Virus infection elevates transcriptional activity of miR164a promoter in plants
Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-tran...
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todo:paper_14712229_v9_n_p_Bazzini2023-10-03T16:17:52Z Virus infection elevates transcriptional activity of miR164a promoter in plants Bazzini, A.A. Almasia, N.I. Manacorda, C.A. Mongelli, V.C. Conti, G. Maroniche, G.A. Rodriguez, M.C. Distéfano, A.J. Hopp, H.E. Del Vas, M. Asurmendi, S. Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results. Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion. This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. © 2009 Bazzini et al; licensee BioMed Central Ltd. Fil:Bazzini, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Almasia, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mongelli, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroniche, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Asurmendi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14712229_v9_n_p_Bazzini |
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Universidad de Buenos Aires |
institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant |
spellingShingle |
Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Bazzini, A.A. Almasia, N.I. Manacorda, C.A. Mongelli, V.C. Conti, G. Maroniche, G.A. Rodriguez, M.C. Distéfano, A.J. Hopp, H.E. Del Vas, M. Asurmendi, S. Virus infection elevates transcriptional activity of miR164a promoter in plants |
topic_facet |
Arabidopsis Brassica napus Chinese Rape Mosaic Virus Tobacco mosaic virus microRNA microRNA 164, Arabidopsis microRNA-164, Arabidopsis phytohormone plant RNA Arabidopsis article biology gene expression regulation genetics metabolism molecular cloning Mosaic virus physiology plant disease promoter region reporter gene transgenic plant virology Arabidopsis Cloning, Molecular Computational Biology Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Reporter MicroRNAs Mosaic Viruses Plant Diseases Plant Growth Regulators Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant |
description |
Background. Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results. Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion. This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. © 2009 Bazzini et al; licensee BioMed Central Ltd. |
format |
JOUR |
author |
Bazzini, A.A. Almasia, N.I. Manacorda, C.A. Mongelli, V.C. Conti, G. Maroniche, G.A. Rodriguez, M.C. Distéfano, A.J. Hopp, H.E. Del Vas, M. Asurmendi, S. |
author_facet |
Bazzini, A.A. Almasia, N.I. Manacorda, C.A. Mongelli, V.C. Conti, G. Maroniche, G.A. Rodriguez, M.C. Distéfano, A.J. Hopp, H.E. Del Vas, M. Asurmendi, S. |
author_sort |
Bazzini, A.A. |
title |
Virus infection elevates transcriptional activity of miR164a promoter in plants |
title_short |
Virus infection elevates transcriptional activity of miR164a promoter in plants |
title_full |
Virus infection elevates transcriptional activity of miR164a promoter in plants |
title_fullStr |
Virus infection elevates transcriptional activity of miR164a promoter in plants |
title_full_unstemmed |
Virus infection elevates transcriptional activity of miR164a promoter in plants |
title_sort |
virus infection elevates transcriptional activity of mir164a promoter in plants |
url |
http://hdl.handle.net/20.500.12110/paper_14712229_v9_n_p_Bazzini |
work_keys_str_mv |
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