Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids

The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decre...

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Autores principales:	Pellegrotti, J.V., Acuna, G.P., Puchkova, A., Holzmeister, P., Gietl, A., Lalkens, B., Stefani, F.D., Tinnefeld, P.
Formato:	JOUR
Materias:	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques Metal Nanoparticles Photons
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15306984_v14_n5_p2831_Pellegrotti
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spelling	todo:paper_15306984_v14_n5_p2831_Pellegrotti2023-10-03T16:21:19Z Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids Pellegrotti, J.V. Acuna, G.P. Puchkova, A. Holzmeister, P. Gietl, A. Lalkens, B. Stefani, F.D. Tinnefeld, P. DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. © 2014 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons
spellingShingle	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons Pellegrotti, J.V. Acuna, G.P. Puchkova, A. Holzmeister, P. Gietl, A. Lalkens, B. Stefani, F.D. Tinnefeld, P. Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
topic_facet	DNA self-assembly gold nanoparticle photobleaching plasmonics single-molecule DNA Emission spectroscopy Fluorescence Fluorophores Gold Metal nanoparticles Molecules Self assembly DNA self-assembly Fluorescence intensities Gold Nanoparticles Plasmonics Single molecule measurements Single-molecule Single-molecule studies Single-molecule tracking Photobleaching DNA gold metal nanoparticle bleaching chemistry genetic procedures photon Biosensing Techniques DNA Gold Metal Nanoparticles Photobleaching Photons
description	The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. © 2014 American Chemical Society.
format	JOUR
author	Pellegrotti, J.V. Acuna, G.P. Puchkova, A. Holzmeister, P. Gietl, A. Lalkens, B. Stefani, F.D. Tinnefeld, P.
author_facet	Pellegrotti, J.V. Acuna, G.P. Puchkova, A. Holzmeister, P. Gietl, A. Lalkens, B. Stefani, F.D. Tinnefeld, P.
author_sort	Pellegrotti, J.V.
title	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_short	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_full	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_fullStr	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_full_unstemmed	Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids
title_sort	controlled reduction of photobleaching in dna origami-gold nanoparticle hybrids
url	http://hdl.handle.net/20.500.12110/paper_15306984_v14_n5_p2831_Pellegrotti
work_keys_str_mv	AT pellegrottijv controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT acunagp controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT puchkovaa controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT holzmeisterp controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT gietla controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT lalkensb controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT stefanifd controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids AT tinnefeldp controlledreductionofphotobleachingindnaorigamigoldnanoparticlehybrids
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Controlled reduction of photobleaching in DNA origami-gold nanoparticle hybrids

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