, N.R. aromatase cholesterol estradiol animal article blood Bufonidae female male metabolism ovary follicle season testis Animals Aromatase Bufonidae Cholesterol Estradiol Female Male Ovarian Follicle Seasons Testis Amphibia Animalia Anura Bufonidae In bufonids, the Bidder's organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre-reproductive season than during the reproductive and post-reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates. J. Exp. Zool. 319A:355-364, 2013. © 2013 Wiley Periodicals, Inc. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19325223_v319_n6_p355_Scaia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic aromatase
cholesterol
estradiol
animal
article
blood
Bufonidae
female
male
metabolism
ovary follicle
season
testis
Animals
Aromatase
Bufonidae
Cholesterol
Estradiol
Female
Male
Ovarian Follicle
Seasons
Testis
Amphibia
Animalia
Anura
Bufonidae
spellingShingle aromatase
cholesterol
estradiol
animal
article
blood
Bufonidae
female
male
metabolism
ovary follicle
season
testis
Animals
Aromatase
Bufonidae
Cholesterol
Estradiol
Female
Male
Ovarian Follicle
Seasons
Testis
Amphibia
Animalia
Anura
Bufonidae
Scaia, M.F.
Regueira, E.
Volonteri, M.C.
Ceballos, N.R.
Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
topic_facet aromatase
cholesterol
estradiol
animal
article
blood
Bufonidae
female
male
metabolism
ovary follicle
season
testis
Animals
Aromatase
Bufonidae
Cholesterol
Estradiol
Female
Male
Ovarian Follicle
Seasons
Testis
Amphibia
Animalia
Anura
Bufonidae
description In bufonids, the Bidder's organ (BO), located in the anterior pole of the testis, is sometimes referred to as a rudimentary ovary because of the presence of previtellogenic follicles. In males of Rhinella arenarum it has been demonstrated that some follicles are vitellogenic and also express several steroidogenic enzymes in follicular cells. The purpose of this study is to describe seasonal variations in plasma estradiol (E2) and in aromatase activity of the BO, and to determine the capacity of the BO to synthesize E2 from cholesterol in males of R. arenarum. E2 was determined by radioimmunoassay and aromatase activity was measured by transformation of radioactive substrates into products. Results indicate that plasma E2 reached the highest concentration in April and the lowest one in animals captured in June, showing a progressive increase to the end of the year. Plasma E2 and total activity of aromatase in the BO were significantly lower during the pre-reproductive season than during the reproductive and post-reproductive seasons. It was also demonstrated that the BO is able to produce E2 from endogenous substrates throughout the year. No correlation was found between plasma E2 and total BO weight, while there was a significant correlation between plasma E2 and total activity of aromatase, and between plasma E2 and E2 produced in vitro. Taken together, these results demonstrate seasonal variations in plasma E2, in bidderian total activity of aromatase and, that the BO of adult males of R. arenarum is able to produce E2 from endogenous substrates. J. Exp. Zool. 319A:355-364, 2013. © 2013 Wiley Periodicals, Inc.
format JOUR
author Scaia, M.F.
Regueira, E.
Volonteri, M.C.
Ceballos, N.R.
author_facet Scaia, M.F.
Regueira, E.
Volonteri, M.C.
Ceballos, N.R.
author_sort Scaia, M.F.
title Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
title_short Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
title_full Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
title_fullStr Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
title_full_unstemmed Estradiol Production by the Bidder's Organ of the Toad Rhinella arenarum (Amphibia, Anura). Seasonal Variations in Plasma Estradiol
title_sort estradiol production by the bidder's organ of the toad rhinella arenarum (amphibia, anura). seasonal variations in plasma estradiol
url http://hdl.handle.net/20.500.12110/paper_19325223_v319_n6_p355_Scaia
work_keys_str_mv AT scaiamf estradiolproductionbythebiddersorganofthetoadrhinellaarenarumamphibiaanuraseasonalvariationsinplasmaestradiol
AT regueirae estradiolproductionbythebiddersorganofthetoadrhinellaarenarumamphibiaanuraseasonalvariationsinplasmaestradiol
AT volonterimc estradiolproductionbythebiddersorganofthetoadrhinellaarenarumamphibiaanuraseasonalvariationsinplasmaestradiol
AT ceballosnr estradiolproductionbythebiddersorganofthetoadrhinellaarenarumamphibiaanuraseasonalvariationsinplasmaestradiol
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