Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)

Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to subleth...

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Autores principales: Bernal-Rey, D.L., Dos Santos Afonso, M., Menendez-Helman, R.J.
Formato: JOUR
Materias:
Acetylcholinesterase
AChE
Biomarkers
Chlorpyrifos
Cnesterodon decemmaculatus
Freshwater fish
Gambusia affinis
Pesticides
Teleost.
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_17434955_v8_n4_p316_BernalRey
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_17434955_v8_n4_p316_BernalRey
record_format dspace
spelling todo:paper_17434955_v8_n4_p316_BernalRey2023-10-03T16:31:22Z Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis) Bernal-Rey, D.L. Dos Santos Afonso, M. Menendez-Helman, R.J. Acetylcholinesterase AChE Biomarkers Chlorpyrifos Cnesterodon decemmaculatus Freshwater fish Gambusia affinis Pesticides Teleost. Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to sublethal concentrations (1 and 5 μg CPF × L-1) or remained in control water for 96 h. AChE activity was inhibited 48% and 69% in C. decemmaculatus and 27% and 36% in G. affinis for 1 and 5 μg CPF × L-1, respectively. In vitro assays showed no differential sensitivity of the enzyme to CPF between species. However, the IC50 for in vitro exposure to chlorpyrifos-oxon (CPF-oxon, a product of the natural oxidative desulfuration of CPF) were 10.2 nM and 54.2 nM for C. decemmaculatus and G. affinis, respectively. Therefore, a different sensitivity of the enzyme between both species could explain the dissimilar behaviour of AChE after in vivo exposure to CPF. Copyright © 2017 Inderscience Enterprises Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_17434955_v8_n4_p316_BernalRey
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Acetylcholinesterase
AChE
Biomarkers
Chlorpyrifos
Cnesterodon decemmaculatus
Freshwater fish
Gambusia affinis
Pesticides
Teleost.
spellingShingle Acetylcholinesterase
AChE
Biomarkers
Chlorpyrifos
Cnesterodon decemmaculatus
Freshwater fish
Gambusia affinis
Pesticides
Teleost.
Bernal-Rey, D.L.
Dos Santos Afonso, M.
Menendez-Helman, R.J.
Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
topic_facet Acetylcholinesterase
AChE
Biomarkers
Chlorpyrifos
Cnesterodon decemmaculatus
Freshwater fish
Gambusia affinis
Pesticides
Teleost.
description Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to sublethal concentrations (1 and 5 μg CPF × L-1) or remained in control water for 96 h. AChE activity was inhibited 48% and 69% in C. decemmaculatus and 27% and 36% in G. affinis for 1 and 5 μg CPF × L-1, respectively. In vitro assays showed no differential sensitivity of the enzyme to CPF between species. However, the IC50 for in vitro exposure to chlorpyrifos-oxon (CPF-oxon, a product of the natural oxidative desulfuration of CPF) were 10.2 nM and 54.2 nM for C. decemmaculatus and G. affinis, respectively. Therefore, a different sensitivity of the enzyme between both species could explain the dissimilar behaviour of AChE after in vivo exposure to CPF. Copyright © 2017 Inderscience Enterprises Ltd.
format JOUR
author Bernal-Rey, D.L.
Dos Santos Afonso, M.
Menendez-Helman, R.J.
author_facet Bernal-Rey, D.L.
Dos Santos Afonso, M.
Menendez-Helman, R.J.
author_sort Bernal-Rey, D.L.
title Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
title_short Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
title_full Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
title_fullStr Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
title_full_unstemmed Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
title_sort effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (cnesterodon decemmaculatus and gambusia affinis)
url http://hdl.handle.net/20.500.12110/paper_17434955_v8_n4_p316_BernalRey
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AT dossantosafonsom effectsofchlorpyrifosonacetylcholinesteraseactivityintwofreshwaterfishspeciescnesterodondecemmaculatusandgambusiaaffinis
AT menendezhelmanrj effectsofchlorpyrifosonacetylcholinesteraseactivityintwofreshwaterfishspeciescnesterodondecemmaculatusandgambusiaaffinis
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