Glucocorticoid-induced apoptosis: A simple set of laboratory experiments

Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced a...

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Detalles Bibliográficos
Autores principales: Vicent, G.P., Lanuza, G.M., Romero, D.G., Di Croce, L., Pecci, A.
Formato: JOUR
Materias:
corticosterone
dexamethasone
DNA fragment
glucocorticoid
glucocorticoid receptor
mifepristone
progesterone
testosterone
animal cell
apoptosis
concentration response
conference paper
drug effect
male
nonhuman
rat
thymocyte
thymus
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14708175_v28_n6_p307_Vicent
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14708175_v28_n6_p307_Vicent
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spelling todo:paper_14708175_v28_n6_p307_Vicent2023-10-03T16:17:41Z Glucocorticoid-induced apoptosis: A simple set of laboratory experiments Vicent, G.P. Lanuza, G.M. Romero, D.G. Di Croce, L. Pecci, A. corticosterone dexamethasone DNA fragment glucocorticoid glucocorticoid receptor mifepristone progesterone testosterone animal cell apoptosis concentration response conference paper drug effect male nonhuman rat thymocyte thymus Animalia Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced apoptosis in the thymus. The set of experiments that we propose to perform and discuss are relatively simple and can be rapidly carried out in a course for undergraduates. This practice also allows the students to familiarize themselves with basic pharmacology, compare in vivo vs. in vitro assays and acquire skills in animal handling. The organisation of the practical work requires 4-5h of experimental training in each of two days. Copyright © 2001 IUBMB. Fil:Vicent, G.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14708175_v28_n6_p307_Vicent
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic corticosterone
dexamethasone
DNA fragment
glucocorticoid
glucocorticoid receptor
mifepristone
progesterone
testosterone
animal cell
apoptosis
concentration response
conference paper
drug effect
male
nonhuman
rat
thymocyte
thymus
Animalia
spellingShingle corticosterone
dexamethasone
DNA fragment
glucocorticoid
glucocorticoid receptor
mifepristone
progesterone
testosterone
animal cell
apoptosis
concentration response
conference paper
drug effect
male
nonhuman
rat
thymocyte
thymus
Animalia
Vicent, G.P.
Lanuza, G.M.
Romero, D.G.
Di Croce, L.
Pecci, A.
Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
topic_facet corticosterone
dexamethasone
DNA fragment
glucocorticoid
glucocorticoid receptor
mifepristone
progesterone
testosterone
animal cell
apoptosis
concentration response
conference paper
drug effect
male
nonhuman
rat
thymocyte
thymus
Animalia
description Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced apoptosis in the thymus. The set of experiments that we propose to perform and discuss are relatively simple and can be rapidly carried out in a course for undergraduates. This practice also allows the students to familiarize themselves with basic pharmacology, compare in vivo vs. in vitro assays and acquire skills in animal handling. The organisation of the practical work requires 4-5h of experimental training in each of two days. Copyright © 2001 IUBMB.
format JOUR
author Vicent, G.P.
Lanuza, G.M.
Romero, D.G.
Di Croce, L.
Pecci, A.
author_facet Vicent, G.P.
Lanuza, G.M.
Romero, D.G.
Di Croce, L.
Pecci, A.
author_sort Vicent, G.P.
title Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
title_short Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
title_full Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
title_fullStr Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
title_full_unstemmed Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
title_sort glucocorticoid-induced apoptosis: a simple set of laboratory experiments
url http://hdl.handle.net/20.500.12110/paper_14708175_v28_n6_p307_Vicent
work_keys_str_mv AT vicentgp glucocorticoidinducedapoptosisasimplesetoflaboratoryexperiments
AT lanuzagm glucocorticoidinducedapoptosisasimplesetoflaboratoryexperiments
AT romerodg glucocorticoidinducedapoptosisasimplesetoflaboratoryexperiments
AT dicrocel glucocorticoidinducedapoptosisasimplesetoflaboratoryexperiments
AT peccia glucocorticoidinducedapoptosisasimplesetoflaboratoryexperiments
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