Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In t...
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| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_16789199_v15_n1_p28_Bustillo |
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todo:paper_16789199_v15_n1_p28_Bustillo2023-10-03T16:29:42Z Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina Bustillo, S. Lucero, H. Leiva, L.C. Acosta, O. Kier Joffé, E.B. Gorodner, J.O. Apoptosis Bothrops alternatus Bothrops diporus C2C12 Cytotoxicity acridine orange Bothrops alternatus venom Bothrops diporus venom ethidium bromide fluorescent dye snake venom unclassified drug animal cell apoptosis Argentina article cell death cell structure cell viability concentration (parameters) controlled study cytotoxicity mouse muscle cell myoblast nonhuman phase contrast microscopy skeletal muscle snake Bothrops Bothrops alternatus Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 μg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC 50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC 50: 2 μg/mL) than B. alternatus (CC 50: 5.8 μg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16789199_v15_n1_p28_Bustillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Apoptosis Bothrops alternatus Bothrops diporus C2C12 Cytotoxicity acridine orange Bothrops alternatus venom Bothrops diporus venom ethidium bromide fluorescent dye snake venom unclassified drug animal cell apoptosis Argentina article cell death cell structure cell viability concentration (parameters) controlled study cytotoxicity mouse muscle cell myoblast nonhuman phase contrast microscopy skeletal muscle snake Bothrops Bothrops alternatus |
| spellingShingle |
Apoptosis Bothrops alternatus Bothrops diporus C2C12 Cytotoxicity acridine orange Bothrops alternatus venom Bothrops diporus venom ethidium bromide fluorescent dye snake venom unclassified drug animal cell apoptosis Argentina article cell death cell structure cell viability concentration (parameters) controlled study cytotoxicity mouse muscle cell myoblast nonhuman phase contrast microscopy skeletal muscle snake Bothrops Bothrops alternatus Bustillo, S. Lucero, H. Leiva, L.C. Acosta, O. Kier Joffé, E.B. Gorodner, J.O. Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| topic_facet |
Apoptosis Bothrops alternatus Bothrops diporus C2C12 Cytotoxicity acridine orange Bothrops alternatus venom Bothrops diporus venom ethidium bromide fluorescent dye snake venom unclassified drug animal cell apoptosis Argentina article cell death cell structure cell viability concentration (parameters) controlled study cytotoxicity mouse muscle cell myoblast nonhuman phase contrast microscopy skeletal muscle snake Bothrops Bothrops alternatus |
| description |
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 μg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC 50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC 50: 2 μg/mL) than B. alternatus (CC 50: 5.8 μg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line. |
| format |
JOUR |
| author |
Bustillo, S. Lucero, H. Leiva, L.C. Acosta, O. Kier Joffé, E.B. Gorodner, J.O. |
| author_facet |
Bustillo, S. Lucero, H. Leiva, L.C. Acosta, O. Kier Joffé, E.B. Gorodner, J.O. |
| author_sort |
Bustillo, S. |
| title |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| title_short |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| title_full |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| title_fullStr |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| title_full_unstemmed |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
| title_sort |
cytotoxicity and morphological analysis of cell death induced by bothrops venoms from the northeast of argentina |
| url |
http://hdl.handle.net/20.500.12110/paper_16789199_v15_n1_p28_Bustillo |
| work_keys_str_mv |
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