Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect

Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM an...

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Autores principales:	Rubio, A.P.D., Martínez, J.H., Casillas, D.C.M., Leskow, F.C., Piuri, M., Pérez, O.E.
Formato:	JOUR
Materias:	CFSE Lactobacillus casei BL23 Microvesicles Probiotics Proteomics Vesicle size distribution adhesin bacterial enzyme bacterial protein capsid protein cold shock protein DNA heat shock protein probiotic agent proteinase RNA secretory protein structural protein viral protein Article atomic force microscopy bacterial strain bacterium culture confocal laser scanning microscopy dispersity functional food Lactobacillus casei light scattering membrane microparticle membrane potential nonhuman particle size polyacrylamide gel electrophoresis protein function proteomics surface charge transmission electron microscopy
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1664302X_v8_nSEP_p_Rubio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1664302X_v8_nSEP_p_Rubio
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spelling	todo:paper_1664302X_v8_nSEP_p_Rubio2023-10-03T16:29:06Z Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect Rubio, A.P.D. Martínez, J.H. Casillas, D.C.M. Leskow, F.C. Piuri, M. Pérez, O.E. CFSE Lactobacillus casei BL23 Microvesicles Probiotics Proteomics Vesicle size distribution adhesin bacterial enzyme bacterial protein capsid protein cold shock protein DNA heat shock protein probiotic agent proteinase RNA secretory protein structural protein viral protein Article atomic force microscopy bacterial strain bacterium culture confocal laser scanning microscopy dispersity functional food Lactobacillus casei Lactobacillus casei BL23 light scattering membrane microparticle membrane potential nonhuman particle size polyacrylamide gel electrophoresis protein function proteomics surface charge transmission electron microscopy Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30-50 nm). MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS), we identified a total of 103 proteins; 13 exclusively present in the MVs. The MVs content included cell envelope associated and secretory proteins, heat and cold shock proteins, several metabolic enzymes, proteases, structural components of the ribosome, membrane transporters, cell wall-associated hydrolases and phage related proteins. In particular, we identified proteins described as mediators of Lactobacillus' probiotic effects such as p40, p75 and the product of LCABL_31160, annotated as an adhesion protein. The presence of these proteins suggests a role for the MVs in the bacteria-gastrointestinal cells interface. The expression and further encapsulation of proteins into MVs of GRAS (Generally Recognized as Safe) bacteria could represent a scientific novelty, with applications in food, nutraceuticals and clinical therapies. © 2017 Domínguez Rubio, Martínez, Martínez Casillas, Coluccio Leskow, Piuri and Pérez. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1664302X_v8_nSEP_p_Rubio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	CFSE Lactobacillus casei BL23 Microvesicles Probiotics Proteomics Vesicle size distribution adhesin bacterial enzyme bacterial protein capsid protein cold shock protein DNA heat shock protein probiotic agent proteinase RNA secretory protein structural protein viral protein Article atomic force microscopy bacterial strain bacterium culture confocal laser scanning microscopy dispersity functional food Lactobacillus casei Lactobacillus casei BL23 light scattering membrane microparticle membrane potential nonhuman particle size polyacrylamide gel electrophoresis protein function proteomics surface charge transmission electron microscopy
spellingShingle	CFSE Lactobacillus casei BL23 Microvesicles Probiotics Proteomics Vesicle size distribution adhesin bacterial enzyme bacterial protein capsid protein cold shock protein DNA heat shock protein probiotic agent proteinase RNA secretory protein structural protein viral protein Article atomic force microscopy bacterial strain bacterium culture confocal laser scanning microscopy dispersity functional food Lactobacillus casei Lactobacillus casei BL23 light scattering membrane microparticle membrane potential nonhuman particle size polyacrylamide gel electrophoresis protein function proteomics surface charge transmission electron microscopy Rubio, A.P.D. Martínez, J.H. Casillas, D.C.M. Leskow, F.C. Piuri, M. Pérez, O.E. Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
topic_facet	CFSE Lactobacillus casei BL23 Microvesicles Probiotics Proteomics Vesicle size distribution adhesin bacterial enzyme bacterial protein capsid protein cold shock protein DNA heat shock protein probiotic agent proteinase RNA secretory protein structural protein viral protein Article atomic force microscopy bacterial strain bacterium culture confocal laser scanning microscopy dispersity functional food Lactobacillus casei Lactobacillus casei BL23 light scattering membrane microparticle membrane potential nonhuman particle size polyacrylamide gel electrophoresis protein function proteomics surface charge transmission electron microscopy
description	Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30-50 nm). MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS), we identified a total of 103 proteins; 13 exclusively present in the MVs. The MVs content included cell envelope associated and secretory proteins, heat and cold shock proteins, several metabolic enzymes, proteases, structural components of the ribosome, membrane transporters, cell wall-associated hydrolases and phage related proteins. In particular, we identified proteins described as mediators of Lactobacillus' probiotic effects such as p40, p75 and the product of LCABL_31160, annotated as an adhesion protein. The presence of these proteins suggests a role for the MVs in the bacteria-gastrointestinal cells interface. The expression and further encapsulation of proteins into MVs of GRAS (Generally Recognized as Safe) bacteria could represent a scientific novelty, with applications in food, nutraceuticals and clinical therapies. © 2017 Domínguez Rubio, Martínez, Martínez Casillas, Coluccio Leskow, Piuri and Pérez.
format	JOUR
author	Rubio, A.P.D. Martínez, J.H. Casillas, D.C.M. Leskow, F.C. Piuri, M. Pérez, O.E.
author_facet	Rubio, A.P.D. Martínez, J.H. Casillas, D.C.M. Leskow, F.C. Piuri, M. Pérez, O.E.
author_sort	Rubio, A.P.D.
title	Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
title_short	Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
title_full	Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
title_fullStr	Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
title_full_unstemmed	Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect
title_sort	lactobacillus casei bl23 produces microvesicles carrying proteins that have been associated with its probiotic effect
url	http://hdl.handle.net/20.500.12110/paper_1664302X_v8_nSEP_p_Rubio
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Lactobacillus casei BL23 produces microvesicles carrying proteins that have been associated with its probiotic effect

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