Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis
Primate genomes show a great karyological variability while the DNA content variation is scarce. The biggest genome size occurs in Cercophitecus cephus (Catarrhini, Cercophitecidae) with 5.26 pg whereas the smallest one is described for Callicebus torquatus (Platyrrhini, Callithricidae) with 2.26 pg...
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| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14248581_v128_n4_p214_Nieves |
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todo:paper_14248581_v128_n4_p214_Nieves2023-10-03T16:13:48Z Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis Nieves, M. Mühlmann, M. Mudry, M.D. Cebus nigritus Cebus paraguayanus Comparative genomic hybridization Genome size Heterochromatin Platyrrhini Primate cytogenetics repetitive DNA animal cell animal genetics article Cebidae Cebus nigritus Cebus paraguayanus chromosome pairing comparative genomic hybridization controlled study euchromatin female genome analysis heterochromatin male nonhuman priority journal species comparison species difference telomere Animals Argentina Cebus Cercopithecus Comparative Genomic Hybridization DNA Female Genome Humans Karyotyping Male Primates Species Specificity Callicebus torquatus Callitrichidae Catarrhini Cebidae Cebus Cephus Platyrrhini Primates Primate genomes show a great karyological variability while the DNA content variation is scarce. The biggest genome size occurs in Cercophitecus cephus (Catarrhini, Cercophitecidae) with 5.26 pg whereas the smallest one is described for Callicebus torquatus (Platyrrhini, Callithricidae) with 2.26 pg. Over the last 20 years different authors have been studying the Platyrrhini genomes on a chromosomal level. Among them, Cebus (Cebidae) being considered the most ancestral and conserved karyotype in relation to human karyotype has been extensively studied. Cebus genome sizes range from 3.40 to 3.98 pg. The species that inhabit Argentina, where they reach the most southern natural distribution, Cebus paraguayanus (CPA) and Cebus nigritus (CNI), have been extensively studied with classical cytogenetic comparisons focusing on banding pattern behavior. In the present study we performed comparative genomic hybridization (CGH) between these two closely related species with the aim of going a step further in the dissection of Cebus genomes. CGH evidenced that the DNA imbalances between them involved different genome regions, i.e. preferentially repetitive DNA in CPA and coding or very disperse DNA in CNI. Particularly, CNI showed species-specific DNA in more than 9 chromosomal pairs with a red/green (r/g) ratio ranging from 1.7 to 4, meaning that CNI presents at least twice as much DNA than CPA in those chromosomal segments. CPA showed species-specific DNA in the telomeric region of at least 3 chromosomal pairs with an r/g ratio of 0.5. They also showed a DNA gain in the chromosomal pairs with extracentromeric heterochromatin. Our findings modify the widespread idea of considering the heterochromatin proportion as the only difference between CPA and CNI. In Cebus then, the diversification process could be mediated by little changes in DNA content accompanied by a euchromatin-heterochromatin interaction although maintaining a minimum proportion like the one observed in CNI. Copyright © 2010 S. Karger AG, Basel. Fil:Nieves, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14248581_v128_n4_p214_Nieves |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Cebus nigritus Cebus paraguayanus Comparative genomic hybridization Genome size Heterochromatin Platyrrhini Primate cytogenetics repetitive DNA animal cell animal genetics article Cebidae Cebus nigritus Cebus paraguayanus chromosome pairing comparative genomic hybridization controlled study euchromatin female genome analysis heterochromatin male nonhuman priority journal species comparison species difference telomere Animals Argentina Cebus Cercopithecus Comparative Genomic Hybridization DNA Female Genome Humans Karyotyping Male Primates Species Specificity Callicebus torquatus Callitrichidae Catarrhini Cebidae Cebus Cephus Platyrrhini Primates |
| spellingShingle |
