High glucose-induced changes in steroid production in adrenal cells

Background: Increased activity of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in enhanced adrenocorticotropin (ACTH) and serum glucocorticoid levels, has been described in patients with diabetes mellitus and in animal models of this disease; however, altered steroid production by adreno...

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Autores principales:	Astort, F., Repetto, E.M., Martínez Calejman, C., Cipelli, J.M., Sánchez, R., Di Gruccio, J.M., Mercau, M., Pignataro, O.P., Arias, P., Cymeryng, C.B.
Formato:	JOUR
Materias:	Adrenocortical cells Antioxidants Haeme oxygenase-1 High glucose Nitric oxide Oxidative stress Steroids arginine corticotropin glucose glutathione heme oxygenase 1 lipid peroxide nitrite progesterone reactive oxygen metabolite heme oxygenase thiobarbituric acid reactive substance adrenal cortex cell animal cell article controlled study enzyme activity enzyme inhibition gene silencing hormone response hyperglycemia mouse nonhuman nucleotide sequence oxidative stress priority journal progesterone synthesis protein expression rat steroidogenesis analysis of variance animal cell clone cell culture comparative study cytology dose response genetic transfection human metabolism nonparametric test physiology zona fasciculata Analysis of Variance Animals Arginine Cells, Cultured Clone Cells Dose-Response Relationship, Drug Glucose Heme Oxygenase (Decyclizing) Humans Hyperglycemia Mice Nitrites Oxidative Stress Progesterone Rats Reactive Oxygen Species Statistics, Nonparametric Thiobarbituric Acid Reactive Substances Transfection Zona Fasciculata
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_15207552_v25_n5_p477_Astort
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_15207552_v25_n5_p477_Astort2023-10-03T16:20:35Z High glucose-induced changes in steroid production in adrenal cells Astort, F. Repetto, E.M. Martínez Calejman, C. Cipelli, J.M. Sánchez, R. Di Gruccio, J.M. Mercau, M. Pignataro, O.P. Arias, P. Cymeryng, C.B. Adrenocortical cells Antioxidants Haeme oxygenase-1 High glucose Nitric oxide Oxidative stress Steroids arginine corticotropin glucose glutathione heme oxygenase 1 lipid peroxide nitrite progesterone reactive oxygen metabolite arginine glucose heme oxygenase nitrite progesterone reactive oxygen metabolite thiobarbituric acid reactive substance adrenal cortex cell animal cell article controlled study enzyme activity enzyme inhibition gene silencing hormone response hyperglycemia mouse nonhuman nucleotide sequence oxidative stress priority journal progesterone synthesis protein expression rat steroidogenesis analysis of variance animal cell clone cell culture comparative study cytology dose response genetic transfection human metabolism nonparametric test oxidative stress physiology zona fasciculata Analysis of Variance Animals Arginine Cells, Cultured Clone Cells Dose-Response Relationship, Drug Glucose Heme Oxygenase (Decyclizing) Humans Hyperglycemia Mice Nitrites Oxidative Stress Progesterone Rats Reactive Oxygen Species Statistics, Nonparametric Thiobarbituric Acid Reactive Substances Transfection Zona Fasciculata Background: Increased activity of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in enhanced adrenocorticotropin (ACTH) and serum glucocorticoid levels, has been described in patients with diabetes mellitus and in animal models of this disease; however, altered steroid production by adrenocortical cells could result from local changes triggered by increased reactive oxygen species (ROS), induced in turn by chronic hyperglycaemia. Experiments were designed (1) to analyse the effects of incubating murine adrenocortical cells in hyperglycaemic media on the generation of oxidative stress, on steroid synthesis and on its modulation by the activity of haeme oxygenase (HO); and (2) to evaluate the effect of antioxidant treatment on these parameters. Methods: Y1 cells were incubated for 7 days with either normal or high glucose (HG, 30 mmol/L) concentrations, with or without antioxidant treatment. Parameters of oxidative stress and expression levels of haeme oxygenase-1 (HO-1), nitrite levels, L-arginine uptake and progesterone production were determined. Results: HG augmented ROS and lipoperoxide production, decreasing glutathione (GSH) levels and increasing antioxidant enzymes and HO-1 expression. Basal progesterone production was reduced, while a higher response to ACTH was observed in HG-treated cells. The increase in HO-1 expression and the effects on basal steroid production were abolished by antioxidant treatment. Inhibition of HO activity increased basal and ACTH-stimulated steroid release. Similar results were obtained by HO-1 gene silencing while the opposite effect was observed in Y1 cells overexpressing HO-1. Conclusions?: HG induces oxidative stress and affects steroid production in adrenal cells; the involvement of HO activity in the modulation of steroidogenesis in Y1 cells is postulated. Copyright © 2009 John Wiley & Sons, Ltd. Fil:Astort, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez Calejman, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cymeryng, C.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15207552_v25_n5_p477_Astort
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adrenocortical cells Antioxidants Haeme oxygenase-1 High glucose Nitric oxide Oxidative stress Steroids arginine corticotropin glucose glutathione heme oxygenase 1 lipid peroxide nitrite progesterone reactive oxygen metabolite arginine glucose heme oxygenase nitrite progesterone reactive oxygen metabolite thiobarbituric acid reactive substance adrenal cortex cell animal cell article controlled study enzyme activity enzyme inhibition gene silencing hormone response hyperglycemia mouse nonhuman nucleotide sequence oxidative stress priority journal progesterone synthesis protein expression rat steroidogenesis analysis of variance animal cell clone cell culture comparative study cytology dose response genetic transfection human metabolism nonparametric test oxidative stress physiology zona fasciculata Analysis of Variance Animals Arginine Cells, Cultured Clone Cells Dose-Response Relationship, Drug Glucose Heme Oxygenase (Decyclizing) Humans Hyperglycemia Mice Nitrites Oxidative Stress Progesterone Rats Reactive Oxygen Species Statistics, Nonparametric Thiobarbituric Acid Reactive Substances Transfection Zona Fasciculata
