Photochemistry of norharmane in aqueous solution
The photochemistry of norharmane (9H-pyrido[3,4-b]indole) in acidic (pH 5.0 ± 0.1) and alkaline (pH 10.0 ± 0.1) aqueous solutions was studied. The photochemical reactions were monitored by TLC, UV/VIS absorption spectroscopy, high-performance liquid chromatography (HPLC), electronic ionization-mass...
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todo:paper_1474905X_v8_n8_p1139_Gonzalez2023-10-03T16:18:35Z Photochemistry of norharmane in aqueous solution Gonzalez, M.M. Salum, M.L. Gholipour, Y. Cabrerizo, F.M. Erra-Balsells, R. beta carboline hydrogen peroxide aqueous solution article atmosphere high performance liquid chromatography mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry molecular stability photochemistry priority journal quantum yield thin layer chromatography ultraviolet spectroscopy Harmine Hydrogen Peroxide Hydrogen-Ion Concentration Mass Spectrometry Mutagens Oxygen Photochemistry Reactive Oxygen Species Singlet Oxygen Solutions Spectrometry, Fluorescence Water The photochemistry of norharmane (9H-pyrido[3,4-b]indole) in acidic (pH 5.0 ± 0.1) and alkaline (pH 10.0 ± 0.1) aqueous solutions was studied. The photochemical reactions were monitored by TLC, UV/VIS absorption spectroscopy, high-performance liquid chromatography (HPLC), electronic ionization-mass spectrometry (EI-MS), UV-laser desorption/ionization-time of flight-mass spectrometry (UV-LDI-TOF-MS) and an enzymatic method for H 2O2 determination. The neutral (nHoN) and the protonated (nHoH+) forms of norharmane irradiated under Ar atmosphere were photostable, but they suffered a photochemical transformation in the presence of O2, yielding as photoproducts norharmane dimers, trimers and tetramers. nHoN shown to be more photostable than nHoH+. The nHoH+ and nHoN consumption quantum yields were 1.82 × 10 -3 and 0.51 × 10-3, respectively, and the mechanisms involved in its photochemistry are discussed. H2O2 and singlet oxygen (1O2) were also detected and quantified in irradiated solutions of norharmane, and their role in the photochemistry of norharmane is discussed. © 2009 The Royal Society of Chemistry and Owner Societies. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1474905X_v8_n8_p1139_Gonzalez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
beta carboline hydrogen peroxide aqueous solution article atmosphere high performance liquid chromatography mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry molecular stability photochemistry priority journal quantum yield thin layer chromatography ultraviolet spectroscopy Harmine Hydrogen Peroxide Hydrogen-Ion Concentration Mass Spectrometry Mutagens Oxygen Photochemistry Reactive Oxygen Species Singlet Oxygen Solutions Spectrometry, Fluorescence Water |
spellingShingle |
beta carboline hydrogen peroxide aqueous solution article atmosphere high performance liquid chromatography mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry molecular stability photochemistry priority journal quantum yield thin layer chromatography ultraviolet spectroscopy Harmine Hydrogen Peroxide Hydrogen-Ion Concentration Mass Spectrometry Mutagens Oxygen Photochemistry Reactive Oxygen Species Singlet Oxygen Solutions Spectrometry, Fluorescence Water Gonzalez, M.M. Salum, M.L. Gholipour, Y. Cabrerizo, F.M. Erra-Balsells, R. Photochemistry of norharmane in aqueous solution |
topic_facet |
beta carboline hydrogen peroxide aqueous solution article atmosphere high performance liquid chromatography mass spectrometry matrix assisted laser desorption ionization time of flight mass spectrometry molecular stability photochemistry priority journal quantum yield thin layer chromatography ultraviolet spectroscopy Harmine Hydrogen Peroxide Hydrogen-Ion Concentration Mass Spectrometry Mutagens Oxygen Photochemistry Reactive Oxygen Species Singlet Oxygen Solutions Spectrometry, Fluorescence Water |
description |
The photochemistry of norharmane (9H-pyrido[3,4-b]indole) in acidic (pH 5.0 ± 0.1) and alkaline (pH 10.0 ± 0.1) aqueous solutions was studied. The photochemical reactions were monitored by TLC, UV/VIS absorption spectroscopy, high-performance liquid chromatography (HPLC), electronic ionization-mass spectrometry (EI-MS), UV-laser desorption/ionization-time of flight-mass spectrometry (UV-LDI-TOF-MS) and an enzymatic method for H 2O2 determination. The neutral (nHoN) and the protonated (nHoH+) forms of norharmane irradiated under Ar atmosphere were photostable, but they suffered a photochemical transformation in the presence of O2, yielding as photoproducts norharmane dimers, trimers and tetramers. nHoN shown to be more photostable than nHoH+. The nHoH+ and nHoN consumption quantum yields were 1.82 × 10 -3 and 0.51 × 10-3, respectively, and the mechanisms involved in its photochemistry are discussed. H2O2 and singlet oxygen (1O2) were also detected and quantified in irradiated solutions of norharmane, and their role in the photochemistry of norharmane is discussed. © 2009 The Royal Society of Chemistry and Owner Societies. |
format |
JOUR |
author |
Gonzalez, M.M. Salum, M.L. Gholipour, Y. Cabrerizo, F.M. Erra-Balsells, R. |
author_facet |
Gonzalez, M.M. Salum, M.L. Gholipour, Y. Cabrerizo, F.M. Erra-Balsells, R. |
author_sort |
Gonzalez, M.M. |
title |
Photochemistry of norharmane in aqueous solution |
title_short |
Photochemistry of norharmane in aqueous solution |
title_full |
Photochemistry of norharmane in aqueous solution |
title_fullStr |
Photochemistry of norharmane in aqueous solution |
title_full_unstemmed |
Photochemistry of norharmane in aqueous solution |
title_sort |
photochemistry of norharmane in aqueous solution |
url |
http://hdl.handle.net/20.500.12110/paper_1474905X_v8_n8_p1139_Gonzalez |
work_keys_str_mv |
AT gonzalezmm photochemistryofnorharmaneinaqueoussolution AT salumml photochemistryofnorharmaneinaqueoussolution AT gholipoury photochemistryofnorharmaneinaqueoussolution AT cabrerizofm photochemistryofnorharmaneinaqueoussolution AT errabalsellsr photochemistryofnorharmaneinaqueoussolution |
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1782025232340484096 |