Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better

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The realization that the mammalian proteomic complexity is achieved with a limited number of genes demands a better understanding of alternative splicing regulation. Promoter control of alternative splicing was originally described by our group in studies performed on the fibronectin gene. Recently,...

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Autores principales:	Nogués, G., Kadener, S., Cramer, P., De la Mata, M., Fededa, J.P., Blaustein, M., Srebrow, A., Kornblihtt, A.R.
Formato:	JOUR
Materias:	Alternative splicing Elongation mRNA processing RNA polymerase II Transcription calcitonin gene related peptide fibronectin Hermes antigen messenger RNA protein proteome transcription factor alternative RNA splicing animal cell controlled study cystic fibrosis DNA template gene control gene function genetic code genetic transcription mammalian genetics nonhuman promoter region protein RNA binding proteomics regulatory mechanism review Alternative Splicing Animals Models, Genetic RNA Polymerase II RNA Precursors RNA, Messenger RNA-Binding Proteins Transcription, Genetic Animalia Mammalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15216543_v55_n4-5_p235_Nogues
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_15216543_v55_n4-5_p235_Nogues
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spelling	todo:paper_15216543_v55_n4-5_p235_Nogues2023-10-03T16:20:36Z Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better Nogués, G. Kadener, S. Cramer, P. De la Mata, M. Fededa, J.P. Blaustein, M. Srebrow, A. Kornblihtt, A.R. Alternative splicing Elongation mRNA processing RNA polymerase II Transcription calcitonin gene related peptide fibronectin Hermes antigen messenger RNA protein proteome transcription factor alternative RNA splicing animal cell controlled study cystic fibrosis DNA template gene control gene function genetic code genetic transcription mammalian genetics nonhuman promoter region protein RNA binding proteomics regulatory mechanism review Alternative Splicing Animals Models, Genetic RNA Polymerase II RNA Precursors RNA, Messenger RNA-Binding Proteins Transcription, Genetic Animalia Mammalia The realization that the mammalian proteomic complexity is achieved with a limited number of genes demands a better understanding of alternative splicing regulation. Promoter control of alternative splicing was originally described by our group in studies performed on the fibronectin gene. Recently, other labs extended our findings to the cystic fibrosis, CD44 and CGRP genes strongly supporting a coupling between transcription and pre-mRNA splicing. A possible mechanism that would fit in these results is that the promoter itself is responsible for recruiting splicing factors, such as SR proteins, to the site of transcription, possibly through transcription factors that bind the promoter or the transcriptional enhancers. An alternative model, discussed more extensively in this review, involves modulation of RNA pol II (pol II) elongation rate. The model is supported by findings that cis- and trans-acting factors that modulate pol II elongation on a particular template also provoke changes in the alternative splicing balance of the encoded mRNAs. Fil:Nogués, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kadener, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De la Mata, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fededa, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15216543_v55_n4-5_p235_Nogues
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alternative splicing Elongation mRNA processing RNA polymerase II Transcription calcitonin gene related peptide fibronectin Hermes antigen messenger RNA protein proteome transcription factor alternative RNA splicing animal cell controlled study cystic fibrosis DNA template gene control gene function genetic code genetic transcription mammalian genetics nonhuman promoter region protein RNA binding proteomics regulatory mechanism review Alternative Splicing Animals Models, Genetic RNA Polymerase II RNA Precursors RNA, Messenger RNA-Binding Proteins Transcription, Genetic Animalia Mammalia
spellingShingle	Alternative splicing Elongation mRNA processing RNA polymerase II Transcription calcitonin gene related peptide fibronectin Hermes antigen messenger RNA protein proteome transcription factor alternative RNA splicing animal cell controlled study cystic fibrosis DNA template gene control gene function genetic code genetic transcription mammalian genetics nonhuman promoter region protein RNA binding proteomics regulatory mechanism review Alternative Splicing Animals Models, Genetic RNA Polymerase II RNA Precursors RNA, Messenger RNA-Binding Proteins Transcription, Genetic Animalia Mammalia Nogués, G. Kadener, S. Cramer, P. De la Mata, M. Fededa, J.P. Blaustein, M. Srebrow, A. Kornblihtt, A.R. Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
topic_facet	Alternative splicing Elongation mRNA processing RNA polymerase II Transcription calcitonin gene related peptide fibronectin Hermes antigen messenger RNA protein proteome transcription factor alternative RNA splicing animal cell controlled study cystic fibrosis DNA template gene control gene function genetic code genetic transcription mammalian genetics nonhuman promoter region protein RNA binding proteomics regulatory mechanism review Alternative Splicing Animals Models, Genetic RNA Polymerase II RNA Precursors RNA, Messenger RNA-Binding Proteins Transcription, Genetic Animalia Mammalia
description	The realization that the mammalian proteomic complexity is achieved with a limited number of genes demands a better understanding of alternative splicing regulation. Promoter control of alternative splicing was originally described by our group in studies performed on the fibronectin gene. Recently, other labs extended our findings to the cystic fibrosis, CD44 and CGRP genes strongly supporting a coupling between transcription and pre-mRNA splicing. A possible mechanism that would fit in these results is that the promoter itself is responsible for recruiting splicing factors, such as SR proteins, to the site of transcription, possibly through transcription factors that bind the promoter or the transcriptional enhancers. An alternative model, discussed more extensively in this review, involves modulation of RNA pol II (pol II) elongation rate. The model is supported by findings that cis- and trans-acting factors that modulate pol II elongation on a particular template also provoke changes in the alternative splicing balance of the encoded mRNAs.
format	JOUR
author	Nogués, G. Kadener, S. Cramer, P. De la Mata, M. Fededa, J.P. Blaustein, M. Srebrow, A. Kornblihtt, A.R.
author_facet	Nogués, G. Kadener, S. Cramer, P. De la Mata, M. Fededa, J.P. Blaustein, M. Srebrow, A. Kornblihtt, A.R.
author_sort	Nogués, G.
title	Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
title_short	Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
title_full	Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
title_fullStr	Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
title_full_unstemmed	Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better
title_sort	control of alternative pre-mrna splicing by rna pol ii elongation: faster is not always better
url	http://hdl.handle.net/20.500.12110/paper_15216543_v55_n4-5_p235_Nogues
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Control of alternative pre-mRNA splicing by RNA pol II elongation: Faster is not always better

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