Multiplexed imaging of intracellular protein networks
Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15524922_v89_n8_p761_Grecco |
| Aporte de: |
| id |
todo:paper_15524922_v89_n8_p761_Grecco |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_15524922_v89_n8_p761_Grecco2023-10-03T16:25:22Z Multiplexed imaging of intracellular protein networks Grecco, H.E. Imtiaz, S. Zamir, E. cell-to-cell variability cyclic immunofluorescence fluorescent proteins high-throughput microscopy immunofluorescence live cell imaging multicolor imaging multispectral imaging spatial organization spectral unmixing green fluorescent protein chemistry cytoplasm fluorescence microscopy genetics molecular imaging procedures protein protein interaction Cytoplasm Green Fluorescent Proteins Microscopy, Fluorescence Molecular Imaging Protein Interaction Maps Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed. © 2016 International Society for Advancement of Cytometry. © 2016 International Society for Advancement of Cytometry Fil:Grecco, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15524922_v89_n8_p761_Grecco |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cell-to-cell variability cyclic immunofluorescence fluorescent proteins high-throughput microscopy immunofluorescence live cell imaging multicolor imaging multispectral imaging spatial organization spectral unmixing green fluorescent protein chemistry cytoplasm fluorescence microscopy genetics molecular imaging procedures protein protein interaction Cytoplasm Green Fluorescent Proteins Microscopy, Fluorescence Molecular Imaging Protein Interaction Maps |
| spellingShingle |
cell-to-cell variability cyclic immunofluorescence fluorescent proteins high-throughput microscopy immunofluorescence live cell imaging multicolor imaging multispectral imaging spatial organization spectral unmixing green fluorescent protein chemistry cytoplasm fluorescence microscopy genetics molecular imaging procedures protein protein interaction Cytoplasm Green Fluorescent Proteins Microscopy, Fluorescence Molecular Imaging Protein Interaction Maps Grecco, H.E. Imtiaz, S. Zamir, E. Multiplexed imaging of intracellular protein networks |
| topic_facet |
cell-to-cell variability cyclic immunofluorescence fluorescent proteins high-throughput microscopy immunofluorescence live cell imaging multicolor imaging multispectral imaging spatial organization spectral unmixing green fluorescent protein chemistry cytoplasm fluorescence microscopy genetics molecular imaging procedures protein protein interaction Cytoplasm Green Fluorescent Proteins Microscopy, Fluorescence Molecular Imaging Protein Interaction Maps |
| description |
Cellular functions emerge from the collective action of a large number of different proteins. Understanding how these protein networks operate requires monitoring their components in intact cells. Due to intercellular and intracellular molecular variability, it is important to monitor simultaneously multiple components at high spatiotemporal resolution. However, inherent trade-offs narrow the boundaries of achievable multiplexed imaging. Pushing these boundaries is essential for a better understanding of cellular processes. Here the motivations, challenges and approaches for multiplexed imaging of intracellular protein networks are discussed. © 2016 International Society for Advancement of Cytometry. © 2016 International Society for Advancement of Cytometry |
| format |
JOUR |
| author |
Grecco, H.E. Imtiaz, S. Zamir, E. |
| author_facet |
Grecco, H.E. Imtiaz, S. Zamir, E. |
| author_sort |
Grecco, H.E. |
| title |
Multiplexed imaging of intracellular protein networks |
| title_short |
Multiplexed imaging of intracellular protein networks |
| title_full |
Multiplexed imaging of intracellular protein networks |
| title_fullStr |
Multiplexed imaging of intracellular protein networks |
| title_full_unstemmed |
Multiplexed imaging of intracellular protein networks |
| title_sort |
multiplexed imaging of intracellular protein networks |
| url |
http://hdl.handle.net/20.500.12110/paper_15524922_v89_n8_p761_Grecco |
| work_keys_str_mv |
AT greccohe multiplexedimagingofintracellularproteinnetworks AT imtiazs multiplexedimagingofintracellularproteinnetworks AT zamire multiplexedimagingofintracellularproteinnetworks |
| _version_ |
1807321943612850176 |