Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase
Polyisoprenyl-pyrophosphate-linked cellobiose is the natural acceptor of the α-1,3-mannosyltransferase AceA from Acetobacter xylinum, which transfers mannose from GDPmannose during the assembly of the heptasaccharide repeat unit of the exopolysaccharide acetan. Phytanyl α-D- cellobiosyldiphosphate 4...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_1551-701_v2011_n7_p38_Barrios |
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todo:paper_1551-701_v2011_n7_p38_Barrios2023-10-03T16:25:20Z Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase Barrios, P. Ielpi, L. Marino, C. Acetobacter xylinium Glycosyl phosphate Mannosyltransferase Phytanyl phosphate Polyisoprenyl-pyrophosphate-linked cellobiose is the natural acceptor of the α-1,3-mannosyltransferase AceA from Acetobacter xylinum, which transfers mannose from GDPmannose during the assembly of the heptasaccharide repeat unit of the exopolysaccharide acetan. Phytanyl α-D- cellobiosyldiphosphate 4 has been previously synthesized as an analogue acceptor by condensation of hepta-O-acetyl-α-D-cellobiosylphosphate 1 with phytanylphosphate 2, but the procedure was briefly described. We report here a modified detailed synthesis of 4. The complete NMR characterization of 4 is provided and also a selection of NMR signals of all the intermediate compounds which facilitate monitoring the synthesis. © ARKAT-USA, Inc. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marino, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1551-701_v2011_n7_p38_Barrios |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acetobacter xylinium Glycosyl phosphate Mannosyltransferase Phytanyl phosphate |
| spellingShingle |
Acetobacter xylinium Glycosyl phosphate Mannosyltransferase Phytanyl phosphate Barrios, P. Ielpi, L. Marino, C. Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| topic_facet |
Acetobacter xylinium Glycosyl phosphate Mannosyltransferase Phytanyl phosphate |
| description |
Polyisoprenyl-pyrophosphate-linked cellobiose is the natural acceptor of the α-1,3-mannosyltransferase AceA from Acetobacter xylinum, which transfers mannose from GDPmannose during the assembly of the heptasaccharide repeat unit of the exopolysaccharide acetan. Phytanyl α-D- cellobiosyldiphosphate 4 has been previously synthesized as an analogue acceptor by condensation of hepta-O-acetyl-α-D-cellobiosylphosphate 1 with phytanylphosphate 2, but the procedure was briefly described. We report here a modified detailed synthesis of 4. The complete NMR characterization of 4 is provided and also a selection of NMR signals of all the intermediate compounds which facilitate monitoring the synthesis. © ARKAT-USA, Inc. |
| format |
JOUR |
| author |
Barrios, P. Ielpi, L. Marino, C. |
| author_facet |
Barrios, P. Ielpi, L. Marino, C. |
| author_sort |
Barrios, P. |
| title |
Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| title_short |
Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| title_full |
Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| title_fullStr |
Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| title_full_unstemmed |
Improved synthesis of phytanyl α-D-cellobiosyldiphosphate as substrate for α-D-mannosyltransferase |
| title_sort |
improved synthesis of phytanyl α-d-cellobiosyldiphosphate as substrate for α-d-mannosyltransferase |
| url |
http://hdl.handle.net/20.500.12110/paper_1551-701_v2011_n7_p38_Barrios |
| work_keys_str_mv |
AT barriosp improvedsynthesisofphytanyladcellobiosyldiphosphateassubstrateforadmannosyltransferase AT ielpil improvedsynthesisofphytanyladcellobiosyldiphosphateassubstrateforadmannosyltransferase AT marinoc improvedsynthesisofphytanyladcellobiosyldiphosphateassubstrateforadmannosyltransferase |
| _version_ |
1782026514483642368 |