Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients
Emission fluorescence spectra were obtained for the adaxial and abaxial faces of dicotyledonous (Ficus benjamina L., Ficus elastica, Gardenia jasminoides and Hedera helix) and monocotyledonous leaves (Gladiolus spp. and Dracaena cincta bicolor). After correction by light-re-absorption processes, usi...
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todo:paper_1474905X_v6_n8_p873_Cordon2023-10-03T16:18:31Z Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients Cordón, G.B. Lagorio, M.G. chlorophyll absorption absorption spectroscopy article chlorophyll fluorescence correlation analysis cotyledon Dracaena Ficus fluorescence spectroscopy light absorption low temperature mathematical model nonhuman optics photosystem I photosystem II plant leaf priority journal senescence Chlorophyll Fluorescence Optics Plant Leaves Dicotyledoneae Dracaena (angiosperm) Ficus benjamina Ficus elastica Gardenia angusta Gladiolus Hedera helix Liliopsida Emission fluorescence spectra were obtained for the adaxial and abaxial faces of dicotyledonous (Ficus benjamina L., Ficus elastica, Gardenia jasminoides and Hedera helix) and monocotyledonous leaves (Gladiolus spp. and Dracaena cincta bicolor). After correction by light-re-absorption processes, using a previously published physical model, the adaxial faces of dicotyledons showed a fluorescence ratio Fred/Ffar-red rather lower than the respective values for the abaxial faces. Monocotyledons and shade-adapted-plants showed similar values for the corrected fluorescence ratio for both faces. Even when differences in experimental fluorescence emission from adaxial and abaxial leaves in dicotyledons are mostly due to light re-absorption processes, the residual dissimilarity found after application of the correction model would point to the fact that fluorescence re-absorption is not the only responsible for the observed disparity. It was concluded that light re-absorption processes does not account entirely for the differences in the experimental emission spectra between adaxial and abaxial leaves. Differences that remains still present after correction might be interpreted in terms of a different photosystem ratio (PSII/PSI). Experiments at low temperature sustained this hypothesis. In dicotyledons, light reflectance for adaxial leaves was found to be lower than for the abaxial ones. It was mainly due to an increase in the scattering coefficient for the lower leaf-side. The absorption coefficient values were slightly higher for the upper leaf-side. During senescence of Ficus benjamina leaves, the scattering coefficient increased for both the upper and lower leaf-sides. With senescence time the absorption coefficient spectra broadened while the corrected fluorescence ratio (Fred/F far-red) decreased for both faces. The results pointed to a preferential destruction of photosystem II relative to photosystem I during senescence. © The Royal Society of Chemistry and Owner Societies. Fil:Cordón, G.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1474905X_v6_n8_p873_Cordon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
chlorophyll absorption absorption spectroscopy article chlorophyll fluorescence correlation analysis cotyledon Dracaena Ficus fluorescence spectroscopy light absorption low temperature mathematical model nonhuman optics photosystem I photosystem II plant leaf priority journal senescence Chlorophyll Fluorescence Optics Plant Leaves Dicotyledoneae Dracaena (angiosperm) Ficus benjamina Ficus elastica Gardenia angusta Gladiolus Hedera helix Liliopsida |
| spellingShingle |
chlorophyll absorption absorption spectroscopy article chlorophyll fluorescence correlation analysis cotyledon Dracaena Ficus fluorescence spectroscopy light absorption low temperature mathematical model nonhuman optics photosystem I photosystem II plant leaf priority journal senescence Chlorophyll Fluorescence Optics Plant Leaves Dicotyledoneae Dracaena (angiosperm) Ficus benjamina Ficus elastica Gardenia angusta Gladiolus Hedera helix Liliopsida Cordón, G.B. Lagorio, M.G. Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| topic_facet |
chlorophyll absorption absorption spectroscopy article chlorophyll fluorescence correlation analysis cotyledon Dracaena Ficus fluorescence spectroscopy light absorption low temperature mathematical model nonhuman optics photosystem I photosystem II plant leaf priority journal senescence Chlorophyll Fluorescence Optics Plant Leaves Dicotyledoneae Dracaena (angiosperm) Ficus benjamina Ficus elastica Gardenia angusta Gladiolus Hedera helix Liliopsida |
| description |
Emission fluorescence spectra were obtained for the adaxial and abaxial faces of dicotyledonous (Ficus benjamina L., Ficus elastica, Gardenia jasminoides and Hedera helix) and monocotyledonous leaves (Gladiolus spp. and Dracaena cincta bicolor). After correction by light-re-absorption processes, using a previously published physical model, the adaxial faces of dicotyledons showed a fluorescence ratio Fred/Ffar-red rather lower than the respective values for the abaxial faces. Monocotyledons and shade-adapted-plants showed similar values for the corrected fluorescence ratio for both faces. Even when differences in experimental fluorescence emission from adaxial and abaxial leaves in dicotyledons are mostly due to light re-absorption processes, the residual dissimilarity found after application of the correction model would point to the fact that fluorescence re-absorption is not the only responsible for the observed disparity. It was concluded that light re-absorption processes does not account entirely for the differences in the experimental emission spectra between adaxial and abaxial leaves. Differences that remains still present after correction might be interpreted in terms of a different photosystem ratio (PSII/PSI). Experiments at low temperature sustained this hypothesis. In dicotyledons, light reflectance for adaxial leaves was found to be lower than for the abaxial ones. It was mainly due to an increase in the scattering coefficient for the lower leaf-side. The absorption coefficient values were slightly higher for the upper leaf-side. During senescence of Ficus benjamina leaves, the scattering coefficient increased for both the upper and lower leaf-sides. With senescence time the absorption coefficient spectra broadened while the corrected fluorescence ratio (Fred/F far-red) decreased for both faces. The results pointed to a preferential destruction of photosystem II relative to photosystem I during senescence. © The Royal Society of Chemistry and Owner Societies. |
| format |
JOUR |
| author |
Cordón, G.B. Lagorio, M.G. |
| author_facet |
Cordón, G.B. Lagorio, M.G. |
| author_sort |
Cordón, G.B. |
| title |
Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| title_short |
Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| title_full |
Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| title_fullStr |
Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| title_full_unstemmed |
Optical properties of the adaxial and abaxial faces of leaves. Chlorophyll fluorescence, absorption and scattering coefficients |
| title_sort |
optical properties of the adaxial and abaxial faces of leaves. chlorophyll fluorescence, absorption and scattering coefficients |
| url |
http://hdl.handle.net/20.500.12110/paper_1474905X_v6_n8_p873_Cordon |
| work_keys_str_mv |
AT cordongb opticalpropertiesoftheadaxialandabaxialfacesofleaveschlorophyllfluorescenceabsorptionandscatteringcoefficients AT lagoriomg opticalpropertiesoftheadaxialandabaxialfacesofleaveschlorophyllfluorescenceabsorptionandscatteringcoefficients |
| _version_ |
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