Epicuticular lipids induce aggregation in Chagas disease vectors
Background. The triatomine bugs are vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. Aggregation behavior plays an important role in their survival by facilitating the location of refuges and cohesion of aggregates, helping to keep them safely assembled int...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_17563305_v2_n1_p_Figueiras |
| Aporte de: |
| id |
todo:paper_17563305_v2_n1_p_Figueiras |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_17563305_v2_n1_p_Figueiras2023-10-03T16:32:37Z Epicuticular lipids induce aggregation in Chagas disease vectors Figueiras, A.N.L. Girotti, J.R. Mijailovsky, S.J. Juárez, P. fatty acid hexane lipid palmitic acid stearic acid animal experiment article Chagas disease controlled study disease carrier disease course lipid analysis nonhuman Triatoma infestans Hexapoda Protozoa Triatoma infestans Trypanosoma cruzi Background. The triatomine bugs are vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. Aggregation behavior plays an important role in their survival by facilitating the location of refuges and cohesion of aggregates, helping to keep them safely assembled into shelters during daylight time, when they are vulnerable to predators. There are evidences that aggregation is mediated by thigmotaxis, by volatile cues from their faeces, and by hexane-extractable contact chemoreceptive signals from their cuticle surface. The epicuticular lipids of Triatoma infestans include a complex mixture of hydrocarbons, free and esterified fatty acids, alcohols, and sterols. Results. We analyzed the response of T. infestans fifth instar nymphs after exposure to different amounts either of total epicuticular lipid extracts or individual lipid fractions. Assays were performed in a circular arena, employing a binary choice test with filter papers acting as aggregation attractive sites; papers were either impregnated with a hexane-extract of the total lipids, or lipid fraction; or with the solvent. Insects were significantly aggregated around papers impregnated with the epicuticular lipid extracts. Among the lipid fractions separately tested, only the free fatty acid fraction promoted significant bug aggregation. We also investigated the response to different amounts of selected fatty acid components of this fraction; receptiveness varied with the fatty acid chain length. No response was elicited by hexadecanoic acid (C16:0), the major fatty acid component. Octadecanoic acid (C18:0) showed a significant assembling effect in the concentration range tested (0.1 to 2 insect equivalents). The very long chain hexacosanoic acid (C26:0) was significantly attractant at low doses ( 1 equivalent), although a repellent effect was observed at higher doses. Conclusion. The detection of contact aggregation pheromones has practical application in Chagas disease vector control. These data may be used to help design new tools against triatomine bugs. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17563305_v2_n1_p_Figueiras |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
fatty acid hexane lipid palmitic acid stearic acid animal experiment article Chagas disease controlled study disease carrier disease course lipid analysis nonhuman Triatoma infestans Hexapoda Protozoa Triatoma infestans Trypanosoma cruzi |
| spellingShingle |
fatty acid hexane lipid palmitic acid stearic acid animal experiment article Chagas disease controlled study disease carrier disease course lipid analysis nonhuman Triatoma infestans Hexapoda Protozoa Triatoma infestans Trypanosoma cruzi Figueiras, A.N.L. Girotti, J.R. Mijailovsky, S.J. Juárez, P. Epicuticular lipids induce aggregation in Chagas disease vectors |
| topic_facet |
fatty acid hexane lipid palmitic acid stearic acid animal experiment article Chagas disease controlled study disease carrier disease course lipid analysis nonhuman Triatoma infestans Hexapoda Protozoa Triatoma infestans Trypanosoma cruzi |
| description |
Background. The triatomine bugs are vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. Aggregation behavior plays an important role in their survival by facilitating the location of refuges and cohesion of aggregates, helping to keep them safely assembled into shelters during daylight time, when they are vulnerable to predators. There are evidences that aggregation is mediated by thigmotaxis, by volatile cues from their faeces, and by hexane-extractable contact chemoreceptive signals from their cuticle surface. The epicuticular lipids of Triatoma infestans include a complex mixture of hydrocarbons, free and esterified fatty acids, alcohols, and sterols. Results. We analyzed the response of T. infestans fifth instar nymphs after exposure to different amounts either of total epicuticular lipid extracts or individual lipid fractions. Assays were performed in a circular arena, employing a binary choice test with filter papers acting as aggregation attractive sites; papers were either impregnated with a hexane-extract of the total lipids, or lipid fraction; or with the solvent. Insects were significantly aggregated around papers impregnated with the epicuticular lipid extracts. Among the lipid fractions separately tested, only the free fatty acid fraction promoted significant bug aggregation. We also investigated the response to different amounts of selected fatty acid components of this fraction; receptiveness varied with the fatty acid chain length. No response was elicited by hexadecanoic acid (C16:0), the major fatty acid component. Octadecanoic acid (C18:0) showed a significant assembling effect in the concentration range tested (0.1 to 2 insect equivalents). The very long chain hexacosanoic acid (C26:0) was significantly attractant at low doses ( 1 equivalent), although a repellent effect was observed at higher doses. Conclusion. The detection of contact aggregation pheromones has practical application in Chagas disease vector control. These data may be used to help design new tools against triatomine bugs. |
| format |
JOUR |
| author |
Figueiras, A.N.L. Girotti, J.R. Mijailovsky, S.J. Juárez, P. |
| author_facet |
Figueiras, A.N.L. Girotti, J.R. Mijailovsky, S.J. Juárez, P. |
| author_sort |
Figueiras, A.N.L. |
| title |
Epicuticular lipids induce aggregation in Chagas disease vectors |
| title_short |
Epicuticular lipids induce aggregation in Chagas disease vectors |
| title_full |
Epicuticular lipids induce aggregation in Chagas disease vectors |
| title_fullStr |
Epicuticular lipids induce aggregation in Chagas disease vectors |
| title_full_unstemmed |
Epicuticular lipids induce aggregation in Chagas disease vectors |
| title_sort |
epicuticular lipids induce aggregation in chagas disease vectors |
| url |
http://hdl.handle.net/20.500.12110/paper_17563305_v2_n1_p_Figueiras |
| work_keys_str_mv |
AT figueirasanl epicuticularlipidsinduceaggregationinchagasdiseasevectors AT girottijr epicuticularlipidsinduceaggregationinchagasdiseasevectors AT mijailovskysj epicuticularlipidsinduceaggregationinchagasdiseasevectors AT juarezp epicuticularlipidsinduceaggregationinchagasdiseasevectors |
| _version_ |
1807322958669021184 |