Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves
Hypoxia in freshwater ecosystems is spreading as a consequence of global change, including pollution and eutrophication. In the Patagonian Andes, a decline in precipitation causes reduced lake water volumes and stagnant conditions that limit oxygen transport and exacerbate hypoxia below the upper mi...
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todo:paper_1664042X_v9_nFEB_p_Yusseppone2023-10-03T16:28:59Z Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves Yusseppone, M.S. Rocchetta, I. Sabatini, S.E. Luquet, C.M. de Molina, M.C.R. Held, C. Abele, D. Alternative oxidase Anaerobiosis Diplodon chilensis Hypoxia Mitochondrial electron transport Oxidative stress alternative oxidase caspase 3 caspase 7 glutathione peroxidase glycogen phosphorylase malate dehydrogenase oxidoreductase succinate dehydrogenase succinic acid unclassified drug animal experiment animal tissue anoxia apoptosis Argentina Article biochemistry bivalve controlled study Diplodon chilensis electron transport enzyme activity enzyme analysis enzyme induction enzyme regulation freshwater species gene expression regulation gill mantle (mollusc) metabolic inhibition mitochondrial energy transfer nonhuman oxidative stress physical tolerance protein carbonylation protein expression survival upregulation Hypoxia in freshwater ecosystems is spreading as a consequence of global change, including pollution and eutrophication. In the Patagonian Andes, a decline in precipitation causes reduced lake water volumes and stagnant conditions that limit oxygen transport and exacerbate hypoxia below the upper mixed layer. We analyzed the molecular and biochemical response of the North Patagonian bivalve Diplodon chilensis after 10 days of experimental anoxia (< 0.2 mg O2/L), hypoxia (2 mg O2/L), and normoxia (9 mg O2/L). Specifically, we investigated the expression of an alternative oxidase (AOX) pathway assumed to shortcut the regular mitochondrial electron transport system (ETS) during metabolic rate depression (MRD) in hypoxia-tolerant invertebrates. Whereas, the AOX system was strongly upregulated during anoxia in gills, ETS activities and energy mobilization decreased [less transcription of glycogen phosphorylase (GlyP) and succinate dehydrogenase (SDH) in gills and mantle]. Accumulation of succinate and induction of malate dehydrogenase (MDH) activity could indicate activation of anaerobic mitochondrial pathways to support anoxic survival in D. chilensis. Oxidative stress [protein carbonylation, glutathione peroxidase (GPx) expression] and apoptotic intensity (caspase 3/7 activity) decreased, whereas an unfolded protein response (HSP90) was induced under anoxia. This is the first clear evidence of the concerted regulation of the AOX and ETS genes in a hypoxia-tolerant freshwater bivalve and yet another example that exposure to hypoxia and anoxia is not necessarily accompanied by oxidative stress in hypoxia-tolerant mollusks. © 2018 Yusseppone, Rocchetta, Sabatini, Luquet, Ríos de Molina, Held and Abele. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1664042X_v9_nFEB_p_Yusseppone |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alternative oxidase Anaerobiosis Diplodon chilensis Hypoxia Mitochondrial electron transport Oxidative stress alternative oxidase caspase 3 caspase 7 glutathione peroxidase glycogen phosphorylase malate dehydrogenase oxidoreductase succinate dehydrogenase succinic acid unclassified drug animal experiment animal tissue anoxia apoptosis Argentina Article biochemistry bivalve controlled study Diplodon chilensis electron transport enzyme activity enzyme analysis enzyme induction enzyme regulation freshwater species gene expression regulation gill mantle (mollusc) metabolic inhibition mitochondrial energy transfer nonhuman oxidative stress physical tolerance protein carbonylation protein expression survival upregulation |
| spellingShingle |
