Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection.
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_16765680_v4_n4_p675_Nieves |
| Aporte de: |
| id |
todo:paper_16765680_v4_n4_p675_Nieves |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_16765680_v4_n4_p675_Nieves2023-10-03T16:29:39Z Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. Nieves, M. Mühlmann, M. Mudry, M.D. animal article Cebidae chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin male methodology microdissection molecular evolution polymerase chain reaction sensitivity and specificity Animals Cebus Chromosome Banding Evolution, Molecular Female Heterochromatin In Situ Hybridization, Fluorescence Male Microdissection Polymerase Chain Reaction Sensitivity and Specificity Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a FISH probe of the chromosome 11 heterochromatic block (11qHe+) of Cebus apella paraguayanus (CAPp). Fragments of the 11qHe+ microdissected from fibroblast cell culture were collected in a PCR tube, amplified by degenerate oligonucleotide primer-PCR and subsequently labeled. The specificity of the FISH probe was confirmed in metaphases of some Ceboidea species. Signals were located in the He+ of chromosomes 4, 11, 12, 13, and 19 of CAPp and in the He+ of chromosomes 4, 12 and 13 of C. a. nigritus (CAPn); no signals were observed when other Ceboidea species were analyzed. We propose that the heterochromatin observed in CAPp and CAPn is specific for these species. We consider this C. apella heterochromatin identity as a possible key for the interpretation of chromosomal evolution in these Ceboidea. Fil:Nieves, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16765680_v4_n4_p675_Nieves |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
animal article Cebidae chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin male methodology microdissection molecular evolution polymerase chain reaction sensitivity and specificity Animals Cebus Chromosome Banding Evolution, Molecular Female Heterochromatin In Situ Hybridization, Fluorescence Male Microdissection Polymerase Chain Reaction Sensitivity and Specificity |
| spellingShingle |
animal article Cebidae chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin male methodology microdissection molecular evolution polymerase chain reaction sensitivity and specificity Animals Cebus Chromosome Banding Evolution, Molecular Female Heterochromatin In Situ Hybridization, Fluorescence Male Microdissection Polymerase Chain Reaction Sensitivity and Specificity Nieves, M. Mühlmann, M. Mudry, M.D. Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| topic_facet |
animal article Cebidae chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin male methodology microdissection molecular evolution polymerase chain reaction sensitivity and specificity Animals Cebus Chromosome Banding Evolution, Molecular Female Heterochromatin In Situ Hybridization, Fluorescence Male Microdissection Polymerase Chain Reaction Sensitivity and Specificity |
| description |
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a FISH probe of the chromosome 11 heterochromatic block (11qHe+) of Cebus apella paraguayanus (CAPp). Fragments of the 11qHe+ microdissected from fibroblast cell culture were collected in a PCR tube, amplified by degenerate oligonucleotide primer-PCR and subsequently labeled. The specificity of the FISH probe was confirmed in metaphases of some Ceboidea species. Signals were located in the He+ of chromosomes 4, 11, 12, 13, and 19 of CAPp and in the He+ of chromosomes 4, 12 and 13 of C. a. nigritus (CAPn); no signals were observed when other Ceboidea species were analyzed. We propose that the heterochromatin observed in CAPp and CAPn is specific for these species. We consider this C. apella heterochromatin identity as a possible key for the interpretation of chromosomal evolution in these Ceboidea. |
| format |
JOUR |
| author |
Nieves, M. Mühlmann, M. Mudry, M.D. |
| author_facet |
Nieves, M. Mühlmann, M. Mudry, M.D. |
| author_sort |
Nieves, M. |
| title |
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| title_short |
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| title_full |
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| title_fullStr |
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| title_full_unstemmed |
Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| title_sort |
heterochromatin and chromosome evolution: a fish probe of cebus apella paraguayanus (primate: platyrrhini) developed by chromosome microdissection. |
| url |
http://hdl.handle.net/20.500.12110/paper_16765680_v4_n4_p675_Nieves |
| work_keys_str_mv |
AT nievesm heterochromatinandchromosomeevolutionafishprobeofcebusapellaparaguayanusprimateplatyrrhinidevelopedbychromosomemicrodissection AT muhlmannm heterochromatinandchromosomeevolutionafishprobeofcebusapellaparaguayanusprimateplatyrrhinidevelopedbychromosomemicrodissection AT mudrymd heterochromatinandchromosomeevolutionafishprobeofcebusapellaparaguayanusprimateplatyrrhinidevelopedbychromosomemicrodissection |
| _version_ |
1807322004753219584 |