Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics

Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling in situ living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled...

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Autores principales:	Gholipour, Y., Erra-Balsells, R., Nonami, H.
Formato:	JOUR
Materias:	MALDI Metabolite profiling NanoESI Tulip Cell pressure Cell sap Integrative analysis Kestose Key feature MALDI-mass spectrometry MALDI-TOF mass spectrometry Metabolite content Metabolite profiles Metabolomics Nano-ESI Plant cells Secondary metabolites Spectrometry technique Sucrose concentration Turgor pressure Water status Amino acids Biomolecules Carbohydrates Mass spectrometry Physiology Plant cell culture Plants (botany) Sugar (sucrose) Tissue Metabolites
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1816093X_v20_n4_p294_Gholipour
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1816093X_v20_n4_p294_Gholipour
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spelling	todo:paper_1816093X_v20_n4_p294_Gholipour2023-10-03T16:33:02Z Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics Gholipour, Y. Erra-Balsells, R. Nonami, H. MALDI Metabolite profiling NanoESI Tulip Cell pressure Cell sap Integrative analysis Kestose Key feature MALDI MALDI-mass spectrometry MALDI-TOF mass spectrometry Metabolite content Metabolite profiles Metabolite profiling Metabolomics Nano-ESI Plant cells Secondary metabolites Spectrometry technique Sucrose concentration Tulip Turgor pressure Water status Amino acids Biomolecules Carbohydrates Mass spectrometry Physiology Plant cell culture Plants (botany) Sugar (sucrose) Tissue Metabolites Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling in situ living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1816093X_v20_n4_p294_Gholipour
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	MALDI Metabolite profiling NanoESI Tulip Cell pressure Cell sap Integrative analysis Kestose Key feature MALDI MALDI-mass spectrometry MALDI-TOF mass spectrometry Metabolite content Metabolite profiles Metabolite profiling Metabolomics Nano-ESI Plant cells Secondary metabolites Spectrometry technique Sucrose concentration Tulip Turgor pressure Water status Amino acids Biomolecules Carbohydrates Mass spectrometry Physiology Plant cell culture Plants (botany) Sugar (sucrose) Tissue Metabolites
spellingShingle	MALDI Metabolite profiling NanoESI Tulip Cell pressure Cell sap Integrative analysis Kestose Key feature MALDI MALDI-mass spectrometry MALDI-TOF mass spectrometry Metabolite content Metabolite profiles Metabolite profiling Metabolomics Nano-ESI Plant cells Secondary metabolites Spectrometry technique Sucrose concentration Tulip Turgor pressure Water status Amino acids Biomolecules Carbohydrates Mass spectrometry Physiology Plant cell culture Plants (botany) Sugar (sucrose) Tissue Metabolites Gholipour, Y. Erra-Balsells, R. Nonami, H. Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
topic_facet	MALDI Metabolite profiling NanoESI Tulip Cell pressure Cell sap Integrative analysis Kestose Key feature MALDI MALDI-mass spectrometry MALDI-TOF mass spectrometry Metabolite content Metabolite profiles Metabolite profiling Metabolomics Nano-ESI Plant cells Secondary metabolites Spectrometry technique Sucrose concentration Tulip Turgor pressure Water status Amino acids Biomolecules Carbohydrates Mass spectrometry Physiology Plant cell culture Plants (botany) Sugar (sucrose) Tissue Metabolites
description	Water status and metabolite content are considered as two key features in plant cell physiological phenotype. In order to profiling in situ living plant cell status, turgor pressure of cells located at different locations of tissues was probed with a cell pressure probe and then cell sap was sampled and its metabolite profile was generated with using nanoESI and MALDI mass spectrometry. No purification or separation was included in workflow and picoliter cell sap samples were injected directly into a nanoESI-Orbitrap mass spectrometer and/or deposited on selected matrices from organic compounds and nanoparticles for MALDI-TOF mass spectrometry analysis. Both shotgun mass spectrometry techniques could be used for detecting and quantifying metabolites in single-cell samples. Different metabolites from neutral carbohydrates to amino acids and secondary metabolites could be detected. Quantity of two major metabolites, sucrose and kestose, was also measured in several cells and sucrose concentration was co-plotted with turgor data.
format	JOUR
author	Gholipour, Y. Erra-Balsells, R. Nonami, H.
author_facet	Gholipour, Y. Erra-Balsells, R. Nonami, H.
author_sort	Gholipour, Y.
title	Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
title_short	Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
title_full	Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
title_fullStr	Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
title_full_unstemmed	Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
title_sort	integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics
url	http://hdl.handle.net/20.500.12110/paper_1816093X_v20_n4_p294_Gholipour
work_keys_str_mv	AT gholipoury integrativeanalysisofphysiologicalphenotypeofplantcellsbyturgormeasurementandmetabolomics AT errabalsellsr integrativeanalysisofphysiologicalphenotypeofplantcellsbyturgormeasurementandmetabolomics AT nonamih integrativeanalysisofphysiologicalphenotypeofplantcellsbyturgormeasurementandmetabolomics
_version_	1807319190983409664

Integrative analysis of physiological phenotype of plant cells byturgor measurement and metabolomics

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