Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor

Phytochromes constitute a major photoreceptor family found in plants, algae, fungi, and prokaryotes, including pathogens. Here, we report that Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease which affects cruciferous crops worldwide, codes for a functional bacterio...

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Autores principales:	Bonomi, H.R., Toum, L., Sycz, G., Sieira, R., Toscani, A.M., Gudesblat, G.E., Leskow, F.C., Goldbaum, F.A., Vojnov, A.A., Malamud, F.
Formato:	JOUR
Materias:	bathy-type phytochrome infection plant defenses transcriptional regulation virulence factors bacteriophytochrome exopolysaccharide phytochrome unclassified drug virulence factor xanthan bacterial polysaccharide bacterial protein amino terminal sequence Article bacterial virulence biofilm carbohydrate synthesis carboxy terminal sequence controlled study far red light illumination light nonhuman photoreceptor plant defense priority journal sequence analysis Xanthomonas campestris biosynthesis crop gene expression regulation genetics growth, development and aging high throughput sequencing metabolism microbiology mutation pathogenicity plant disease Bacterial Proteins Biofilms Crops, Agricultural Gene Expression Regulation, Bacterial High-Throughput Nucleotide Sequencing Light Mutation Phytochrome Plant Diseases Polysaccharides, Bacterial Virulence Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1469221X_v17_n11_p1565_Bonomi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1469221X_v17_n11_p1565_Bonomi
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spelling	todo:paper_1469221X_v17_n11_p1565_Bonomi2023-10-03T16:17:29Z Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor Bonomi, H.R. Toum, L. Sycz, G. Sieira, R. Toscani, A.M. Gudesblat, G.E. Leskow, F.C. Goldbaum, F.A. Vojnov, A.A. Malamud, F. bathy-type phytochrome infection plant defenses transcriptional regulation virulence factors bacteriophytochrome exopolysaccharide phytochrome unclassified drug virulence factor xanthan bacterial polysaccharide bacterial protein phytochrome amino terminal sequence Article bacterial virulence biofilm carbohydrate synthesis carboxy terminal sequence controlled study far red light illumination light nonhuman photoreceptor plant defense priority journal sequence analysis Xanthomonas campestris biosynthesis crop gene expression regulation genetics growth, development and aging high throughput sequencing light metabolism microbiology mutation pathogenicity plant disease Xanthomonas campestris Bacterial Proteins Biofilms Crops, Agricultural Gene Expression Regulation, Bacterial High-Throughput Nucleotide Sequencing Light Mutation Phytochrome Plant Diseases Polysaccharides, Bacterial Virulence Factors Xanthomonas campestris Phytochromes constitute a major photoreceptor family found in plants, algae, fungi, and prokaryotes, including pathogens. Here, we report that Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease which affects cruciferous crops worldwide, codes for a functional bacteriophytochrome (XccBphP). XccBphP possesses an N-terminal PAS2-GAF-PHY photosensory domain triad and a C-terminal PAS9 domain as its output module. Our results show that illumination of Xcc, prior to plant infection, attenuates its virulence in an XccBphP-dependent manner. Moreover, in response to light, XccBphP downregulates xanthan exopolysaccharide production and biofilm formation, two known Xcc virulence factors. Furthermore, the XccbphP null mutant shows enhanced virulence, similar to that of dark-adapted Xcc cultures. Stomatal aperture regulation and callose deposition, both well-established plant defense mechanisms against bacterial pathogens, are overridden by the XccbphP strain. Additionally, an RNA-Seq analysis reveals that far-red light or XccBphP overexpression produces genomewide transcriptional changes, including the inhibition of several Xcc virulence systems. Our findings indicate that Xcc senses light through XccBphP, eliciting bacterial virulence attenuation via downregulation of bacterial virulence factors. The capacity of XccBphP to respond to light both in vitro and in vivo was abolished by a mutation on the conserved Cys13 residue. These results provide evidence for a novel bacteriophytochrome function affecting an infectious process. © 2016 The Authors Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vojnov, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Malamud, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1469221X_v17_n11_p1565_Bonomi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bathy-type phytochrome infection plant defenses transcriptional regulation virulence factors bacteriophytochrome exopolysaccharide phytochrome unclassified drug virulence factor xanthan bacterial polysaccharide bacterial protein phytochrome amino terminal sequence Article bacterial virulence biofilm carbohydrate synthesis carboxy terminal sequence controlled study far red light illumination light nonhuman photoreceptor plant defense priority journal sequence analysis Xanthomonas campestris biosynthesis crop gene expression regulation genetics growth, development and aging high throughput sequencing light metabolism microbiology mutation pathogenicity plant disease Xanthomonas campestris Bacterial Proteins Biofilms Crops, Agricultural Gene Expression Regulation, Bacterial High-Throughput Nucleotide Sequencing Light Mutation Phytochrome Plant Diseases Polysaccharides, Bacterial Virulence Factors Xanthomonas campestris
