The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer
Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly...
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todo:paper_15726657_v646_n1-2_p24_Flexer2023-10-03T16:27:31Z The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer Flexer, V. Calvo, E.J. Bartlett, P.N. Enzyme electrode Glucose oxidase Kinetics Layer-by-layer assembly Osmium redox polymer Electrodes Enzymes Film preparation Glucose Glucose sensors Gold coatings Multilayer films Optimization Osmium Polymers Rate constants Analysis of data Analytical expressions Digital simulation Enzyme electrode Enzyme loading Enzyme molecules Fully integrated Glucose concentration Gold electrodes Kinetic data Layer by layer self assembly Layer-by-layer assemblies Layer-by-layer assembly Polyelectrolyte layers Re-oxidation Redox hydrogels Redox polymers Reproducibilities Second orders Simplex methods Thickness dependence Glucose oxidase Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of "wired enzyme molecules" and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel. © 2010 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15726657_v646_n1-2_p24_Flexer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Enzyme electrode Glucose oxidase Kinetics Layer-by-layer assembly Osmium redox polymer Electrodes Enzymes Film preparation Glucose Glucose sensors Gold coatings Multilayer films Optimization Osmium Polymers Rate constants Analysis of data Analytical expressions Digital simulation Enzyme electrode Enzyme loading Enzyme molecules Fully integrated Glucose concentration Gold electrodes Kinetic data Layer by layer self assembly Layer-by-layer assemblies Layer-by-layer assembly Polyelectrolyte layers Re-oxidation Redox hydrogels Redox polymers Reproducibilities Second orders Simplex methods Thickness dependence Glucose oxidase |
| spellingShingle |
Enzyme electrode Glucose oxidase Kinetics Layer-by-layer assembly Osmium redox polymer Electrodes Enzymes Film preparation Glucose Glucose sensors Gold coatings Multilayer films Optimization Osmium Polymers Rate constants Analysis of data Analytical expressions Digital simulation Enzyme electrode Enzyme loading Enzyme molecules Fully integrated Glucose concentration Gold electrodes Kinetic data Layer by layer self assembly Layer-by-layer assemblies Layer-by-layer assembly Polyelectrolyte layers Re-oxidation Redox hydrogels Redox polymers Reproducibilities Second orders Simplex methods Thickness dependence Glucose oxidase Flexer, V. Calvo, E.J. Bartlett, P.N. The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| topic_facet |
Enzyme electrode Glucose oxidase Kinetics Layer-by-layer assembly Osmium redox polymer Electrodes Enzymes Film preparation Glucose Glucose sensors Gold coatings Multilayer films Optimization Osmium Polymers Rate constants Analysis of data Analytical expressions Digital simulation Enzyme electrode Enzyme loading Enzyme molecules Fully integrated Glucose concentration Gold electrodes Kinetic data Layer by layer self assembly Layer-by-layer assemblies Layer-by-layer assembly Polyelectrolyte layers Re-oxidation Redox hydrogels Redox polymers Reproducibilities Second orders Simplex methods Thickness dependence Glucose oxidase |
| description |
Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of "wired enzyme molecules" and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel. © 2010 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Flexer, V. Calvo, E.J. Bartlett, P.N. |
| author_facet |
Flexer, V. Calvo, E.J. Bartlett, P.N. |
| author_sort |
Flexer, V. |
| title |
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| title_short |
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| title_full |
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| title_fullStr |
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| title_full_unstemmed |
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| title_sort |
application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer |
| url |
http://hdl.handle.net/20.500.12110/paper_15726657_v646_n1-2_p24_Flexer |
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| _version_ |
1782028633651544064 |