Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants

Snakin-1 (SN1), a cysteine-rich peptide with broad-spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highl...

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Detalles Bibliográficos
Autores principales: Almasia, N.I., Bazzini, A.A., Hopp, H.E., Vazquez-Rovere, C.
Formato: JOUR
Materias:
Agrobacterium tumefaciens
Hyphomycetes
Pectobacterium carotovorum
Solanum
Solanum chacoense
Solanum tuberosum
Thanatephorus cucumeris
Tuberosum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14646722_v9_n3_p329_Almasia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14646722_v9_n3_p329_Almasia
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spelling todo:paper_14646722_v9_n3_p329_Almasia2023-10-03T16:17:17Z Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants Almasia, N.I. Bazzini, A.A. Hopp, H.E. Vazquez-Rovere, C. Agrobacterium tumefaciens Hyphomycetes Pectobacterium carotovorum Solanum Solanum chacoense Solanum tuberosum Thanatephorus cucumeris Tuberosum Snakin-1 (SN1), a cysteine-rich peptide with broad-spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91-98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping-off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild-type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application. © 2008 Blackwell publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14646722_v9_n3_p329_Almasia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Agrobacterium tumefaciens
Hyphomycetes
Pectobacterium carotovorum
Solanum
Solanum chacoense
Solanum tuberosum
Thanatephorus cucumeris
Tuberosum
spellingShingle Agrobacterium tumefaciens
Hyphomycetes
Pectobacterium carotovorum
Solanum
Solanum chacoense
Solanum tuberosum
Thanatephorus cucumeris
Tuberosum
Almasia, N.I.
Bazzini, A.A.
Hopp, H.E.
Vazquez-Rovere, C.
Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
topic_facet Agrobacterium tumefaciens
Hyphomycetes
Pectobacterium carotovorum
Solanum
Solanum chacoense
Solanum tuberosum
Thanatephorus cucumeris
Tuberosum
description Snakin-1 (SN1), a cysteine-rich peptide with broad-spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91-98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping-off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild-type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application. © 2008 Blackwell publishing Ltd.
format JOUR
author Almasia, N.I.
Bazzini, A.A.
Hopp, H.E.
Vazquez-Rovere, C.
author_facet Almasia, N.I.
Bazzini, A.A.
Hopp, H.E.
Vazquez-Rovere, C.
author_sort Almasia, N.I.
title Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
title_short Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
title_full Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
title_fullStr Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
title_full_unstemmed Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
title_sort overexpression of snakin-1 gene enhances resistance to rhizoctonia solani and erwinia carotovora in transgenic potato plants
url http://hdl.handle.net/20.500.12110/paper_14646722_v9_n3_p329_Almasia
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