Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes
Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolyti...
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todo:paper_17912997_v4_n5_p955_Pagano2023-10-03T16:32:53Z Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes Pagano, E. Fontana, V. Calvo, J.C. 3T3-L1 cells erbB2 erbB3 erbB4 Erythroblastic leukemia viral oncogene homolog (erbB) 1/epidermal growth factor receptor Heregulin isoform 2-extracellular domain Herstatin Preadipocytes complementary DNA epidermal growth factor receptor epidermal growth factor receptor 2 epidermal growth factor receptor 3 epidermal growth factor receptor 4 messenger RNA animal cell article cell strain 3T3 mouse nonhuman nucleotide sequence proadipocyte protein analysis protein expression 3T3-L1 Cells Adipocytes Alternative Splicing Animals Cell Proliferation Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Mice Rats Receptor, Epidermal Growth Factor Receptor, erbB-2 Receptor, erbB-3 Receptors, Growth Factor RNA, Messenger Rattus Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolytic erbB variants, was evaluated using RT-PCR. Epidermal growth factor receptor (EGFR)/erbB1 expression was confirmed. Wild-type (wt) ErbB2, human erbB2 (HER2)-extracellular domain (ECD) and herstatin mRNA expression were analyzed. Restriction analysis confirmed wt expression. 3T3-L1 cells exhibited HER2-ECD and herstatin mRNA expression, although at extremely low levels (compared to the control cell lines). ErbB3 cDNA was amplified using mouse mammary tumors and rat acines as positive controls. ErbB4 was not positively identified in these cells. In conclusion, this study demonstrates that 3T3-L1 cells express EGFR/erbB1, erbB2 and erbB3, and possibly, certain erbB2 splice or proteolytic variants, which are worth pursuing. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17912997_v4_n5_p955_Pagano |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3T3-L1 cells erbB2 erbB3 erbB4 Erythroblastic leukemia viral oncogene homolog (erbB) 1/epidermal growth factor receptor Heregulin isoform 2-extracellular domain Herstatin Preadipocytes complementary DNA epidermal growth factor receptor epidermal growth factor receptor 2 epidermal growth factor receptor 3 epidermal growth factor receptor 4 messenger RNA animal cell article cell strain 3T3 mouse nonhuman nucleotide sequence proadipocyte protein analysis protein expression 3T3-L1 Cells Adipocytes Alternative Splicing Animals Cell Proliferation Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Mice Rats Receptor, Epidermal Growth Factor Receptor, erbB-2 Receptor, erbB-3 Receptors, Growth Factor RNA, Messenger Rattus |
| spellingShingle |
3T3-L1 cells erbB2 erbB3 erbB4 Erythroblastic leukemia viral oncogene homolog (erbB) 1/epidermal growth factor receptor Heregulin isoform 2-extracellular domain Herstatin Preadipocytes complementary DNA epidermal growth factor receptor epidermal growth factor receptor 2 epidermal growth factor receptor 3 epidermal growth factor receptor 4 messenger RNA animal cell article cell strain 3T3 mouse nonhuman nucleotide sequence proadipocyte protein analysis protein expression 3T3-L1 Cells Adipocytes Alternative Splicing Animals Cell Proliferation Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Mice Rats Receptor, Epidermal Growth Factor Receptor, erbB-2 Receptor, erbB-3 Receptors, Growth Factor RNA, Messenger Rattus Pagano, E. Fontana, V. Calvo, J.C. Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| topic_facet |
3T3-L1 cells erbB2 erbB3 erbB4 Erythroblastic leukemia viral oncogene homolog (erbB) 1/epidermal growth factor receptor Heregulin isoform 2-extracellular domain Herstatin Preadipocytes complementary DNA epidermal growth factor receptor epidermal growth factor receptor 2 epidermal growth factor receptor 3 epidermal growth factor receptor 4 messenger RNA animal cell article cell strain 3T3 mouse nonhuman nucleotide sequence proadipocyte protein analysis protein expression 3T3-L1 Cells Adipocytes Alternative Splicing Animals Cell Proliferation Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Mice Rats Receptor, Epidermal Growth Factor Receptor, erbB-2 Receptor, erbB-3 Receptors, Growth Factor RNA, Messenger Rattus |
| description |
Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolytic erbB variants, was evaluated using RT-PCR. Epidermal growth factor receptor (EGFR)/erbB1 expression was confirmed. Wild-type (wt) ErbB2, human erbB2 (HER2)-extracellular domain (ECD) and herstatin mRNA expression were analyzed. Restriction analysis confirmed wt expression. 3T3-L1 cells exhibited HER2-ECD and herstatin mRNA expression, although at extremely low levels (compared to the control cell lines). ErbB3 cDNA was amplified using mouse mammary tumors and rat acines as positive controls. ErbB4 was not positively identified in these cells. In conclusion, this study demonstrates that 3T3-L1 cells express EGFR/erbB1, erbB2 and erbB3, and possibly, certain erbB2 splice or proteolytic variants, which are worth pursuing. |
| format |
JOUR |
| author |
Pagano, E. Fontana, V. Calvo, J.C. |
| author_facet |
Pagano, E. Fontana, V. Calvo, J.C. |
| author_sort |
Pagano, E. |
| title |
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| title_short |
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| title_full |
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| title_fullStr |
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| title_full_unstemmed |
Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes |
| title_sort |
expression of erythroblastic leukemia viral oncogene homolog (erbbs) mrnas and possible splice variants in 3t3-l1 preadipocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_17912997_v4_n5_p955_Pagano |
| work_keys_str_mv |
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| _version_ |
1807323669644443648 |