Cebus nigritus Cebus paraguayanus Comparative genomic hybridization Genome size Heterochromatin Platyrrhini Primate cytogenetics repetitive DNA animal cell animal genetics article Cebidae Cebus nigritus Cebus paraguayanus chromosome pairing comparative genomic hybridization controlled study euchromatin female genome analysis heterochromatin male nonhuman priority journal species comparison species difference telomere Animals Argentina Cebus Cercopithecus Comparative Genomic Hybridization DNA Female Genome Humans Karyotyping Male Primates Species Specificity Callicebus torquatus Callitrichidae Catarrhini Cebidae Cebus Cephus Platyrrhini Primates Nieves, M. Mühlmann, M. Mudry, M.D. Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| topic_facet |
Cebus nigritus Cebus paraguayanus Comparative genomic hybridization Genome size Heterochromatin Platyrrhini Primate cytogenetics repetitive DNA animal cell animal genetics article Cebidae Cebus nigritus Cebus paraguayanus chromosome pairing comparative genomic hybridization controlled study euchromatin female genome analysis heterochromatin male nonhuman priority journal species comparison species difference telomere Animals Argentina Cebus Cercopithecus Comparative Genomic Hybridization DNA Female Genome Humans Karyotyping Male Primates Species Specificity Callicebus torquatus Callitrichidae Catarrhini Cebidae Cebus Cephus Platyrrhini Primates |
| description |
Primate genomes show a great karyological variability while the DNA content variation is scarce. The biggest genome size occurs in Cercophitecus cephus (Catarrhini, Cercophitecidae) with 5.26 pg whereas the smallest one is described for Callicebus torquatus (Platyrrhini, Callithricidae) with 2.26 pg. Over the last 20 years different authors have been studying the Platyrrhini genomes on a chromosomal level. Among them, Cebus (Cebidae) being considered the most ancestral and conserved karyotype in relation to human karyotype has been extensively studied. Cebus genome sizes range from 3.40 to 3.98 pg. The species that inhabit Argentina, where they reach the most southern natural distribution, Cebus paraguayanus (CPA) and Cebus nigritus (CNI), have been extensively studied with classical cytogenetic comparisons focusing on banding pattern behavior. In the present study we performed comparative genomic hybridization (CGH) between these two closely related species with the aim of going a step further in the dissection of Cebus genomes. CGH evidenced that the DNA imbalances between them involved different genome regions, i.e. preferentially repetitive DNA in CPA and coding or very disperse DNA in CNI. Particularly, CNI showed species-specific DNA in more than 9 chromosomal pairs with a red/green (r/g) ratio ranging from 1.7 to 4, meaning that CNI presents at least twice as much DNA than CPA in those chromosomal segments. CPA showed species-specific DNA in the telomeric region of at least 3 chromosomal pairs with an r/g ratio of 0.5. They also showed a DNA gain in the chromosomal pairs with extracentromeric heterochromatin. Our findings modify the widespread idea of considering the heterochromatin proportion as the only difference between CPA and CNI. In Cebus then, the diversification process could be mediated by little changes in DNA content accompanied by a euchromatin-heterochromatin interaction although maintaining a minimum proportion like the one observed in CNI. Copyright © 2010 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Nieves, M. Mühlmann, M. Mudry, M.D. |
| author_facet |
Nieves, M. Mühlmann, M. Mudry, M.D. |
| author_sort |
Nieves, M. |
| title |
Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| title_short |
Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| title_full |
Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| title_fullStr |
Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| title_full_unstemmed |
Cebus paraguayanus and cebus nigritus (primates, platyrrhini): A comparative genomic hybridization analysis |
| title_sort |
cebus paraguayanus and cebus nigritus (primates, platyrrhini): a comparative genomic hybridization analysis |
| url |
http://hdl.handle.net/20.500.12110/paper_14248581_v128_n4_p214_Nieves |
| work_keys_str_mv |
AT nievesm cebusparaguayanusandcebusnigritusprimatesplatyrrhiniacomparativegenomichybridizationanalysis AT muhlmannm cebusparaguayanusandcebusnigritusprimatesplatyrrhiniacomparativegenomichybridizationanalysis AT mudrymd cebusparaguayanusandcebusnigritusprimatesplatyrrhiniacomparativegenomichybridizationanalysis |
| _version_ |
1807316699202977792 |