Photooxidation of pterin in aqueous solutions: Biological and biomedical implications

Studies of the photochemical reactivity of pterin (-2-aminopteridin-4(3H)- one; PT) in acidic (pH 5.0 -6.0) and alkaline (pH 10.2 - 10.8) aqueous solutions have been performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin layer chromatography (TLC), high-performance li...

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Autores principales:	Cabrerizo, F.M., Dantola, M.L., Thomas, A.H., Lorente, C., Braun, A.M., Oliveros, E., Capparelli, A.L.
Formato:	JOUR
Materias:	pterin derivative water article metabolism oxidation reduction reaction photochemistry radiation exposure solution and solubility ultraviolet radiation Oxidation-Reduction Photochemistry Pterins Solutions Ultraviolet Rays Water
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_16121872_v1_n11_p1800_Cabrerizo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_16121872_v1_n11_p1800_Cabrerizo2023-10-03T16:28:00Z Photooxidation of pterin in aqueous solutions: Biological and biomedical implications Cabrerizo, F.M. Dantola, M.L. Thomas, A.H. Lorente, C. Braun, A.M. Oliveros, E. Capparelli, A.L. pterin derivative water article metabolism oxidation reduction reaction photochemistry radiation exposure solution and solubility ultraviolet radiation Oxidation-Reduction Photochemistry Pterins Solutions Ultraviolet Rays Water Studies of the photochemical reactivity of pterin (-2-aminopteridin-4(3H)- one; PT) in acidic (pH 5.0 -6.0) and alkaline (pH 10.2 - 10.8) aqueous solutions have been performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O2 determination. PT is not light-sensitive in the absence of molecular oxygen, but it undergoes photooxidation in the presence of O2 , yielding several nonpteridinic products. The quantum yields for PT disappearance were found to be 8.2 (-0.6)-10-4 and 1.2 (-0.2)-10-3 in acidic and alkaline media, respectively. H2O2 was detected and quantified in irradiated solutions of PT; and its importance from a biomedical point of view is discussed. The rate constant of the chemical reaction between singlet oxygen (1O2) and PT was determined to be 2.5 (-0.2)-105 l mol-1 s-1 in alkaline medium, and the role of 1O2 in the photooxidation of pterin was evaluated. © 2004 Verlag Helvetica Chimica Acta AG, Zürich. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16121872_v1_n11_p1800_Cabrerizo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	pterin derivative water article metabolism oxidation reduction reaction photochemistry radiation exposure solution and solubility ultraviolet radiation Oxidation-Reduction Photochemistry Pterins Solutions Ultraviolet Rays Water
spellingShingle	pterin derivative water article metabolism oxidation reduction reaction photochemistry radiation exposure solution and solubility ultraviolet radiation Oxidation-Reduction Photochemistry Pterins Solutions Ultraviolet Rays Water Cabrerizo, F.M. Dantola, M.L. Thomas, A.H. Lorente, C. Braun, A.M. Oliveros, E. Capparelli, A.L. Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
topic_facet	pterin derivative water article metabolism oxidation reduction reaction photochemistry radiation exposure solution and solubility ultraviolet radiation Oxidation-Reduction Photochemistry Pterins Solutions Ultraviolet Rays Water
description	Studies of the photochemical reactivity of pterin (-2-aminopteridin-4(3H)- one; PT) in acidic (pH 5.0 -6.0) and alkaline (pH 10.2 - 10.8) aqueous solutions have been performed. The photochemical reactions were followed by UV/VIS spectrophotometry, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), and an enzymatic method for H2O2 determination. PT is not light-sensitive in the absence of molecular oxygen, but it undergoes photooxidation in the presence of O2 , yielding several nonpteridinic products. The quantum yields for PT disappearance were found to be 8.2 (-0.6)-10-4 and 1.2 (-0.2)-10-3 in acidic and alkaline media, respectively. H2O2 was detected and quantified in irradiated solutions of PT; and its importance from a biomedical point of view is discussed. The rate constant of the chemical reaction between singlet oxygen (1O2) and PT was determined to be 2.5 (-0.2)-105 l mol-1 s-1 in alkaline medium, and the role of 1O2 in the photooxidation of pterin was evaluated. © 2004 Verlag Helvetica Chimica Acta AG, Zürich.
format	JOUR
author	Cabrerizo, F.M. Dantola, M.L. Thomas, A.H. Lorente, C. Braun, A.M. Oliveros, E. Capparelli, A.L.
author_facet	Cabrerizo, F.M. Dantola, M.L. Thomas, A.H. Lorente, C. Braun, A.M. Oliveros, E. Capparelli, A.L.
author_sort	Cabrerizo, F.M.
title	Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
title_short	Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
title_full	Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
title_fullStr	Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
title_full_unstemmed	Photooxidation of pterin in aqueous solutions: Biological and biomedical implications
title_sort	photooxidation of pterin in aqueous solutions: biological and biomedical implications
url	http://hdl.handle.net/20.500.12110/paper_16121872_v1_n11_p1800_Cabrerizo
work_keys_str_mv	AT cabrerizofm photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT dantolaml photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT thomasah photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT lorentec photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT braunam photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT oliverose photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications AT capparellial photooxidationofpterininaqueoussolutionsbiologicalandbiomedicalimplications
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Photooxidation of pterin in aqueous solutions: Biological and biomedical implications

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