ArcA redox mutants as a source of reduced bioproducts
Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generatio...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari |
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todo:paper_14641801_v15_n1_p41_Pettinari2023-10-03T16:17:06Z ArcA redox mutants as a source of reduced bioproducts Pettinari, M.J. Nikel, P.I. Ruiz, J.A. Méndez, B.S. ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generation pathway according to the intracellular redox state. High throughput studies on different redox regulator mutants, involving transcriptome analysis, RT-PCR and phenotypic arrays enabled the elucidation of a repertoire of operons coordinated by ArcA which extended beyond respiration control including, among others, those which code for survival, chromosome replication and degradation of fatty acids. Flux analysis by 13C labeling provided new clues to the understanding of the distribution of metabolites mediated by ArcAB. The genetic manipulation of this regulator proved to be useful for the generation of reduced products of commercial value. Copyright © 2008 S. Karger AG. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli |
| spellingShingle |
ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli Pettinari, M.J. Nikel, P.I. Ruiz, J.A. Méndez, B.S. ArcA redox mutants as a source of reduced bioproducts |
| topic_facet |
ArcA mutants ArcAB ArcAB modulon Metabolic flux analysis Polyhydroxybutyrate fatty acid transcriptome chromosome replication Escherichia coli gene isolation gene mutation genetic trait phenotype real time polymerase chain reaction respiration control review Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Escherichia coli Proteins Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Regulator Oxidation-Reduction Oxygen Repressor Proteins Signal Transduction Arca Escherichia coli |
| description |
Escherichia coli and other facultative anaerobes can adapt their metabolism according to oxygen availability by means of aerobic and anaerobic respiration and fermentation. ArcAB is a two-component signal transduction system that controls, at the transcriptional level, the choice of energy generation pathway according to the intracellular redox state. High throughput studies on different redox regulator mutants, involving transcriptome analysis, RT-PCR and phenotypic arrays enabled the elucidation of a repertoire of operons coordinated by ArcA which extended beyond respiration control including, among others, those which code for survival, chromosome replication and degradation of fatty acids. Flux analysis by 13C labeling provided new clues to the understanding of the distribution of metabolites mediated by ArcAB. The genetic manipulation of this regulator proved to be useful for the generation of reduced products of commercial value. Copyright © 2008 S. Karger AG. |
| format |
JOUR |
| author |
Pettinari, M.J. Nikel, P.I. Ruiz, J.A. Méndez, B.S. |
| author_facet |
Pettinari, M.J. Nikel, P.I. Ruiz, J.A. Méndez, B.S. |
| author_sort |
Pettinari, M.J. |
| title |
ArcA redox mutants as a source of reduced bioproducts |
| title_short |
ArcA redox mutants as a source of reduced bioproducts |
| title_full |
ArcA redox mutants as a source of reduced bioproducts |
| title_fullStr |
ArcA redox mutants as a source of reduced bioproducts |
| title_full_unstemmed |
ArcA redox mutants as a source of reduced bioproducts |
| title_sort |
arca redox mutants as a source of reduced bioproducts |
| url |
http://hdl.handle.net/20.500.12110/paper_14641801_v15_n1_p41_Pettinari |
| work_keys_str_mv |
AT pettinarimj arcaredoxmutantsasasourceofreducedbioproducts AT nikelpi arcaredoxmutantsasasourceofreducedbioproducts AT ruizja arcaredoxmutantsasasourceofreducedbioproducts AT mendezbs arcaredoxmutantsasasourceofreducedbioproducts |
| _version_ |
1807323604721860608 |