Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT

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Serine/arginine-rich (SR) proteins are important regulators of mRNA splicing. Several postsplicing activities have been described for a subset of shuttling SR proteins, including regulation of mRNA export and translation. Using the fibronectin gene to study the links between signal-transduction path...

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Autores principales: Blaustein, M., Pelisch, F., Tanos, T., Muñoz, M.J., Wengier, D., Quadrana, L., Sanford, J.R., Muschietti, J.P., Kornblihtt, A.R., Cáceres, J.F., Coso, O.A., Srebrow, A.
Formato: JOUR
Materias:
fibronectin
growth factor
phosphatidylinositol 3 kinase
protein
protein kinase B
serine arginine rich protein
transfer RNA
unclassified drug
alternative RNA splicing
article
cytoplasm
human
nonhuman
priority journal
protein expression
protein function
protein localization
RNA translation
signal transduction
1-Phosphatidylinositol 3-Kinase
Amino Acid Sequence
Animals
Cell Nucleus
Cytoplasm
Fibronectins
Growth Substances
Humans
Molecular Sequence Data
Nuclear Proteins
Phosphoproteins
Protein Biosynthesis
Proto-Oncogene Proteins c-akt
RNA Splicing
RNA, Messenger
RNA, Small Interfering
RNA-Binding Proteins
Signal Transduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_15459993_v12_n12_p1037_Blaustein
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_15459993_v12_n12_p1037_Blaustein
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spelling todo:paper_15459993_v12_n12_p1037_Blaustein2023-10-03T16:23:04Z Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT Blaustein, M. Pelisch, F. Tanos, T. Muñoz, M.J. Wengier, D. Quadrana, L. Sanford, J.R. Muschietti, J.P. Kornblihtt, A.R. Cáceres, J.F. Coso, O.A. Srebrow, A. fibronectin growth factor phosphatidylinositol 3 kinase protein protein kinase B serine arginine rich protein transfer RNA unclassified drug alternative RNA splicing article cytoplasm human nonhuman priority journal protein expression protein function protein localization RNA translation signal transduction 1-Phosphatidylinositol 3-Kinase Amino Acid Sequence Animals Cell Nucleus Cytoplasm Fibronectins Growth Substances Humans Molecular Sequence Data Nuclear Proteins Phosphoproteins Protein Biosynthesis Proto-Oncogene Proteins c-akt RNA Splicing RNA, Messenger RNA, Small Interfering RNA-Binding Proteins Signal Transduction Serine/arginine-rich (SR) proteins are important regulators of mRNA splicing. Several postsplicing activities have been described for a subset of shuttling SR proteins, including regulation of mRNA export and translation. Using the fibronectin gene to study the links between signal-transduction pathways and SR protein activity, we show that growth factors not only modify the alternative splicing pattern of the fibronectin gene but also alter translation of reporter messenger RNAs in an SR protein-dependent fashion, providing two coregulated levels of isoform-specific amplification. These effects are inhibited by specific small interfering RNAs against SR proteins and are mediated by the AKT kinase, which elicits opposite effects to those evoked by overexpressing SR protein kinases CIk and SRPK. These results show how SR protein activity is modified in response to extracellular stimulation, leading to a concerted regulation of splicing and translation. © 2005 Nature Publishing Group. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pelisch, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tanos, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wengier, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quadrana, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cáceres, J.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15459993_v12_n12_p1037_Blaustein
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic fibronectin
growth factor
phosphatidylinositol 3 kinase
protein
protein kinase B
serine arginine rich protein
transfer RNA
unclassified drug
alternative RNA splicing
article
cytoplasm
human
nonhuman
priority journal
protein expression
protein function
protein localization
RNA translation
signal transduction
1-Phosphatidylinositol 3-Kinase
Amino Acid Sequence
Animals
Cell Nucleus
Cytoplasm
Fibronectins
Growth Substances
Humans
Molecular Sequence Data
Nuclear Proteins
Phosphoproteins
Protein Biosynthesis
Proto-Oncogene Proteins c-akt
RNA Splicing
RNA, Messenger
RNA, Small Interfering
RNA-Binding Proteins
Signal Transduction
spellingShingle fibronectin
growth factor
phosphatidylinositol 3 kinase
protein
protein kinase B
serine arginine rich protein
transfer RNA
unclassified drug
alternative RNA splicing
article
cytoplasm
human
nonhuman
priority journal
protein expression
protein function
protein localization
RNA translation
signal transduction
1-Phosphatidylinositol 3-Kinase
Amino Acid Sequence
Animals
Cell Nucleus
Cytoplasm
Fibronectins
Growth Substances
Humans
Molecular Sequence Data
Nuclear Proteins
Phosphoproteins
Protein Biosynthesis
Proto-Oncogene Proteins c-akt
RNA Splicing
RNA, Messenger
RNA, Small Interfering
RNA-Binding Proteins
Signal Transduction
Blaustein, M.
Pelisch, F.
Tanos, T.
Muñoz, M.J.
Wengier, D.
Quadrana, L.
Sanford, J.R.
Muschietti, J.P.
Kornblihtt, A.R.
Cáceres, J.F.
Coso, O.A.
Srebrow, A.
Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
topic_facet fibronectin
growth factor
phosphatidylinositol 3 kinase
protein
protein kinase B
serine arginine rich protein
transfer RNA
unclassified drug
alternative RNA splicing
article
cytoplasm
human
nonhuman
priority journal
protein expression
protein function
protein localization
RNA translation
signal transduction
1-Phosphatidylinositol 3-Kinase
Amino Acid Sequence
Animals
Cell Nucleus
Cytoplasm
Fibronectins
Growth Substances
Humans
Molecular Sequence Data
Nuclear Proteins
Phosphoproteins
Protein Biosynthesis
Proto-Oncogene Proteins c-akt
RNA Splicing
RNA, Messenger
RNA, Small Interfering
RNA-Binding Proteins
Signal Transduction
description Serine/arginine-rich (SR) proteins are important regulators of mRNA splicing. Several postsplicing activities have been described for a subset of shuttling SR proteins, including regulation of mRNA export and translation. Using the fibronectin gene to study the links between signal-transduction pathways and SR protein activity, we show that growth factors not only modify the alternative splicing pattern of the fibronectin gene but also alter translation of reporter messenger RNAs in an SR protein-dependent fashion, providing two coregulated levels of isoform-specific amplification. These effects are inhibited by specific small interfering RNAs against SR proteins and are mediated by the AKT kinase, which elicits opposite effects to those evoked by overexpressing SR protein kinases CIk and SRPK. These results show how SR protein activity is modified in response to extracellular stimulation, leading to a concerted regulation of splicing and translation. © 2005 Nature Publishing Group.
format JOUR
author Blaustein, M.
Pelisch, F.
Tanos, T.
Muñoz, M.J.
Wengier, D.
Quadrana, L.
Sanford, J.R.
Muschietti, J.P.
Kornblihtt, A.R.
Cáceres, J.F.
Coso, O.A.
Srebrow, A.
author_facet Blaustein, M.
Pelisch, F.
Tanos, T.
Muñoz, M.J.
Wengier, D.
Quadrana, L.
Sanford, J.R.
Muschietti, J.P.
Kornblihtt, A.R.
Cáceres, J.F.
Coso, O.A.
Srebrow, A.
author_sort Blaustein, M.
title Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
title_short Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
title_full Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
title_fullStr Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
title_full_unstemmed Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT
title_sort concerted regulation of nuclear and cytoplasmic activities of sr proteins by akt
url http://hdl.handle.net/20.500.12110/paper_15459993_v12_n12_p1037_Blaustein
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