Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations
Neuroglobin (Ngb) is a heme protein, highly conserved along evolution, predominantly found in the nervous system. It is upregulated by hypoxia and ischemia and may have a neuroprotective role under hypoxic stress. Although many other roles have been proposed, the physiological function is still uncl...
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todo:paper_15216543_v63_n3_p206_Boron2023-10-03T16:20:38Z Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations Boron, I. Russo, R. Boechi, L. Cheng, C.-H.C. Di Prisco, G. Estrin, D.A. Verde, C. Nadra, A.D. evolution hemeproteins neuroglobin protein function protein structure structural biology neuroglobin amino terminal sequence Antarctica carboxy terminal sequence computer simulation conference paper fish gene sequence molecular dynamics mutation nonhuman nucleotide sequence protein analysis protein function protein structure Amino Acid Sequence Animals Antarctic Regions Base Sequence Computer Simulation DNA Primers Fishes Globins Molecular Dynamics Simulation Molecular Sequence Data Nerve Tissue Proteins Sequence Homology, Amino Acid Chaenocephalus aceratus Dissostichus mawsoni Mammalia Salangini Neuroglobin (Ngb) is a heme protein, highly conserved along evolution, predominantly found in the nervous system. It is upregulated by hypoxia and ischemia and may have a neuroprotective role under hypoxic stress. Although many other roles have been proposed, the physiological function is still unclear. Antarctic icefishes lack hemoglobin and some species also lack myoglobin, but all have Ngb and thus may help the elucidation of Ngb function. We present the first theoretically derived structure of fish Ngb and describe its behavior using molecular dynamics simulations. Specifically, we sequenced and analyzed Ngbs from a colorless-blooded Antarctic icefish species Chaenocephalus aceratus and a related red-blooded species (Dissostichus mawsoni). Both fish Ngbs are 6-coordinated but have some peculiarities that differentiate them from mammalian counterparts: they have extensions in the N and C termini that can interact with the EF loop, and a gap in the alignment that changes the CD-region structure/dynamics that has been found to play a key role in human neuroglobin. Our results suggest that a single mutation between both fish Ngbs is responsible for significant difference in the behavior of the proteins. The functional role of these characteristics is discussed. © 2011 IUBMB. Fil:Boechi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estrin, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nadra, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15216543_v63_n3_p206_Boron |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
evolution hemeproteins neuroglobin protein function protein structure structural biology neuroglobin amino terminal sequence Antarctica carboxy terminal sequence computer simulation conference paper fish gene sequence molecular dynamics mutation nonhuman nucleotide sequence protein analysis protein function protein structure Amino Acid Sequence Animals Antarctic Regions Base Sequence Computer Simulation DNA Primers Fishes Globins Molecular Dynamics Simulation Molecular Sequence Data Nerve Tissue Proteins Sequence Homology, Amino Acid Chaenocephalus aceratus Dissostichus mawsoni Mammalia Salangini |
| spellingShingle |
evolution hemeproteins neuroglobin protein function protein structure structural biology neuroglobin amino terminal sequence Antarctica carboxy terminal sequence computer simulation conference paper fish gene sequence molecular dynamics mutation nonhuman nucleotide sequence protein analysis protein function protein structure Amino Acid Sequence Animals Antarctic Regions Base Sequence Computer Simulation DNA Primers Fishes Globins Molecular Dynamics Simulation Molecular Sequence Data Nerve Tissue Proteins Sequence Homology, Amino Acid Chaenocephalus aceratus Dissostichus mawsoni Mammalia Salangini Boron, I. Russo, R. Boechi, L. Cheng, C.-H.C. Di Prisco, G. Estrin, D.A. Verde, C. Nadra, A.D. Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| topic_facet |
evolution hemeproteins neuroglobin protein function protein structure structural biology neuroglobin amino terminal sequence Antarctica carboxy terminal sequence computer simulation conference paper fish gene sequence molecular dynamics mutation nonhuman nucleotide sequence protein analysis protein function protein structure Amino Acid Sequence Animals Antarctic Regions Base Sequence Computer Simulation DNA Primers Fishes Globins Molecular Dynamics Simulation Molecular Sequence Data Nerve Tissue Proteins Sequence Homology, Amino Acid Chaenocephalus aceratus Dissostichus mawsoni Mammalia Salangini |
| description |
Neuroglobin (Ngb) is a heme protein, highly conserved along evolution, predominantly found in the nervous system. It is upregulated by hypoxia and ischemia and may have a neuroprotective role under hypoxic stress. Although many other roles have been proposed, the physiological function is still unclear. Antarctic icefishes lack hemoglobin and some species also lack myoglobin, but all have Ngb and thus may help the elucidation of Ngb function. We present the first theoretically derived structure of fish Ngb and describe its behavior using molecular dynamics simulations. Specifically, we sequenced and analyzed Ngbs from a colorless-blooded Antarctic icefish species Chaenocephalus aceratus and a related red-blooded species (Dissostichus mawsoni). Both fish Ngbs are 6-coordinated but have some peculiarities that differentiate them from mammalian counterparts: they have extensions in the N and C termini that can interact with the EF loop, and a gap in the alignment that changes the CD-region structure/dynamics that has been found to play a key role in human neuroglobin. Our results suggest that a single mutation between both fish Ngbs is responsible for significant difference in the behavior of the proteins. The functional role of these characteristics is discussed. © 2011 IUBMB. |
| format |
JOUR |
| author |
Boron, I. Russo, R. Boechi, L. Cheng, C.-H.C. Di Prisco, G. Estrin, D.A. Verde, C. Nadra, A.D. |
| author_facet |
Boron, I. Russo, R. Boechi, L. Cheng, C.-H.C. Di Prisco, G. Estrin, D.A. Verde, C. Nadra, A.D. |
| author_sort |
Boron, I. |
| title |
Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| title_short |
Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| title_full |
Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| title_fullStr |
Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| title_full_unstemmed |
Structure and dynamics of Antarctic fish neuroglobin assessed by computer simulations |
| title_sort |
structure and dynamics of antarctic fish neuroglobin assessed by computer simulations |
| url |
http://hdl.handle.net/20.500.12110/paper_15216543_v63_n3_p206_Boron |
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