Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood

Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative...

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Autores principales:	Poletta, G.L., Simoniello, M.F., Mudry, M.D.
Formato:	JOUR
Materias:	Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant DNA formamidopyrimidine glycosylase animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism physiology Alligators and Crocodiles Animals Antioxidants DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15320456_v179_n_p29_Poletta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_15320456_v179_n_p29_Poletta2023-10-03T16:21:29Z Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood Poletta, G.L. Simoniello, M.F. Mudry, M.D. Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by thiobarbituric acid reactive substances (TBARS), 2) damage to DNA by comet assay modified with the enzymes FPG and Endo III, and 3) antioxidant defenses: catalase, superoxide dismutase and glutathione; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for the detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities. © 2015 Elsevier Inc. Fil:Poletta, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15320456_v179_n_p29_Poletta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances
spellingShingle	Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances Poletta, G.L. Simoniello, M.F. Mudry, M.D. Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
topic_facet	Biomarkers Crocodiles Oxidative stress Reptiles Xenobiotics biological marker catalase DNA glutathione lipid superoxide dismutase thiobarbituric acid reactive substance xenobiotic agent antioxidant biological marker DNA formamidopyrimidine glycosylase thiobarbituric acid reactive substance animal cell antioxidant assay Article biological monitoring blood analysis caiman Caiman latirostris calibration comet assay controlled study dilution DNA damage enzyme assay erythrocyte female lipid peroxidation male nonhuman oxidative stress priority journal animal blood crocodilian dose response metabolism oxidative stress physiology Alligators and Crocodiles Animals Antioxidants Biomarkers DNA Damage DNA-Formamidopyrimidine Glycosylase Dose-Response Relationship, Drug Erythrocytes Female Male Oxidative Stress Thiobarbituric Acid Reactive Substances
description	Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by thiobarbituric acid reactive substances (TBARS), 2) damage to DNA by comet assay modified with the enzymes FPG and Endo III, and 3) antioxidant defenses: catalase, superoxide dismutase and glutathione; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for the detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities. © 2015 Elsevier Inc.
format	JOUR
author	Poletta, G.L. Simoniello, M.F. Mudry, M.D.
author_facet	Poletta, G.L. Simoniello, M.F. Mudry, M.D.
author_sort	Poletta, G.L.
title	Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
title_short	Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
title_full	Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
title_fullStr	Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
title_full_unstemmed	Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood
title_sort	biomarkers of oxidative damage and antioxidant defense capacity in caiman latirostris blood
url	http://hdl.handle.net/20.500.12110/paper_15320456_v179_n_p29_Poletta
work_keys_str_mv	AT polettagl biomarkersofoxidativedamageandantioxidantdefensecapacityincaimanlatirostrisblood AT simoniellomf biomarkersofoxidativedamageandantioxidantdefensecapacityincaimanlatirostrisblood AT mudrymd biomarkersofoxidativedamageandantioxidantdefensecapacityincaimanlatirostrisblood
_version_	1807316700269379584

Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood

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