Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction

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Bovine sperm protease, 66 kDa (BSp66) is a serine protease previously characterized in bovine spermatozoa. Like other proteases, it may be present in sperm from other mammalian species different from bovine, playing a role in the fertilization process. In this study, we looked for BSp66 in hamster s...

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Autores principales: Cesari, A., Katunar, M.R., Monclus, M.A., Vincenti, A., de Rosas, J.C., Fornés, M.W.
Formato: JOUR
Lenguaje:English
Materias:
antigen
isoprotein
polyclonal antibody
serine proteinase
acrosome reaction
animal cell
animal experiment
animal tissue
antigen antibody reaction
article
cell function
controlled study
cow
electron microscopy
enzyme activity
female
fertilization
fertilization in vitro
hamster
immunofluorescence
immunoreactivity
male
molecular weight
nonhuman
nucleotide sequence
priority journal
protein localization
sequence homology
signal transduction
spermatozoon
spermatozoon count
zona pellucida
Animals
Cattle
Cell Culture Techniques
Cell Survival
Cricetinae
Fertilization in Vitro
Immunohistochemistry
Male
Microscopy, Immunoelectron
Serine Endopeptidases
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa
Bovinae
Mammalia
Mesocricetus auratus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14701626_v129_n3_p291_Cesari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_14701626_v129_n3_p291_Cesari2023-10-03T16:17:33Z Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction Cesari, A. Katunar, M.R. Monclus, M.A. Vincenti, A. de Rosas, J.C. Fornés, M.W. antigen isoprotein polyclonal antibody serine proteinase acrosome reaction animal cell animal experiment animal tissue antigen antibody reaction article cell function controlled study cow electron microscopy enzyme activity female fertilization fertilization in vitro hamster immunofluorescence immunoreactivity male molecular weight nonhuman nucleotide sequence priority journal protein localization sequence homology signal transduction spermatozoon spermatozoon count zona pellucida Animals Cattle Cell Culture Techniques Cell Survival Cricetinae Fertilization in Vitro Immunohistochemistry Male Microscopy, Immunoelectron Serine Endopeptidases Sperm Motility Sperm-Ovum Interactions Spermatozoa Bovinae Cricetinae Mammalia Mesocricetus auratus Bovine sperm protease, 66 kDa (BSp66) is a serine protease previously characterized in bovine spermatozoa. Like other proteases, it may be present in sperm from other mammalian species different from bovine, playing a role in the fertilization process. In this study, we looked for BSp66 in hamster spermatozoa using heterologous antibodies against bovine BSp66. An immunoreactive protein was detected by Western blotting in mature and immature sperm. The detected protein had two isoforms similar to the ones reported in bovine sperm. Furthermore, indirect immune detection by fluorescence and electron microscopy assays, showed BSp66 signal at the acrosomal region similar to bovine sperm. As it was determined in bovine sperm, the acrosomal reaction displays the antigen within the acrosomal content. When live hamster sperm was incubated with polyclonal antibody against bovine BSp66 a decrease in the number of sperm bound to zona pellucida in homologous IVF and an impairment of head-head agglutination, were observed. These results suggest that a protease homologous to bovine BSp66 is present in golden hamster spermatozoa, with a conserved molecular mass and cellular location. Moreover, hamster BSp66 is probably involved in zona pellucida recognition. © 2005 Society for Reproduction and Fertility. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v129_n3_p291_Cesari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language English
orig_language_str_mv English
topic antigen
isoprotein
polyclonal antibody
serine proteinase
acrosome reaction
animal cell
animal experiment
animal tissue
antigen antibody reaction
article
cell function
controlled study
cow
electron microscopy
enzyme activity
female
fertilization
fertilization in vitro
hamster
immunofluorescence
immunoreactivity
male
molecular weight
nonhuman
nucleotide sequence
priority journal
protein localization
sequence homology
signal transduction
spermatozoon
spermatozoon count
zona pellucida
Animals
Cattle
Cell Culture Techniques
Cell Survival
Cricetinae
Fertilization in Vitro
Immunohistochemistry
Male
Microscopy, Immunoelectron
Serine Endopeptidases
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa
Bovinae
Cricetinae
Mammalia
Mesocricetus auratus
spellingShingle antigen
isoprotein
polyclonal antibody
serine proteinase
acrosome reaction
animal cell
animal experiment
animal tissue
antigen antibody reaction
article
cell function
controlled study
cow
electron microscopy
enzyme activity
female
fertilization
fertilization in vitro
hamster
immunofluorescence
immunoreactivity
male
molecular weight
nonhuman
nucleotide sequence
priority journal
protein localization
sequence homology
signal transduction
spermatozoon
spermatozoon count
zona pellucida
Animals
Cattle
Cell Culture Techniques
Cell Survival
Cricetinae
Fertilization in Vitro
Immunohistochemistry
Male
Microscopy, Immunoelectron
Serine Endopeptidases
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa
Bovinae
Cricetinae
Mammalia
Mesocricetus auratus
Cesari, A.
