Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat

Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost underst...

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Autores principales:	Ferder, I., Parborell, F., Sundblad, V., Chiauzzi, V., Gómez, K., Charreau, E.H., Tesone, M., Dain, L.
Formato:	JOUR
Materias:	fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_14701626_v145_n4_p335_Ferder
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spelling	todo:paper_14701626_v145_n4_p335_Ferder2023-10-03T16:17:36Z Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat Ferder, I. Parborell, F. Sundblad, V. Chiauzzi, V. Gómez, K. Charreau, E.H. Tesone, M. Dain, L. fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development. © 2013 Society for Reproduction and Fertility. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sundblad, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger
spellingShingle	fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger Ferder, I. Parborell, F. Sundblad, V. Chiauzzi, V. Gómez, K. Charreau, E.H. Tesone, M. Dain, L. Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
topic_facet	fragile X mental retardation protein messenger RNA animal experiment animal tissue article brain cytoplasm female germ cell granulosa cell immunohistochemistry nonhuman ovary follicle ovary follicle development priority journal protein expression rat testis theca cell Animals Female Fragile X Mental Retardation Protein Ovarian Follicle Protein Isoforms Rats Rats, Sprague-Dawley RNA, Messenger
description	Fragile X mental retardation protein (FMRP) belongs to a small family of RNA-binding proteins. Its absence or inactivity is responsible for fragile X syndrome, the most common cause of inherited mental retardation. Despite its ubiquitous expression, FMRP function and expression remain almost understudied in non-neuronal tissues, though previous studies on germline development during oogenesis may suggest a special function of this protein also in ovarian tissue. In addition, the well-documented association of FMR1 premutation state with fragile X-related premature ovarian insufficiency adds interest to the role of FMRP in ovarian physiology. The aim of the present work was to investigate the expression of Fmr1 mRNA and its protein, FMRP, at different stages of rat follicular development. By immunohistochemical studies we demonstrated FMRP expression in granulosa, theca and germ cells in all stages of follicular development. In addition, changes in Fmr1 expression, both at the protein and mRNA levels, were observed. FMRP levels increased upon follicular development while preantral and early antral follicles presented similar levels of Fmr1 transcripts with decreased expression in preovulatory follicles. These observations suggest that Fmr1 expression in the ovary is regulated at different and perhaps independent levels. In addition, our results show expression of at least four different isoforms of FMRP during all stages of follicular growth with expression patterns that differ from those observed in brain and testis. Our study shows a regulated expression of Fmr1, both at mRNA and protein levels, during rat follicular development. © 2013 Society for Reproduction and Fertility.
format	JOUR
author	Ferder, I. Parborell, F. Sundblad, V. Chiauzzi, V. Gómez, K. Charreau, E.H. Tesone, M. Dain, L.
author_facet	Ferder, I. Parborell, F. Sundblad, V. Chiauzzi, V. Gómez, K. Charreau, E.H. Tesone, M. Dain, L.
author_sort	Ferder, I.
title	Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
title_short	Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
title_full	Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
title_fullStr	Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
title_full_unstemmed	Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat
title_sort	expression of fragile x mental retardation protein and fmr1 mrna during folliculogenesis in the rat
url	http://hdl.handle.net/20.500.12110/paper_14701626_v145_n4_p335_Ferder
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Expression of fragile X mental retardation protein and Fmr1 mRNA during folliculogenesis in the rat

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