The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner
The barley plastome mutant CL2 (cytoplasmic line 2) carries a point mutation in the infA gene, a homologue of the bacterial gene for the conserved translation initiator factor 1 (IF1). The function of infA in plastids is not known. The mutation in CL2 leads to a temporal chlorophyll deficiency in th...
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todo:paper_16174615_v279_n4_p403_Colombo2023-10-03T16:28:27Z The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner Colombo, N. Emanuel, C. Lainez, V. Maldonado, S. Prina, A.R. Börner, T. Chloroplast transcription Chloroplast translation Hordeum vulgare infA Plastid signal chlorophyll initiation factor 1 ribosome RNA RNA polymerase article bacterial gene bacteriophage typing barley cell aging chloroplast cytoplasmic line 2 gene expression genetic transcription housekeeping gene leaf lamina mutant nonhuman phenotype plastid point mutation priority journal wild type Base Sequence Cell Aging Cell Nucleus Chloroplasts DNA Primers DNA-Directed RNA Polymerases Eukaryotic Initiation Factors Genes, Plant Genome, Chloroplast Hordeum Microscopy, Electron, Transmission Models, Biological Phenotype Plant Proteins Point Mutation Transcription, Genetic Bacteria (microorganisms) Hordeum Hordeum vulgare The barley plastome mutant CL2 (cytoplasmic line 2) carries a point mutation in the infA gene, a homologue of the bacterial gene for the conserved translation initiator factor 1 (IF1). The function of infA in plastids is not known. The mutation in CL2 leads to a temporal chlorophyll deficiency in the primary leaf blade that is normalised in the basal and middle parts during further development. We have compared the expression of selected nuclear and plastid genes in different parts of primary leaves of CL2 and wild-type and found no indication for an adverse effect of the mutation on plastidial transcription. We observed an enhanced expression of RpoTp (encoding the phage-type nuclear-encoded plastid RNA polymerase) suggested to be caused by retrograde plastid signalling. Decreased amounts of plastid rRNA in basal and top sections are in agreement with the idea that the mutation in infA leads to a time- and position-dependent defect of plastid translation that causes a delay in plastid development. The normalisation of the phenotype in the middle section of CL2 leaves correlates with wild-type levels of chloroplast 16S rRNA and RbcL and increased expression of plastid housekeeping genes. The normalisation was not observed in cells at the tip of CL2 leaves suggesting different ways of regulating chloroplast development in cells at the tip of primary barley leaves as compared with other leaf sections. © 2008 Springer-Verlag. Fil:Colombo, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lainez, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16174615_v279_n4_p403_Colombo |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chloroplast transcription Chloroplast translation Hordeum vulgare infA Plastid signal chlorophyll initiation factor 1 ribosome RNA RNA polymerase article bacterial gene bacteriophage typing barley cell aging chloroplast cytoplasmic line 2 gene expression genetic transcription housekeeping gene leaf lamina mutant nonhuman phenotype plastid point mutation priority journal wild type Base Sequence Cell Aging Cell Nucleus Chloroplasts DNA Primers DNA-Directed RNA Polymerases Eukaryotic Initiation Factors Genes, Plant Genome, Chloroplast Hordeum Microscopy, Electron, Transmission Models, Biological Phenotype Plant Proteins Point Mutation Transcription, Genetic Bacteria (microorganisms) Hordeum Hordeum vulgare |
| spellingShingle |
Chloroplast transcription Chloroplast translation Hordeum vulgare infA Plastid signal chlorophyll initiation factor 1 ribosome RNA RNA polymerase article bacterial gene bacteriophage typing barley cell aging chloroplast cytoplasmic line 2 gene expression genetic transcription housekeeping gene leaf lamina mutant nonhuman phenotype plastid point mutation priority journal wild type Base Sequence Cell Aging Cell Nucleus Chloroplasts DNA Primers DNA-Directed RNA Polymerases Eukaryotic Initiation Factors Genes, Plant Genome, Chloroplast Hordeum Microscopy, Electron, Transmission Models, Biological Phenotype Plant Proteins Point Mutation Transcription, Genetic Bacteria (microorganisms) Hordeum Hordeum vulgare Colombo, N. Emanuel, C. Lainez, V. Maldonado, S. Prina, A.R. Börner, T. The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| topic_facet |
Chloroplast transcription Chloroplast translation Hordeum vulgare infA Plastid signal chlorophyll initiation factor 1 ribosome RNA RNA polymerase article bacterial gene bacteriophage typing barley cell aging chloroplast cytoplasmic line 2 gene expression genetic transcription housekeeping gene leaf lamina mutant nonhuman phenotype plastid point mutation priority journal wild type Base Sequence Cell Aging Cell Nucleus Chloroplasts DNA Primers DNA-Directed RNA Polymerases Eukaryotic Initiation Factors Genes, Plant Genome, Chloroplast Hordeum Microscopy, Electron, Transmission Models, Biological Phenotype Plant Proteins Point Mutation Transcription, Genetic Bacteria (microorganisms) Hordeum Hordeum vulgare |
| description |
The barley plastome mutant CL2 (cytoplasmic line 2) carries a point mutation in the infA gene, a homologue of the bacterial gene for the conserved translation initiator factor 1 (IF1). The function of infA in plastids is not known. The mutation in CL2 leads to a temporal chlorophyll deficiency in the primary leaf blade that is normalised in the basal and middle parts during further development. We have compared the expression of selected nuclear and plastid genes in different parts of primary leaves of CL2 and wild-type and found no indication for an adverse effect of the mutation on plastidial transcription. We observed an enhanced expression of RpoTp (encoding the phage-type nuclear-encoded plastid RNA polymerase) suggested to be caused by retrograde plastid signalling. Decreased amounts of plastid rRNA in basal and top sections are in agreement with the idea that the mutation in infA leads to a time- and position-dependent defect of plastid translation that causes a delay in plastid development. The normalisation of the phenotype in the middle section of CL2 leaves correlates with wild-type levels of chloroplast 16S rRNA and RbcL and increased expression of plastid housekeeping genes. The normalisation was not observed in cells at the tip of CL2 leaves suggesting different ways of regulating chloroplast development in cells at the tip of primary barley leaves as compared with other leaf sections. © 2008 Springer-Verlag. |
| format |
JOUR |
| author |
Colombo, N. Emanuel, C. Lainez, V. Maldonado, S. Prina, A.R. Börner, T. |
| author_facet |
Colombo, N. Emanuel, C. Lainez, V. Maldonado, S. Prina, A.R. Börner, T. |
| author_sort |
Colombo, N. |
| title |
The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| title_short |
The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| title_full |
The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| title_fullStr |
The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| title_full_unstemmed |
The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| title_sort |
barley plastome mutant cl2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner |
| url |
http://hdl.handle.net/20.500.12110/paper_16174615_v279_n4_p403_Colombo |
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