Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells wer...
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todo:paper_17560500_v11_n1_p_Canizo2023-10-03T16:32:35Z Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system Canizo, J.R. Vazquez Echegaray, C. Klisch, D. Aller, J.F. Paz, D.A. Alberio, R.H. Alberio, R. Guberman, A.S. Bovine and porcine fibroblasts iPS-like cells Lentiviral vectors Reprogramming STEMCCA kruppel like factor octamer transcription factor 4 transcription factor Sox animal bovine cell differentiation fibroblast gene expression regulation human induced pluripotent stem cell Lentivirus metabolism nuclear reprogramming physiology pig Animals Cattle Cell Differentiation Cellular Reprogramming Fibroblasts Gene Expression Regulation Humans Induced Pluripotent Stem Cells Kruppel-Like Transcription Factors Lentivirus Octamer Transcription Factor-3 SOXB1 Transcription Factors Swine Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells. © 2018 The Author(s). JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17560500_v11_n1_p_Canizo |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bovine and porcine fibroblasts iPS-like cells Lentiviral vectors Reprogramming STEMCCA kruppel like factor octamer transcription factor 4 transcription factor Sox animal bovine cell differentiation fibroblast gene expression regulation human induced pluripotent stem cell Lentivirus metabolism nuclear reprogramming physiology pig Animals Cattle Cell Differentiation Cellular Reprogramming Fibroblasts Gene Expression Regulation Humans Induced Pluripotent Stem Cells Kruppel-Like Transcription Factors Lentivirus Octamer Transcription Factor-3 SOXB1 Transcription Factors Swine |
| spellingShingle |
Bovine and porcine fibroblasts iPS-like cells Lentiviral vectors Reprogramming STEMCCA kruppel like factor octamer transcription factor 4 transcription factor Sox animal bovine cell differentiation fibroblast gene expression regulation human induced pluripotent stem cell Lentivirus metabolism nuclear reprogramming physiology pig Animals Cattle Cell Differentiation Cellular Reprogramming Fibroblasts Gene Expression Regulation Humans Induced Pluripotent Stem Cells Kruppel-Like Transcription Factors Lentivirus Octamer Transcription Factor-3 SOXB1 Transcription Factors Swine Canizo, J.R. Vazquez Echegaray, C. Klisch, D. Aller, J.F. Paz, D.A. Alberio, R.H. Alberio, R. Guberman, A.S. Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| topic_facet |
Bovine and porcine fibroblasts iPS-like cells Lentiviral vectors Reprogramming STEMCCA kruppel like factor octamer transcription factor 4 transcription factor Sox animal bovine cell differentiation fibroblast gene expression regulation human induced pluripotent stem cell Lentivirus metabolism nuclear reprogramming physiology pig Animals Cattle Cell Differentiation Cellular Reprogramming Fibroblasts Gene Expression Regulation Humans Induced Pluripotent Stem Cells Kruppel-Like Transcription Factors Lentivirus Octamer Transcription Factor-3 SOXB1 Transcription Factors Swine |
| description |
Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells. © 2018 The Author(s). |
| format |
JOUR |
| author |
Canizo, J.R. Vazquez Echegaray, C. Klisch, D. Aller, J.F. Paz, D.A. Alberio, R.H. Alberio, R. Guberman, A.S. |
| author_facet |
Canizo, J.R. Vazquez Echegaray, C. Klisch, D. Aller, J.F. Paz, D.A. Alberio, R.H. Alberio, R. Guberman, A.S. |
| author_sort |
Canizo, J.R. |
| title |
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_short |
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_full |
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_fullStr |
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_full_unstemmed |
Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system |
| title_sort |
exogenous human oksm factors maintain pluripotency gene expression of bovine and porcine ips-like cells obtained with stemcca delivery system |
| url |
http://hdl.handle.net/20.500.12110/paper_17560500_v11_n1_p_Canizo |
| work_keys_str_mv |
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