Alternative oxidase Anaerobiosis Diplodon chilensis Hypoxia Mitochondrial electron transport Oxidative stress alternative oxidase caspase 3 caspase 7 glutathione peroxidase glycogen phosphorylase malate dehydrogenase oxidoreductase succinate dehydrogenase succinic acid unclassified drug animal experiment animal tissue anoxia apoptosis Argentina Article biochemistry bivalve controlled study Diplodon chilensis electron transport enzyme activity enzyme analysis enzyme induction enzyme regulation freshwater species gene expression regulation gill mantle (mollusc) metabolic inhibition mitochondrial energy transfer nonhuman oxidative stress physical tolerance protein carbonylation protein expression survival upregulation Yusseppone, M.S. Rocchetta, I. Sabatini, S.E. Luquet, C.M. de Molina, M.C.R. Held, C. Abele, D. Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| topic_facet |
Alternative oxidase Anaerobiosis Diplodon chilensis Hypoxia Mitochondrial electron transport Oxidative stress alternative oxidase caspase 3 caspase 7 glutathione peroxidase glycogen phosphorylase malate dehydrogenase oxidoreductase succinate dehydrogenase succinic acid unclassified drug animal experiment animal tissue anoxia apoptosis Argentina Article biochemistry bivalve controlled study Diplodon chilensis electron transport enzyme activity enzyme analysis enzyme induction enzyme regulation freshwater species gene expression regulation gill mantle (mollusc) metabolic inhibition mitochondrial energy transfer nonhuman oxidative stress physical tolerance protein carbonylation protein expression survival upregulation |
| description |
Hypoxia in freshwater ecosystems is spreading as a consequence of global change, including pollution and eutrophication. In the Patagonian Andes, a decline in precipitation causes reduced lake water volumes and stagnant conditions that limit oxygen transport and exacerbate hypoxia below the upper mixed layer. We analyzed the molecular and biochemical response of the North Patagonian bivalve Diplodon chilensis after 10 days of experimental anoxia (< 0.2 mg O2/L), hypoxia (2 mg O2/L), and normoxia (9 mg O2/L). Specifically, we investigated the expression of an alternative oxidase (AOX) pathway assumed to shortcut the regular mitochondrial electron transport system (ETS) during metabolic rate depression (MRD) in hypoxia-tolerant invertebrates. Whereas, the AOX system was strongly upregulated during anoxia in gills, ETS activities and energy mobilization decreased [less transcription of glycogen phosphorylase (GlyP) and succinate dehydrogenase (SDH) in gills and mantle]. Accumulation of succinate and induction of malate dehydrogenase (MDH) activity could indicate activation of anaerobic mitochondrial pathways to support anoxic survival in D. chilensis. Oxidative stress [protein carbonylation, glutathione peroxidase (GPx) expression] and apoptotic intensity (caspase 3/7 activity) decreased, whereas an unfolded protein response (HSP90) was induced under anoxia. This is the first clear evidence of the concerted regulation of the AOX and ETS genes in a hypoxia-tolerant freshwater bivalve and yet another example that exposure to hypoxia and anoxia is not necessarily accompanied by oxidative stress in hypoxia-tolerant mollusks. © 2018 Yusseppone, Rocchetta, Sabatini, Luquet, Ríos de Molina, Held and Abele. |
| format |
JOUR |
| author |
Yusseppone, M.S. Rocchetta, I. Sabatini, S.E. Luquet, C.M. de Molina, M.C.R. Held, C. Abele, D. |
| author_facet |
Yusseppone, M.S. Rocchetta, I. Sabatini, S.E. Luquet, C.M. de Molina, M.C.R. Held, C. Abele, D. |
| author_sort |
Yusseppone, M.S. |
| title |
Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| title_short |
Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| title_full |
Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| title_fullStr |
Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| title_full_unstemmed |
Inducing the alternative Oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| title_sort |
inducing the alternative oxidase forms part of the molecular strategy of anoxic survival in freshwater bivalves |
| url |
http://hdl.handle.net/20.500.12110/paper_1664042X_v9_nFEB_p_Yusseppone |
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