spellingShingle	Adrenocortical cells Antioxidants Haeme oxygenase-1 High glucose Nitric oxide Oxidative stress Steroids arginine corticotropin glucose glutathione heme oxygenase 1 lipid peroxide nitrite progesterone reactive oxygen metabolite arginine glucose heme oxygenase nitrite progesterone reactive oxygen metabolite thiobarbituric acid reactive substance adrenal cortex cell animal cell article controlled study enzyme activity enzyme inhibition gene silencing hormone response hyperglycemia mouse nonhuman nucleotide sequence oxidative stress priority journal progesterone synthesis protein expression rat steroidogenesis analysis of variance animal cell clone cell culture comparative study cytology dose response genetic transfection human metabolism nonparametric test oxidative stress physiology zona fasciculata Analysis of Variance Animals Arginine Cells, Cultured Clone Cells Dose-Response Relationship, Drug Glucose Heme Oxygenase (Decyclizing) Humans Hyperglycemia Mice Nitrites Oxidative Stress Progesterone Rats Reactive Oxygen Species Statistics, Nonparametric Thiobarbituric Acid Reactive Substances Transfection Zona Fasciculata Astort, F. Repetto, E.M. Martínez Calejman, C. Cipelli, J.M. Sánchez, R. Di Gruccio, J.M. Mercau, M. Pignataro, O.P. Arias, P. Cymeryng, C.B. High glucose-induced changes in steroid production in adrenal cells
topic_facet	Adrenocortical cells Antioxidants Haeme oxygenase-1 High glucose Nitric oxide Oxidative stress Steroids arginine corticotropin glucose glutathione heme oxygenase 1 lipid peroxide nitrite progesterone reactive oxygen metabolite arginine glucose heme oxygenase nitrite progesterone reactive oxygen metabolite thiobarbituric acid reactive substance adrenal cortex cell animal cell article controlled study enzyme activity enzyme inhibition gene silencing hormone response hyperglycemia mouse nonhuman nucleotide sequence oxidative stress priority journal progesterone synthesis protein expression rat steroidogenesis analysis of variance animal cell clone cell culture comparative study cytology dose response genetic transfection human metabolism nonparametric test oxidative stress physiology zona fasciculata Analysis of Variance Animals Arginine Cells, Cultured Clone Cells Dose-Response Relationship, Drug Glucose Heme Oxygenase (Decyclizing) Humans Hyperglycemia Mice Nitrites Oxidative Stress Progesterone Rats Reactive Oxygen Species Statistics, Nonparametric Thiobarbituric Acid Reactive Substances Transfection Zona Fasciculata
description	Background: Increased activity of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in enhanced adrenocorticotropin (ACTH) and serum glucocorticoid levels, has been described in patients with diabetes mellitus and in animal models of this disease; however, altered steroid production by adrenocortical cells could result from local changes triggered by increased reactive oxygen species (ROS), induced in turn by chronic hyperglycaemia. Experiments were designed (1) to analyse the effects of incubating murine adrenocortical cells in hyperglycaemic media on the generation of oxidative stress, on steroid synthesis and on its modulation by the activity of haeme oxygenase (HO); and (2) to evaluate the effect of antioxidant treatment on these parameters. Methods: Y1 cells were incubated for 7 days with either normal or high glucose (HG, 30 mmol/L) concentrations, with or without antioxidant treatment. Parameters of oxidative stress and expression levels of haeme oxygenase-1 (HO-1), nitrite levels, L-arginine uptake and progesterone production were determined. Results: HG augmented ROS and lipoperoxide production, decreasing glutathione (GSH) levels and increasing antioxidant enzymes and HO-1 expression. Basal progesterone production was reduced, while a higher response to ACTH was observed in HG-treated cells. The increase in HO-1 expression and the effects on basal steroid production were abolished by antioxidant treatment. Inhibition of HO activity increased basal and ACTH-stimulated steroid release. Similar results were obtained by HO-1 gene silencing while the opposite effect was observed in Y1 cells overexpressing HO-1. Conclusions?: HG induces oxidative stress and affects steroid production in adrenal cells; the involvement of HO activity in the modulation of steroidogenesis in Y1 cells is postulated. Copyright © 2009 John Wiley & Sons, Ltd.
format	JOUR
author	Astort, F. Repetto, E.M. Martínez Calejman, C. Cipelli, J.M. Sánchez, R. Di Gruccio, J.M. Mercau, M. Pignataro, O.P. Arias, P. Cymeryng, C.B.
author_facet	Astort, F. Repetto, E.M. Martínez Calejman, C. Cipelli, J.M. Sánchez, R. Di Gruccio, J.M. Mercau, M. Pignataro, O.P. Arias, P. Cymeryng, C.B.
author_sort	Astort, F.
title	High glucose-induced changes in steroid production in adrenal cells
title_short	High glucose-induced changes in steroid production in adrenal cells
title_full	High glucose-induced changes in steroid production in adrenal cells
title_fullStr	High glucose-induced changes in steroid production in adrenal cells
title_full_unstemmed	High glucose-induced changes in steroid production in adrenal cells
title_sort	high glucose-induced changes in steroid production in adrenal cells
url	http://hdl.handle.net/20.500.12110/paper_15207552_v25_n5_p477_Astort
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_version_	1782027395299016704

High glucose-induced changes in steroid production in adrenal cells

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