Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops
Summary: It is expected that the next generation of biotech crops displaying enhanced quality traits with benefits to both farmers and consumers will have a better acceptance than first generation biotech crops and will improve public perception of genetic engineering. This will only be true if they...
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todo:paper_14677644_v9_n2_p136_Llorente2023-10-03T16:17:25Z Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops Llorente, B. Alonso, G.D. Bravo-Almonacid, F. Rodríguez, V. López, M.G. Carrari, F. Torres, H.N. Flawiá, M.M. Biotech crop GMO safety assessment Polyphenol oxidase Potato quality improvement Solanum tuberosum Tuber browning allergen catechol oxidase animal article Bagg albino mouse biology female food safety gene silencing genetic engineering genetics mouse photosynthesis potato Allergens Animals Catechol Oxidase Computational Biology Female Food Safety Gene Silencing Genetic Engineering Mice Mice, Inbred BALB C Photosynthesis Solanum tuberosum Solanum tuberosum Tuber (truffle) Summary: It is expected that the next generation of biotech crops displaying enhanced quality traits with benefits to both farmers and consumers will have a better acceptance than first generation biotech crops and will improve public perception of genetic engineering. This will only be true if they are proven to be as safe as traditionally bred crops. In contrast with the first generation of biotech crops where only a single trait is modified, the next generation of biotech crops will add a new level of complexity inherent to the mechanisms underlying their output traits. In this study, a comprehensive evaluation of the comparative safety approach on a quality-improved biotech crop with metabolic modifications is presented. Three genetically engineered potato lines with silenced polyphenol oxidase (Ppo) transcripts and reduced tuber browning were characterized at both physiological and molecular levels and showed to be equivalent to wild-type (WT) plants when yield-associated traits and photosynthesis were evaluated. Analysis of the primary metabolism revealed several unintended metabolic modifications in the engineered tubers, providing evidence for potential compositional inequivalence between transgenic lines and WT controls. The silencing construct sequence was in silico analysed for potential allergenic cross-reactivity, and no similarities to known allergenic proteins were identified. Moreover, in vivo intake safety evaluation showed no adverse effects in physiological parameters. Taken together, these results provide the first evidence supporting that the safety of next generation biotech crops can be properly assessed following the current evaluation criterion, even if the transgenic and WT crops are not substantially equivalent. © 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd. Fil:Llorente, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:López, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carrari, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14677644_v9_n2_p136_Llorente |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biotech crop GMO safety assessment Polyphenol oxidase Potato quality improvement Solanum tuberosum Tuber browning allergen catechol oxidase animal article Bagg albino mouse biology female food safety gene silencing genetic engineering genetics mouse photosynthesis potato Allergens Animals Catechol Oxidase Computational Biology Female Food Safety Gene Silencing Genetic Engineering Mice Mice, Inbred BALB C Photosynthesis Solanum tuberosum Solanum tuberosum Tuber (truffle) |
| spellingShingle |
Biotech crop GMO safety assessment Polyphenol oxidase Potato quality improvement Solanum tuberosum Tuber browning allergen catechol oxidase animal article Bagg albino mouse biology female food safety gene silencing genetic engineering genetics mouse photosynthesis potato Allergens Animals Catechol Oxidase Computational Biology Female Food Safety Gene Silencing Genetic Engineering Mice Mice, Inbred BALB C Photosynthesis Solanum tuberosum Solanum tuberosum Tuber (truffle) Llorente, B. Alonso, G.D. Bravo-Almonacid, F. Rodríguez, V. López, M.G. Carrari, F. Torres, H.N. Flawiá, M.M. Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| topic_facet |
Biotech crop GMO safety assessment Polyphenol oxidase Potato quality improvement Solanum tuberosum Tuber browning allergen catechol oxidase animal article Bagg albino mouse biology female food safety gene silencing genetic engineering genetics mouse photosynthesis potato Allergens Animals Catechol Oxidase Computational Biology Female Food Safety Gene Silencing Genetic Engineering Mice Mice, Inbred BALB C Photosynthesis Solanum tuberosum Solanum tuberosum Tuber (truffle) |
| description |
Summary: It is expected that the next generation of biotech crops displaying enhanced quality traits with benefits to both farmers and consumers will have a better acceptance than first generation biotech crops and will improve public perception of genetic engineering. This will only be true if they are proven to be as safe as traditionally bred crops. In contrast with the first generation of biotech crops where only a single trait is modified, the next generation of biotech crops will add a new level of complexity inherent to the mechanisms underlying their output traits. In this study, a comprehensive evaluation of the comparative safety approach on a quality-improved biotech crop with metabolic modifications is presented. Three genetically engineered potato lines with silenced polyphenol oxidase (Ppo) transcripts and reduced tuber browning were characterized at both physiological and molecular levels and showed to be equivalent to wild-type (WT) plants when yield-associated traits and photosynthesis were evaluated. Analysis of the primary metabolism revealed several unintended metabolic modifications in the engineered tubers, providing evidence for potential compositional inequivalence between transgenic lines and WT controls. The silencing construct sequence was in silico analysed for potential allergenic cross-reactivity, and no similarities to known allergenic proteins were identified. Moreover, in vivo intake safety evaluation showed no adverse effects in physiological parameters. Taken together, these results provide the first evidence supporting that the safety of next generation biotech crops can be properly assessed following the current evaluation criterion, even if the transgenic and WT crops are not substantially equivalent. © 2010 The Authors. Plant Biotechnology Journal © 2010 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd. |
| format |
JOUR |
| author |
Llorente, B. Alonso, G.D. Bravo-Almonacid, F. Rodríguez, V. López, M.G. Carrari, F. Torres, H.N. Flawiá, M.M. |
| author_facet |
Llorente, B. Alonso, G.D. Bravo-Almonacid, F. Rodríguez, V. López, M.G. Carrari, F. Torres, H.N. Flawiá, M.M. |
| author_sort |
Llorente, B. |
| title |
Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| title_short |
Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| title_full |
Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| title_fullStr |
Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| title_full_unstemmed |
Safety assessment of nonbrowning potatoes: Opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| title_sort |
safety assessment of nonbrowning potatoes: opening the discussion about the relevance of substantial equivalence on next generation biotech crops |
| url |
http://hdl.handle.net/20.500.12110/paper_14677644_v9_n2_p136_Llorente |
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1807316090198425600 |