Katunar, M.R.
Monclus, M.A.
Vincenti, A.
de Rosas, J.C.
Fornés, M.W.
Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
topic_facet antigen
isoprotein
polyclonal antibody
serine proteinase
acrosome reaction
animal cell
animal experiment
animal tissue
antigen antibody reaction
article
cell function
controlled study
cow
electron microscopy
enzyme activity
female
fertilization
fertilization in vitro
hamster
immunofluorescence
immunoreactivity
male
molecular weight
nonhuman
nucleotide sequence
priority journal
protein localization
sequence homology
signal transduction
spermatozoon
spermatozoon count
zona pellucida
Animals
Cattle
Cell Culture Techniques
Cell Survival
Cricetinae
Fertilization in Vitro
Immunohistochemistry
Male
Microscopy, Immunoelectron
Serine Endopeptidases
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa
Bovinae
Cricetinae
Mammalia
Mesocricetus auratus
description Bovine sperm protease, 66 kDa (BSp66) is a serine protease previously characterized in bovine spermatozoa. Like other proteases, it may be present in sperm from other mammalian species different from bovine, playing a role in the fertilization process. In this study, we looked for BSp66 in hamster spermatozoa using heterologous antibodies against bovine BSp66. An immunoreactive protein was detected by Western blotting in mature and immature sperm. The detected protein had two isoforms similar to the ones reported in bovine sperm. Furthermore, indirect immune detection by fluorescence and electron microscopy assays, showed BSp66 signal at the acrosomal region similar to bovine sperm. As it was determined in bovine sperm, the acrosomal reaction displays the antigen within the acrosomal content. When live hamster sperm was incubated with polyclonal antibody against bovine BSp66 a decrease in the number of sperm bound to zona pellucida in homologous IVF and an impairment of head-head agglutination, were observed. These results suggest that a protease homologous to bovine BSp66 is present in golden hamster spermatozoa, with a conserved molecular mass and cellular location. Moreover, hamster BSp66 is probably involved in zona pellucida recognition. © 2005 Society for Reproduction and Fertility.
format JOUR
author Cesari, A.
Katunar, M.R.
Monclus, M.A.
Vincenti, A.
de Rosas, J.C.
Fornés, M.W.
author_facet Cesari, A.
Katunar, M.R.
Monclus, M.A.
Vincenti, A.
de Rosas, J.C.
Fornés, M.W.
author_sort Cesari, A.
title Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
title_short Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
title_full Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
title_fullStr Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
title_full_unstemmed Serine protease activity, bovine sperm protease, 66 kDa (BSP66), is present in hamster sperm and is involved in sperm-zona interaction
title_sort serine protease activity, bovine sperm protease, 66 kda (bsp66), is present in hamster sperm and is involved in sperm-zona interaction
url http://hdl.handle.net/20.500.12110/paper_14701626_v129_n3_p291_Cesari
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AT katunarmr serineproteaseactivitybovinespermprotease66kdabsp66ispresentinhamsterspermandisinvolvedinspermzonainteraction
AT monclusma serineproteaseactivitybovinespermprotease66kdabsp66ispresentinhamsterspermandisinvolvedinspermzonainteraction
AT vincentia serineproteaseactivitybovinespermprotease66kdabsp66ispresentinhamsterspermandisinvolvedinspermzonainteraction
AT derosasjc serineproteaseactivitybovinespermprotease66kdabsp66ispresentinhamsterspermandisinvolvedinspermzonainteraction
AT fornesmw serineproteaseactivitybovinespermprotease66kdabsp66ispresentinhamsterspermandisinvolvedinspermzonainteraction
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