PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae
Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated th...
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todo:paper_18749399_v1849_n11_p1329_Baccarini2023-10-03T16:34:10Z PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae Baccarini, L. Martínez-Montañés, F. Rossi, S. Proft, M. Portela, P. Chromatin Osmotic stress PKA Saccharomyces cerevisiae karyopherin beta chromatin cyclic AMP dependent protein kinase Saccharomyces cerevisiae protein ald6 gene Article cell survival chromatin chromatin assembly and disassembly fungal gene gene deletion gene expression regulation gene targeting genetic analysis hsp42 gene nonhuman osmotic stress pKa priority journal promoter region rps29b gene Saccharomyces cerevisiae sed1 gene upregulation biosynthesis chromatin enzymology genetics metabolism physiological stress physiology Saccharomyces cerevisiae Chromatin Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target genes in a carbon source and oxidative stress dependent manner. Here we studied five genes, ALD6, SED1, HSP42, RPS29B, and RPL1B whose expression is regulated by saline stress. We found that PKA catalytic and regulatory subunits are associated with both coding regions and promoters of the analyzed genes in a stress dependent manner. Tpk1 and Tpk2 recruitment was completely abolished in catalytic inactive mutants. BCY1 deletion changed the binding kinetic to chromatin of each Tpk isoform and this strain displayed a deregulated gene expression in response to osmotic stress. In addition, yeast mutants with high PKA activity exhibit sustained association to target genes of chromatin-remodeling complexes such as Snf2-catalytic subunit of the SWI/SNF complex and Arp8-component of INO80 complex, leading to upregulation of gene expression during osmotic stress. Tpk1 accumulation in the nucleus was stimulated upon osmotic stress, while the nuclear localization of Tpk2 and Bcy1 showed no change. We found that each PKA subunit is transported into the nucleus by a different β-karyopherin pathway. Moreover, β-karyopherin mutant strains abolished the chromatin association of Tpk1 or Tpk2, suggesting that nuclear localization of PKA catalytic subunits is required for its association to target genes and properly gene expression. © 2015 Elsevier B.V. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Portela, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_18749399_v1849_n11_p1329_Baccarini |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Chromatin Osmotic stress PKA Saccharomyces cerevisiae karyopherin beta chromatin cyclic AMP dependent protein kinase Saccharomyces cerevisiae protein ald6 gene Article cell survival chromatin chromatin assembly and disassembly fungal gene gene deletion gene expression regulation gene targeting genetic analysis hsp42 gene nonhuman osmotic stress pKa priority journal promoter region rps29b gene Saccharomyces cerevisiae sed1 gene upregulation biosynthesis chromatin enzymology genetics metabolism physiological stress physiology Saccharomyces cerevisiae Chromatin Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological |
spellingShingle |
Chromatin Osmotic stress PKA Saccharomyces cerevisiae karyopherin beta chromatin cyclic AMP dependent protein kinase Saccharomyces cerevisiae protein ald6 gene Article cell survival chromatin chromatin assembly and disassembly fungal gene gene deletion gene expression regulation gene targeting genetic analysis hsp42 gene nonhuman osmotic stress pKa priority journal promoter region rps29b gene Saccharomyces cerevisiae sed1 gene upregulation biosynthesis chromatin enzymology genetics metabolism physiological stress physiology Saccharomyces cerevisiae Chromatin Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological Baccarini, L. Martínez-Montañés, F. Rossi, S. Proft, M. Portela, P. PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
topic_facet |
Chromatin Osmotic stress PKA Saccharomyces cerevisiae karyopherin beta chromatin cyclic AMP dependent protein kinase Saccharomyces cerevisiae protein ald6 gene Article cell survival chromatin chromatin assembly and disassembly fungal gene gene deletion gene expression regulation gene targeting genetic analysis hsp42 gene nonhuman osmotic stress pKa priority journal promoter region rps29b gene Saccharomyces cerevisiae sed1 gene upregulation biosynthesis chromatin enzymology genetics metabolism physiological stress physiology Saccharomyces cerevisiae Chromatin Cyclic AMP-Dependent Protein Kinases Gene Expression Regulation, Fungal Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Stress, Physiological |
description |
Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target genes in a carbon source and oxidative stress dependent manner. Here we studied five genes, ALD6, SED1, HSP42, RPS29B, and RPL1B whose expression is regulated by saline stress. We found that PKA catalytic and regulatory subunits are associated with both coding regions and promoters of the analyzed genes in a stress dependent manner. Tpk1 and Tpk2 recruitment was completely abolished in catalytic inactive mutants. BCY1 deletion changed the binding kinetic to chromatin of each Tpk isoform and this strain displayed a deregulated gene expression in response to osmotic stress. In addition, yeast mutants with high PKA activity exhibit sustained association to target genes of chromatin-remodeling complexes such as Snf2-catalytic subunit of the SWI/SNF complex and Arp8-component of INO80 complex, leading to upregulation of gene expression during osmotic stress. Tpk1 accumulation in the nucleus was stimulated upon osmotic stress, while the nuclear localization of Tpk2 and Bcy1 showed no change. We found that each PKA subunit is transported into the nucleus by a different β-karyopherin pathway. Moreover, β-karyopherin mutant strains abolished the chromatin association of Tpk1 or Tpk2, suggesting that nuclear localization of PKA catalytic subunits is required for its association to target genes and properly gene expression. © 2015 Elsevier B.V. |
format |
JOUR |
author |
Baccarini, L. Martínez-Montañés, F. Rossi, S. Proft, M. Portela, P. |
author_facet |
Baccarini, L. Martínez-Montañés, F. Rossi, S. Proft, M. Portela, P. |
author_sort |
Baccarini, L. |
title |
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
title_short |
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
title_full |
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
title_fullStr |
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
title_full_unstemmed |
PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae |
title_sort |
pka-chromatin association at stress responsive target genes from saccharomyces cerevisiae |
url |
http://hdl.handle.net/20.500.12110/paper_18749399_v1849_n11_p1329_Baccarini |
work_keys_str_mv |
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_version_ |
1782027399087521792 |