spellingShingle	bathy-type phytochrome infection plant defenses transcriptional regulation virulence factors bacteriophytochrome exopolysaccharide phytochrome unclassified drug virulence factor xanthan bacterial polysaccharide bacterial protein phytochrome amino terminal sequence Article bacterial virulence biofilm carbohydrate synthesis carboxy terminal sequence controlled study far red light illumination light nonhuman photoreceptor plant defense priority journal sequence analysis Xanthomonas campestris biosynthesis crop gene expression regulation genetics growth, development and aging high throughput sequencing light metabolism microbiology mutation pathogenicity plant disease Xanthomonas campestris Bacterial Proteins Biofilms Crops, Agricultural Gene Expression Regulation, Bacterial High-Throughput Nucleotide Sequencing Light Mutation Phytochrome Plant Diseases Polysaccharides, Bacterial Virulence Factors Xanthomonas campestris Bonomi, H.R. Toum, L. Sycz, G. Sieira, R. Toscani, A.M. Gudesblat, G.E. Leskow, F.C. Goldbaum, F.A. Vojnov, A.A. Malamud, F. Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
topic_facet	bathy-type phytochrome infection plant defenses transcriptional regulation virulence factors bacteriophytochrome exopolysaccharide phytochrome unclassified drug virulence factor xanthan bacterial polysaccharide bacterial protein phytochrome amino terminal sequence Article bacterial virulence biofilm carbohydrate synthesis carboxy terminal sequence controlled study far red light illumination light nonhuman photoreceptor plant defense priority journal sequence analysis Xanthomonas campestris biosynthesis crop gene expression regulation genetics growth, development and aging high throughput sequencing light metabolism microbiology mutation pathogenicity plant disease Xanthomonas campestris Bacterial Proteins Biofilms Crops, Agricultural Gene Expression Regulation, Bacterial High-Throughput Nucleotide Sequencing Light Mutation Phytochrome Plant Diseases Polysaccharides, Bacterial Virulence Factors Xanthomonas campestris
description	Phytochromes constitute a major photoreceptor family found in plants, algae, fungi, and prokaryotes, including pathogens. Here, we report that Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease which affects cruciferous crops worldwide, codes for a functional bacteriophytochrome (XccBphP). XccBphP possesses an N-terminal PAS2-GAF-PHY photosensory domain triad and a C-terminal PAS9 domain as its output module. Our results show that illumination of Xcc, prior to plant infection, attenuates its virulence in an XccBphP-dependent manner. Moreover, in response to light, XccBphP downregulates xanthan exopolysaccharide production and biofilm formation, two known Xcc virulence factors. Furthermore, the XccbphP null mutant shows enhanced virulence, similar to that of dark-adapted Xcc cultures. Stomatal aperture regulation and callose deposition, both well-established plant defense mechanisms against bacterial pathogens, are overridden by the XccbphP strain. Additionally, an RNA-Seq analysis reveals that far-red light or XccBphP overexpression produces genomewide transcriptional changes, including the inhibition of several Xcc virulence systems. Our findings indicate that Xcc senses light through XccBphP, eliciting bacterial virulence attenuation via downregulation of bacterial virulence factors. The capacity of XccBphP to respond to light both in vitro and in vivo was abolished by a mutation on the conserved Cys13 residue. These results provide evidence for a novel bacteriophytochrome function affecting an infectious process. © 2016 The Authors
format	JOUR
author	Bonomi, H.R. Toum, L. Sycz, G. Sieira, R. Toscani, A.M. Gudesblat, G.E. Leskow, F.C. Goldbaum, F.A. Vojnov, A.A. Malamud, F.
author_facet	Bonomi, H.R. Toum, L. Sycz, G. Sieira, R. Toscani, A.M. Gudesblat, G.E. Leskow, F.C. Goldbaum, F.A. Vojnov, A.A. Malamud, F.
author_sort	Bonomi, H.R.
title	Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
title_short	Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
title_full	Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
title_fullStr	Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
title_full_unstemmed	Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
title_sort	xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor
url	http://hdl.handle.net/20.500.12110/paper_1469221X_v17_n11_p1565_Bonomi
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_version_	1807315756344410112

Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor

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