Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells
Quantum dots (QDs) are unique probes due to their special properties (brightness, photostability, narrowband emission and broadband absorption), and excellent bio(chemical)compatibility for imaging structures and functions of living cells. When functionalized with ligands, they enable the recognitio...
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todo:paper_16057422_v7189_n_p_Menendez2023-10-03T16:27:50Z Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells Menéndez, G. Roberti, M.J. Sigot, V. Etchehon, M. Jovin, T.M. Erijman, J.E.A. α-synuclein Amyloid protein aggregation Confocal microscopy. Imaging Nanoeffectors Parkinson's disease pH nanosensors Amyloid protein aggregation Amyloid proteins Broadband absorption Dynamic process Functionalized Imaging Imaging structure In-vitro Live cell Living cell Multivalency Nano-devices Nanoactuators Nanoeffectors Narrow-band emission Parkinson's disease Photo-stability Quantum Dot Quantum dots Sensing characteristics Single molecule level Small molecules Special properties Synuclein Cell membranes Confocal microscopy Energy transfer Glycoproteins Molecules Nanoparticles Nanosensors Optical properties Probes Semiconductor quantum dots Agglomeration Quantum dots (QDs) are unique probes due to their special properties (brightness, photostability, narrowband emission and broadband absorption), and excellent bio(chemical)compatibility for imaging structures and functions of living cells. When functionalized with ligands, they enable the recognition of specific targets and the tracking of dynamic processes for extended periods of time, detecting biomolecules with a sensitivity extending to the single molecule level. Thus, devices and probes based on such nanoparticles are very powerful tools for studying essential processes underlying the functions and regulation of living cells. Here we present nanosensors and nanoactuators based on QDs in which the multivalency of these particles plays an essential role in the functionality and sensing characteristics of the nanodevices. Two examples are discussed, the first being pH nanosensors based on the interplay of the multivalency and energy transfer between the nanoparticles and small molecules on their surface, and the second nanoactuators in which a controlled number of molecules of the amyloid protein α-synuclein (AS) specifically regulate the aggregation of fluorescently labeled bulk AS protein both in vitro and in live cells.©; 2009 SPIE. Fil:Menéndez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. CONF info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16057422_v7189_n_p_Menendez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
α-synuclein Amyloid protein aggregation Confocal microscopy. Imaging Nanoeffectors Parkinson's disease pH nanosensors Amyloid protein aggregation Amyloid proteins Broadband absorption Dynamic process Functionalized Imaging Imaging structure In-vitro Live cell Living cell Multivalency Nano-devices Nanoactuators Nanoeffectors Narrow-band emission Parkinson's disease Photo-stability Quantum Dot Quantum dots Sensing characteristics Single molecule level Small molecules Special properties Synuclein Cell membranes Confocal microscopy Energy transfer Glycoproteins Molecules Nanoparticles Nanosensors Optical properties Probes Semiconductor quantum dots Agglomeration |
| spellingShingle |
α-synuclein Amyloid protein aggregation Confocal microscopy. Imaging Nanoeffectors Parkinson's disease pH nanosensors Amyloid protein aggregation Amyloid proteins Broadband absorption Dynamic process Functionalized Imaging Imaging structure In-vitro Live cell Living cell Multivalency Nano-devices Nanoactuators Nanoeffectors Narrow-band emission Parkinson's disease Photo-stability Quantum Dot Quantum dots Sensing characteristics Single molecule level Small molecules Special properties Synuclein Cell membranes Confocal microscopy Energy transfer Glycoproteins Molecules Nanoparticles Nanosensors Optical properties Probes Semiconductor quantum dots Agglomeration Menéndez, G. Roberti, M.J. Sigot, V. Etchehon, M. Jovin, T.M. Erijman, J.E.A. Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| topic_facet |
α-synuclein Amyloid protein aggregation Confocal microscopy. Imaging Nanoeffectors Parkinson's disease pH nanosensors Amyloid protein aggregation Amyloid proteins Broadband absorption Dynamic process Functionalized Imaging Imaging structure In-vitro Live cell Living cell Multivalency Nano-devices Nanoactuators Nanoeffectors Narrow-band emission Parkinson's disease Photo-stability Quantum Dot Quantum dots Sensing characteristics Single molecule level Small molecules Special properties Synuclein Cell membranes Confocal microscopy Energy transfer Glycoproteins Molecules Nanoparticles Nanosensors Optical properties Probes Semiconductor quantum dots Agglomeration |
| description |
Quantum dots (QDs) are unique probes due to their special properties (brightness, photostability, narrowband emission and broadband absorption), and excellent bio(chemical)compatibility for imaging structures and functions of living cells. When functionalized with ligands, they enable the recognition of specific targets and the tracking of dynamic processes for extended periods of time, detecting biomolecules with a sensitivity extending to the single molecule level. Thus, devices and probes based on such nanoparticles are very powerful tools for studying essential processes underlying the functions and regulation of living cells. Here we present nanosensors and nanoactuators based on QDs in which the multivalency of these particles plays an essential role in the functionality and sensing characteristics of the nanodevices. Two examples are discussed, the first being pH nanosensors based on the interplay of the multivalency and energy transfer between the nanoparticles and small molecules on their surface, and the second nanoactuators in which a controlled number of molecules of the amyloid protein α-synuclein (AS) specifically regulate the aggregation of fluorescently labeled bulk AS protein both in vitro and in live cells.©; 2009 SPIE. |
| format |
CONF |
| author |
Menéndez, G. Roberti, M.J. Sigot, V. Etchehon, M. Jovin, T.M. Erijman, J.E.A. |
| author_facet |
Menéndez, G. Roberti, M.J. Sigot, V. Etchehon, M. Jovin, T.M. Erijman, J.E.A. |
| author_sort |
Menéndez, G. |
| title |
Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| title_short |
Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| title_full |
Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| title_fullStr |
Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| title_full_unstemmed |
Interplay of multivalency and optical properties of quantum dots: Implications for sensing and actuation in living cells |
| title_sort |
interplay of multivalency and optical properties of quantum dots: implications for sensing and actuation in living cells |
| url |
http://hdl.handle.net/20.500.12110/paper_16057422_v7189_n_p_Menendez |
| work_keys_str_mv |
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