Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae

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Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene...

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Autores principales:	Tranchida, M.C., Riccillo, P.M., Micieli, M.V., García, J.J., Rodriguero, M.S.
Formato:	JOUR
Materias:	16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Ochlerotatus albifasciatus Posibacteria
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15904261_v61_n3_p575_Tranchida
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spelling	todo:paper_15904261_v61_n3_p575_Tranchida2023-10-03T16:27:39Z Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae Tranchida, M.C. Riccillo, P.M. Micieli, M.V. García, J.J. Rodriguero, M.S. 16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria
spellingShingle	16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria Tranchida, M.C. Riccillo, P.M. Micieli, M.V. García, J.J. Rodriguero, M.S. Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
topic_facet	16S rRNA gene Biological control Culex pipiens Lysinibacillus sphaericus Mosquito larvae Aedes aegypti Anopheles albitarsis Bacillus licheniformis Bacteria (microorganisms) Culex pipiens Ochlerotatus albifasciatus Posibacteria
description	Three strains-C107, C207, and C307-of sporeforming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of Lysinibacillus sphaericus, while C307 was related to Bacillus licheniformis. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the binA, binB, and mtx genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: Culex pipiens, Aedes aegypti, Culex dolosus, Culex apicinus, Ochlerotatus albifasciatus, and Anopheles albitarsis. Only isolates C107 and C207 exhibited mosquitocidal activity. Culex pipiens was the species most susceptible to C107 (LC50, 4× 104 spores/ml), while O. albifasciatus was most susceptible to C207 (LC 50, 3.4× 106 spores/ml). © Springer-Verlag and the University of Milan 2010.
format	JOUR
author	Tranchida, M.C. Riccillo, P.M. Micieli, M.V. García, J.J. Rodriguero, M.S.
author_facet	Tranchida, M.C. Riccillo, P.M. Micieli, M.V. García, J.J. Rodriguero, M.S.
author_sort	Tranchida, M.C.
title	Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_short	Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_full	Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_fullStr	Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_full_unstemmed	Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae
title_sort	isolation, characterization and evaluation of mosquitocidal activity of lysinibacillus strains obtained from culex pipiens larvae
url	http://hdl.handle.net/20.500.12110/paper_15904261_v61_n3_p575_Tranchida
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_version_	1807322360853823488

Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae

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