The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes
The Bidder's organ (BO) of male true toads of Bufonidae family is located in the anterior pole of the testis and it has been compared to a rudimentary ovary because of the presence of previtellogenic follicles. In some species, BO remains in both sexes, while in others only adult males preserve...
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todo:paper_19325223_v315A_n8_p439_Scaia2023-10-03T16:34:29Z The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes Scaia, M.F. Regueira, E. Sassone, A.G. Volonteri, M.C. Ceballos, N.R. 3(or 17)beta hydroxysteroid dehydrogenase aromatase steroid 17alpha monooxygenase animal article enzymology female histology male ovary follicle testis toad vitellogenesis 3-Hydroxysteroid Dehydrogenases Animals Aromatase Bufonidae Female Male Ovarian Follicle Steroid 17-alpha-Hydroxylase Testis Vitellogenesis Amphibia Anura Bufonidae The Bidder's organ (BO) of male true toads of Bufonidae family is located in the anterior pole of the testis and it has been compared to a rudimentary ovary because of the presence of previtellogenic follicles. In some species, BO remains in both sexes, while in others only adult males preserve the structure. Several studies suggest that the development of BO is inhibited by the differentiation of the corresponding gonad. The purpose of this study is to describe morphological and histological variability of the BO of Rhinella arenarum and also analyze its steroidogenic capacity. Observations indicate that although most bidderian follicles are in pre vitellogenesis, there are others in early or late vitellogenesis. Moreover, we found that BOs weight was significantly lower in males during the pre-reproductive period and that there is no significant correlation between the weights of BO and the adjacent testis. We also analyzed the presence of steroidogenic enzymes using immunohistochemistry. Results indicate that all the follicles were immunoreactive with the antibody against aromatase, while only few of them were positive for the cytochrome P450 side-chain cleavage. Furthermore, activities of 3β-hydroxysteroid dehydrogenase/isomerase, cytochrome P450 17-hydroxylase, C17,20-lyase and aromatase were detected by the transformation of radioactive substrates into products. Taken together, these results confirm the steroidogenic capacity of the BO in adult males of R. arenarum. © 2011 Wiley-Liss, Inc., A Wiley Company. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sassone, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_19325223_v315A_n8_p439_Scaia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3(or 17)beta hydroxysteroid dehydrogenase aromatase steroid 17alpha monooxygenase animal article enzymology female histology male ovary follicle testis toad vitellogenesis 3-Hydroxysteroid Dehydrogenases Animals Aromatase Bufonidae Female Male Ovarian Follicle Steroid 17-alpha-Hydroxylase Testis Vitellogenesis Amphibia Anura Bufonidae |
| spellingShingle |
3(or 17)beta hydroxysteroid dehydrogenase aromatase steroid 17alpha monooxygenase animal article enzymology female histology male ovary follicle testis toad vitellogenesis 3-Hydroxysteroid Dehydrogenases Animals Aromatase Bufonidae Female Male Ovarian Follicle Steroid 17-alpha-Hydroxylase Testis Vitellogenesis Amphibia Anura Bufonidae Scaia, M.F. Regueira, E. Sassone, A.G. Volonteri, M.C. Ceballos, N.R. The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| topic_facet |
3(or 17)beta hydroxysteroid dehydrogenase aromatase steroid 17alpha monooxygenase animal article enzymology female histology male ovary follicle testis toad vitellogenesis 3-Hydroxysteroid Dehydrogenases Animals Aromatase Bufonidae Female Male Ovarian Follicle Steroid 17-alpha-Hydroxylase Testis Vitellogenesis Amphibia Anura Bufonidae |
| description |
The Bidder's organ (BO) of male true toads of Bufonidae family is located in the anterior pole of the testis and it has been compared to a rudimentary ovary because of the presence of previtellogenic follicles. In some species, BO remains in both sexes, while in others only adult males preserve the structure. Several studies suggest that the development of BO is inhibited by the differentiation of the corresponding gonad. The purpose of this study is to describe morphological and histological variability of the BO of Rhinella arenarum and also analyze its steroidogenic capacity. Observations indicate that although most bidderian follicles are in pre vitellogenesis, there are others in early or late vitellogenesis. Moreover, we found that BOs weight was significantly lower in males during the pre-reproductive period and that there is no significant correlation between the weights of BO and the adjacent testis. We also analyzed the presence of steroidogenic enzymes using immunohistochemistry. Results indicate that all the follicles were immunoreactive with the antibody against aromatase, while only few of them were positive for the cytochrome P450 side-chain cleavage. Furthermore, activities of 3β-hydroxysteroid dehydrogenase/isomerase, cytochrome P450 17-hydroxylase, C17,20-lyase and aromatase were detected by the transformation of radioactive substrates into products. Taken together, these results confirm the steroidogenic capacity of the BO in adult males of R. arenarum. © 2011 Wiley-Liss, Inc., A Wiley Company. |
| format |
JOUR |
| author |
Scaia, M.F. Regueira, E. Sassone, A.G. Volonteri, M.C. Ceballos, N.R. |
| author_facet |
Scaia, M.F. Regueira, E. Sassone, A.G. Volonteri, M.C. Ceballos, N.R. |
| author_sort |
Scaia, M.F. |
| title |
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| title_short |
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| title_full |
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| title_fullStr |
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| title_full_unstemmed |
The Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura). Presence of steroidogenic enzymes |
| title_sort |
bidder's organ of the toad rhinella arenarum (amphibia, anura). presence of steroidogenic enzymes |
| url |
http://hdl.handle.net/20.500.12110/paper_19325223_v315A_n8_p439_Scaia |
| work_keys_str_mv |
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1807318980023549952 |